Mucilaginibacter oryzae sp. nov., isolated from soil of a rice paddy

Abstract

A Gram-negative-staining, non-spore-forming bacterium devoid of flagella, designated strain B9 T , was isolated from rice paddy soil associated with the roots of Oryza sativa collected from Jinju, South Korea. Cells were straight rods, were catalase-and oxidase-positive and were able to hydrolyse pectin, xylan and laminarin. Growth of strain B9 T was observed between 15 and 35 6C (optimum 25-30 6C) and between pH 5.0 and 8.0 (optimum pH 6.5-7.5). Strain B9 T contained menaquinone-7 (MK-7) as a major isoprenoid quinone and summed feature 3 (C 16 : 1 v7c and/or iso-C 15 : 0 2-OH), iso-C 15 : 0 and C 16 : 0 as major fatty acids. The G+C content of the genomic DNA was 44.4 mol%. Comparative 16S rRNA gene sequence analysis showed that strain B9 T belonged to the genus Mucilaginibacter, a member of the family Sphingobacteriaceae, and was most closely related to Mucilaginibacter kameinonensis SCK T (95.9 % sequence similarity). O