Abstract Milk fat contains 400 vital fatty acids beneficial for human health. Special attention is given to fatty acids (FAs) that could play a positive role for human health; such are butyric, oleic acid, caproic, caprylic and capric acids. Keeping the medicinal properties of milk fatty acids in consideration, goat milk samples were analyzed for estimation of fatty acid contents in Indian goat milk by using gas chromatography. Analysis of goat milk samples revealed the highest concentration saturated fatty acids (SFA) out of total milk fatty acids (FA) with an average of 69.55 g/100g of fatty acid methyl ester (FAME) ranging from 43.26 to 88.05g/100g of FAME. Within saturated fatty acid the major contribution was given by palmitic (C16:0) 26.99% followed by myristic (C14:0) 11.77%, stearic (C18:0) 7.66% and capric (C10:0) 6.75% respectively. The concentration of short chain fatty acids (SCFAs, C4 to C10) was found to be 13.51 g/100g varying from 2.23 to 33.63 g/100g of FAME. Whereas the concentration of medium chain fatty acids (MCFAs , C12to C15) was 20.05% varying from 7.470 to 45.27 g/100 g of FAME and Long chain FA (LFA, C16 to C24) was 35.08% varying from 4.77 to 51.22 g/100g of FAME. The average concentration of unsaturated fatty acids (UFAs) was 28.50 g/100grm of FAME varying from 10.44 to 45.74 g/100g of FAME which includes monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) with an average of 24.57 g/100g of FAME ranges varying from 4.79 to 39.40 g/100g of FAME and 3.96 g/100g of FAME ranges varying from 0.5928 to 18.30g/100 g of FAME, respectively
