Development and Validation of an RP-HPLC Method for Methionine, Cystine and Lysine Separation and Determination in Corn Samples

Abstract

In this paper a RP-HPLC method is developed and validated. Using a Hypersil BDS C18 column, a mobile phase: solvent A (phosphate buffer) and solvent B (water : acetonitrile : methanol, 20:20:60 v/v/v), a 45°C column temperature and a flow rate of 1.7 mL/min, the proposed method proved a good separation of the investigated amino acids, in 35 minutes