Abstract: Polymorphism in the banding profiles of the electrophoretic separation of seed proteins are used to reassess the taxonomic relationships between 21 samples representing eight species of the genus Orobanche L. (Orobanchaceae) that were collected from different cultivated and wild host in Egypt and Saudi Arabia. A high degree of similarity in the banding profile of samples of the same species is revealed in the studied taxa confirming the validity of seed proteins as a source of taxonomic criteria. Moreover, nine bands are common to all samples of the studied 21 species which may be indicative of their common origin and support the view that the genus Orobanche is a monophyletic group. The analysis of results using the NTSYS-pc program and the UPGMA and NJ clustering methods support the delimitation of species of Orobanche in two sections Orobanche W allr. and Trionychon wallr. Also, supports delimitation of the studied species in section Orobanche in three groups. Moreover the delimitation of the studied species belonging to section Trionychon in two groups supports the previous delimitation of this section