Classification of metamorphic virus using n-grams signatures

Metamorphic virus has a capability to change, translate, and rewrite its own code once infected the system to bypass detection. The computer system then can be seriously damage by this undetected metamorphic virus. Due to this, it is very vital to design a metamorphic virus classification model that can detect this virus. This paper focused on detection of metamorphic virus using Term Frequency Inverse Document Frequency (TF-IDF) technique. This research was conducted using Second Generation virus dataset. The first step is the classification model to cluster the metamorphic virus using TF-IDF technique. Then, the virus cluster is evaluated using Naïve Bayes algorithm in terms of accuracy using performance metric. The types of virus classes and features are extracted from bi-gram assembly language. The result shows that the proposed model was able to classify metamorphic virus using TF-IDF with optimal number of virus class with average accuracy of 94.2%

The Malaise of the Administrative Process

Computer viruses uses a few different techniques, with various intentions, toinfect files. However, what most of them have in common is that they wantto avoid detection by anti-malware software. To not get detected and stay unnoticed,virus creators have developed several methods for this. Anti-malwaresoftware is constantly trying to counter these methods of virus infections withtheir own detection-techniques. In this paper we have analyzed the differenttypes of viruses and their infection techniques, and tried to determined whichworks the best to avoid detection. In the experiments we have done we havesimulated executing the viruses at the same time as an anti-malware softwarewas running. Our conclusion is that metamorphic viruses uses the best methodsto stay unnoticed by anti-malware software’s detection techniques

Water system virus detection

A monitoring system developed to test the capability of a water recovery system to reject the passage of viruses into the recovered water is described. A nonpathogenic marker virus, bacteriophage F2, is fed into the process stream before the recovery unit and the reclaimed water is assayed for its presence. Detection of the marker virus consists of two major components, concentration and isolation of the marker virus, and detection of the marker virus. The concentration system involves adsorption of virus to cellulose acetate filters in the presence of trivalent cations and low pH with subsequent desorption of the virus using volumes of high pH buffer. The detection of the virus is performed by a passive immune agglutination test utilizing specially prepared polystyrene particles. An engineering preliminary design was performed as a parallel effort to the laboratory development of the marker virus test system. Engineering schematics and drawings of a fully functional laboratory prototype capable of zero-G operation are presented. The instrument consists of reagent pump/metering system, reagent storage containers, a filter concentrator, an incubation/detector system, and an electronic readout and control system

Modern tools for safe detection of diseases in sugarcane quarantine

Aims: Use of modern molecular tools in disease detection, different detection methods, visual observation, serological tests, PCR or RT-PCR tests have been described for elimination of pests and diseases from infected sugarcane germplasm. Application of strict quarantine measures to control movement of sugarcane germplasm is advocated. Methods and Results: Improvement of sugar crops relies, to a large extent, upon the cultivation of new sugarcane varieties that are either bred locally or imported from other geographical locations. In either case, there is a need for importing sugarcane germplasm from abroad. Because sugarcane is vegetatively propagated (stem cuttings, tissue-cultured plantlets), there is a high risk of introducing infectious diseases or pests from countries of origin. Therefore, strict quarantine measures must be applied to control movement of sugarcane germplasm. In the past, quarantine procedures relied almost exclusively upon the cultivation of imported varieties in isolated and closed premises (greenhouses), and the access to these areas was limited to a few duly authorized persons. Additionally, visual search for disease symptoms was performed during the entire growth cycle of the plants. These procedures are still in use at present time, but a wide range of tools for detecting and controlling diseases have been developed during the last decades, resulting in quarantine practices that are much safer and reliable. Nowadays, most of the efforts must be focused on the detection and elimination of symptomless or latent diseases such as leaf scald (Xanthomonas albilineans), ratoon stunting (Leifsonia xyli subsp. xyli) or yellow leaf (Sugarcane yellow leaf virus or SCYLV). Additionally, special attention must be paid to emerging diseases, the symptoms of which are sometimes rather unusual or difficult to detect, and for which efficient detection tools are not always available. Finally, detection tools must be very efficient in detecting low populations of the pathogen as well as all variants of this pathogen. Within the last decade, CIRAD's sugarcane quarantine had to face two emerging diseases, streak mosaic (Sugarcane streak mosaic virus or SCSMV) and yellow leaf. SCSMV appeared to be a heterogeneous virus, and none of the antisera used allowed us to detect all the isolates of our collection. Fortunately, an RT-PCR test developed in our laboratory has been quite sensitive and efficient. Since the end of the 1990s, sugarcane yellow leaf has been routinely detected in our quarantine using a tissue blot immuno-assay (TBIA) and RT-PCR tests. However, recent studies of the genetic diversity of SCYLV showed that some isolates of this virus were not systematically detected, and new and more universal primers were designed. A similar situation was experienced for the detection of sugarcane mosaic, a disease caused by two variable viruses: Sugarcane mosaic virus (SCMV) and Sorghum mosaic virus (SrMV). Following genetic diversity studies of SCMV, including virus isolates from various geographical origins, new primer pairs were designed and used in RT-PCR. Significance of study: Even though modem molecular tools have greatly improved disease detection in sugarcane quarantine, their exclusive use is not advisable. Very often, a combination of different detection methods must be performed: visual observation of the plants at several growth stages, isolation of bacterial pathogens on selective media, serological tests, PCR or RT-PCR tests. Additionally, the use of physical (hot water or hot air), chemical (fungicide and pesticide) treatments and apical meristem culture allow the elimination of many pests and diseases from infected sugarcane germplasm. (Texte intégral

Metagenomic screening of the sugarcane virome in Florida. [P.36]

Viral metagenomics has revolutionized the way pathologists decipher viral diseases. While the impact of this new approach is still debatable in plant virus diagnostics, viral metagenomics has already produced key advances in viral ecology and has the potential to become a central approach for viral surveillance at the ecosystem scale. A viral metagenomics study of the sugarcane virome in Florida was carried out in 2013/2014. One hundred and eighty sugarcane leaf samples were collected from different commercial sugarcane (Saccharum interspecific hybrids) fields in Florida and from other Saccharum and related species taken from two local germplasm collections. Sequence-independent next generation sequencing (NGS) of virion-associated nucleic acids (VANA) was used for detection and identification of viruses present within the collected leaf samples. All four previously reported sugarcane viruses occuring in Florida were detected: Sugarcane yellow leaf virus (149 infected samples out of 180), Sugarcane mosaic virus (2/180), Sugarcane mild mosaic virus (10/180) and Sugarcane bacilliform virus (51/180). Interestingly, this viral metagenomics approach also resulted in the detection of potential new viruses of sugarcane, including Chrysovirus, Mastrevirus, and Umbravirus. This study provided a snapshot vision of the SCYLV genetic diversity in 2013/2014 in Florida where several genotypes of this virus are present. It also allowed us to assemble the whole genome of at least one new mastrevirus species. (Résumé d'auteur

Differentiation of Foot-and-Mouth Disease-Infected pigs from Vaccinated Pigs Using Antibody-Detecting Sandwich ELISA

The presence of serum antibodies for nonstructural proteins of the foot-and-mouth disease virus (FMDV) can differentiate FMDV-infected animals from vaccinated animals. In this study, a sandwich ELISA was developed for rapid detection of the foot-and-mouth disease (FMD) antibodies; it was based on an Escherichia coli-expressed, highly conserved region of the 3ABC nonstructural protein of the FMDV O/TW/99 strain and a monoclonal antibody derived from the expressed protein. The diagnostic sensitivity of the assay was 98.4%, and the diagnostic specificity was 100% for naïve and vaccinated pigs; the detection ability of the assay was comparable those of the PrioCHECK and UBI kits. There was 97.5, 93.4 and 66.6% agreement between the results obtained from our ELISA and those obtained from the PrioCHECK, UBI and CHEKIT kits, respectively. The kappa statistics were 0.95, 0.87 and 0.37, respectively. Moreover, antibodies for nonstructural proteins of the serotypes A, C, Asia 1, SAT 1, SAT 2 and SAT 3 were also detected in bovine sera. Furthermore, the absence of cross-reactions generated by different antibody titers against the swine vesicular disease virus and vesicular stomatitis virus (VSV) was also highlighted in this assay's specificit

A two-step lyssavirus real-time polymerase chain reaction using degenerate primers with superior sensitivity to the fluorescent antigen test

A generic two-step lyssavirus real-time reverse transcriptase polymerase chain reaction (qRT-PCR), based on a nested PCR strategy, was validated for the detection of different lyssavirus species. Primers with 17 to 30% of degenerate bases were used in both consecutive steps. The assay could accurately detect RABV, LBV, MOKV, DUVV, EBLV-1, EBLV-2, and ABLV. In silico sequence alignment showed a functional match with the remaining lyssavirus species. The diagnostic specificity was 100% and the sensitivity proved to be superior to that of the fluorescent antigen test. The limit of detection was <= 1 50% tissue culture infectious dose. The related vesicular stomatitis virus was not recognized, confirming the selectivity for lyssaviruses. The assay was applied to follow the evolution of rabies virus infection in the brain of mice from 0 to 10 days after intranasal inoculation. The obtained RNA curve corresponded well with the curves obtained by a one-step monospecific RABV-qRT-PCR, the fluorescent antigen test, and virus titration. Despite the presence of degenerate bases, the assay proved to be highly sensitive, specific, and reproducible

Water system virus detection

The performance of a waste water reclamation system is monitored by introducing a non-pathogenic marker virus, bacteriophage F2, into the waste-water prior to treatment and, thereafter, testing the reclaimed water for the presence of the marker virus. A test sample is first concentrated by absorbing any marker virus onto a cellulose acetate filter in the presence of a trivalent cation at low pH and then flushing the filter with a limited quantity of a glycine buffer solution to desorb any marker virus present on the filter. Photo-optical detection of indirect passive immune agglutination by polystyrene beads indicates the performance of the water reclamation system in removing the marker virus. A closed system provides for concentrating any marker virus, initiating and monitoring the passive immune agglutination reaction, and then flushing the system to prepare for another sample

CMV in the gut: a critical review of CMV detection in the immunocompetent host with colitis.

As scientific techniques for the detection of cytomegalovirus (CMV) improve, we are able to detect small amounts of CMV in the mucosal wall. As clinicians, we are unsure how to interpret the results of this novel test. There is controversy in the literature as to the significance of the detection of CMV in the gut. Whilst the importance of CMV and reactivation of the virus is clear in those patients such as allograft recipients with established immune compromise, the role is less clear in patients with less damaged immune systems. We explore whether the detection of CMV in such cases influences outcome and how it should be optimally managed. We discuss the optimal management of such cases, according to current guidelines, with a review of the literature