Growth of MRSA and Pseudomonas aeruginosa in a fine-celled foam model containing sessile commensal skin bacteria

Sessile cultures of the skin bacteria Staphylococcus saprophyticus and Corynebacterium xerosis were grown using novel fine-celled foam substrata to test the outcome of challenge by methicillin-resistant Staphylococcus aureus or Pseudomonas aeruginosa under three growth medium regimens (simulated sweat, simulated serum or simulated sweat substituted with simulated serum during the microbial challenge). S. saprophyticus and C. xerosis significantly limited MRSA and P. aeruginosa immigration respectively, under the simulated sweat and serum medium regimes. Under the substitution medium regime however, MRSA and P. aeruginosa integrated into pre-established biofilms to a significantly greater extent, attaining cell densities similar to the axenic controls. The outcome of challenge was influenced by the medium composition and test organism but could not be predicted based on planktonic competition assays or growth dynamics. Interactions between skin and wound isolates could be modelled using the fine-celled foam-based system. This model could be used to further investigate interactions and also in preclinical studies of antimicrobial wound care regimens

Technology study of passive control of humidity in space suits

Water vapor condensation and adsorption techniques for passive humidity control in space suit

The Effects of Men\u27s Lacrosse Protective Equipment on Thermoregulation and Perceptions During Exercise Heat Stress

The effects of men\u27s lacrosse protective equipment on thermoregulation and perceptions during exercise heat stress Amanda P. Glasgow: Exercise Science Research Center, University of Arkansas, Fayetteville, AR. Background: Exertional heat stroke and other heat related illnesses are a common concern for athletic coaches and players. Looking to prevent further injuries, it is questioned whether players perceive if their bodies are heating to a dangerous level. Many factors play into the body’s response and the players perception including thirst, thermoregulation, and the kind of protective equipment the athletes are wearing. Purpose: Examine if perceptual responses are different when participants wear men’s lacrosse protective equipment compared to when they do not. Our hypothesis was that reported perceptual responses would be different between equipment and non-equipment trials. Methodology: We recruited 12 healthy males ages 21.8 ± 3.35 with heights 181.29 cm ± 6.07 cm, with body fat of 14.7% ± 4.4% to complete our randomized crossover study. Participants were required to have previous equipment intensive sport experience. After a health history clearance and a familiarization session, participants completed two trials (once with and once without standard men’s lacrosse protective equipment) a simulated lacrosse exercise protocol consisting of four 12-minute sessions with rests totaling 60 minutes. Measured values included first morning urine osmolality and rating of perceived exertion (RPE), thermal sensation, thirst perception, and muscle pain perception every 15minutes throughout and following exercise. Results: There was no significant difference between trials with first morning urine osmolality (p=.128). In terms of equipment, RPE was significantly greater with equipment throughout trials (p\u3c.001) compared to the no equipment trial. Thermal sensation was significantly greater during the equipment trial versus the no equipment trial (p\u3c.001). Regardless of time point, thirst sensation was greater in the equipment trial compared to the no equipment trial (p=.001). Muscle pain was significantly greater throughout trials, regardless of time point (P=.006) in the equipment trial as well. Discussion: Overall, our study supported our hypothesis that men’s lacrosse equipment accentuates perceptual stress during exercise heat stress. Regardless of time point, RPE, thermal sensation, thirst sensation, and muscle pain were reported as greater during equipment trials than non-equipment trials. Funding Source: University of Arkansas Honors College Research Gran

Oral application of L-menthol in the heat: From pleasure to performance

When menthol is applied to the oral cavity it presents with a familiar refreshing sensation and cooling mint flavour. This may be deemed hedonic in some individuals, but may cause irritation in others. This variation in response is likely dependent upon trigeminal sensitivity toward cold stimuli, suggesting a need for a menthol solution that can be easily personalised. Menthol’s characteristics can also be enhanced by matching colour to qualitative outcomes; a factor which can easily be manipulated by practitioners working in athletic or occupational settings to potentially enhance intervention efficacy. This presentation will outline the efficacy of oral menthol application for improving time trial performance to date, either via swilling or via co-ingestion with other cooling strategies, with an emphasis upon how menthol can be applied in ecologically valid scenarios. Situations in which performance is not expected to be enhanced will also be discussed. An updated model by which menthol may prove hedonic, satiate thirst and affect ventilation will also be presented, with the potential performance implications of these findings discussed and modelled. Qualitative reflections from athletes that have implemented menthol mouth swilling in competition, training and maximal exercise will also be included

Physiological Studies of Heat Stress Acclimation During a Specific Exercise Regimen

Eleven subjects were used to determine if the exercise regimen of racquetball could be used as a heat stress acclimator. Core temperature, skin temperature, sweat production, and weight loss were recorded during a racquetball match. Skin and core temperatures were determined by using thermistors. Sweat was collected with modified stress electrodes. Weight loss was recorded by comparing nude weights at the beginning and end of a match. The results indicated that an hour of strenuous racquetball play caused a significant increase in core temperature with subsequent sweating which resulted in a significant decrease in skin temperature and weight loss. The exercise regimen of racquetball can act as a good heat stress acclimator because it produces sufficiently high levels of hyperthermia

Carbohydrate gel ingestion significantly improves the intermittent endurance capacity, but not sprint performance, of adolescent team games players during a simulated team games protocol

The aim of this study was to investigate the influence of ingesting a carbohydrate (CHO) gel on the intermittent endurance capacity and sprint performance of adolescent team games players. Eleven participants [mean age 13.5 ± 0.7 years, height 1.72 ± 0.08 m, body mass (BM) 62.1 ± 9.4 kg] performed two trials separated by 3–7 days. In each trial, they completed four 15 min periods of part A of the Loughborough Intermittent Shuttle Test (LIST), followed by an intermittent run to exhaustion (part B). In the 5 min pre-exercise, participants consumed 0.818 mL kg−1 BM of a CHO or a non-CHO placebo gel, and a further 0.327 mL kg−1 BM every 15 min during part A of the LIST (38.0 ± 5.5 g CHO h−1 in the CHO trial). Intermittent endurance capacity was increased by 21.1% during part B when the CHO gel was ingested (4.6 ± 2.0 vs. 3.8 ± 2.4 min, P < 0.05, r = 0.67), with distance covered in part B significantly greater in the CHO trial (787 ± 319 vs. 669 ± 424 m, P < 0.05, r = 0.57). Gel ingestion did not significantly influence mean 15 m sprint time (P = 0.34), peak sprint time (P = 0.81), or heart rate (P = 0.66). Ingestion of a CHO gel significantly increases the intermittent endurance capacity of adolescent team games players during a simulated team games protocol

Effect of a Carbohydrate-Electrolyte Solution on Fluid Balance and Performance at a Thermoneutral Environment in International-Level Fencers

© 2019 by the National Strength & Conditioning Association.Chryssanthopoulos, C, Tsolakis, C, Bottoms, L, Toubekis, A, Zacharogiannis, E, Pafili, Z, and Maridaki, M. Effect of a carbohydrate-electrolyte solution on fluid balance and performance at a thermoneutral environment in international-level fencers. J Strength Cond Res 34(1): 152-161, 2020-The purpose of the study was to examine a possible effect of a carbohydrate-electrolyte (CHO-E) solution on fluid balance and performance in fencing at a thermoneutral environment. Sixteen fencers performed two 120-minute training sessions separated by 7-14 days under similar environmental conditions (temperature: 20.3° C and humidity: 45-47%). Each session consisted of 60-minute conditioning exercises followed by 10 bouts of 3 minutes against the same opponent with 3-minute interval between each bout. Participants ingested at regular intervals either a 6% CHO-E solution or an artificially sweetened water (PL) in a counterbalanced order. No difference was observed between conditions in the heart rate responses, perceived exertion, changes in plasma volume, urine specific gravity, number of bouts won or lost, or points for and against. Considerable variability was observed in body mass changes that revealed significant differences at the time level (i.e., pre- vs. post-exercise) (F1,15 = 9.31, p = 0.008, η = 0.38), whereas no difference was found between conditions (i.e., CHO-E vs. PL) (F1,15 = 0.43, p = 0.52, η = 0.03) and conditions × time interaction (F1,15 = 3.57, p = 0.078, η = 0.19). Fluid loss was not significantly different between conditions (p = 0.08, d = 0.47). The blood glucose level was higher (p < 0.01) after exercise in CHO-E, whereas the blood lactate level was similar between conditions. In conclusion, the CHO-E solution was as effective as the artificially sweetened water in terms of fluid balance and fencing performance at a thermoneutral environment. Because of large individual variability, fencers should monitor their fluid intake and body fluid loss in training and competition.Peer reviewedFinal Accepted Versio

Development and fabrication of an instrument to measure integrated skin temperature Final report

Instrument to measure integrated skin temperature of human