Characterization of Rice (Oryza sativa L.) Roots Versus Root Pulling Resistance as Selection Indices for Draught Tolerance

A technique described as Root Pulling Resistance (RPR) was used to evaluate genotypic differences in root growth and development of 50 rice germplasm accessions and cultivars. Several root characteristics in rice are associated with drought tolerance and avoidance capability of plants. The RPR measurements showed a significant positive correlation with maximum root length (r=0.69), root thickness (r=0.75), branching number (r=0.75), and root dry weight (r= 0.82). Rice genotypes that had a high RPR value were identified as having longer, thicker, and denser root systems. The data indicated that high RPR measurements are strongly correlated with greater root penetration. Munji Sufaid Pak, IR52 (IR5853-1 18-5) and Saunfia or Mabla Pak 329 had a significantly greater root length, root thickness, root number, root branching and dry weight as compared to IR 36. Also, there was no correlation between plant height and RPR. Furthermore, the data demonstrated that the RPR technique is ideal for selecting superior root systems and potential drought tolerant rice germplasm and cultivars

High efficiency protoplast isolation from in vitro cultures and hairy roots of Maesa lanceolata

In vitro cultures of the medicinal plant Maesa lanceolata were established to enable the cultivation of plant material for the production of protoplasts. Callus cultures were initiated using leaves collected from shoot cultures and the root tips from hairy root cultures obtained upon Agrobacterium rhizogenes transformation. For the isolation of protoplast, the different explant material of M. lanceolata was exposed to an enzyme mixture consisting of 1.5% cellulase, 0.5% macerozyme R-10 and 0.5 M mannitol. About 6 x 106 protoplasts g-1 fresh weight were obtained from leaf material and 5 x 105 protoplasts g-1 fresh weight from callus. To obtain high amounts of hairy root protoplasts, the cultures were pretreated with the auxin indole-3-butyric acid (IBA) that stimulated the formation of novel root tips. Using the dissected root tips as starting material, 8 x 105 protoplasts g-1 fresh weight were obtained per preparation. The protoplast isolation method will enable further studies on the transformation and fusion of protoplasts from M. lanceolata

Locality with staggered fermions

We address the locality problem arising in simulations, which take the square root of the staggered fermion determinant as a Boltzmann weight to reduce the number of dynamical quark tastes. A definition of such a theory necessitates an underlying local fermion operator with the same determinant and the corresponding Green's functions to establish causality and unitarity. We illustrate this point by studying analytically and numerically the square root of the staggered fermion operator. Although it has the correct weight, this operator is non-local in the continuum limit. Our work serves as a warning that fundamental properties of field theories might be violated when employing blindly the square root trick. The question, whether a local operator reproducing the square root of the staggered fermion determinant exists, is left open.Comment: 24 pages, 7 figures, few remarks added for clarity, accepted for publication in Nucl. Phys.

Affine Wa(A4), Quaternions, and Decagonal Quasicrystals

We introduce a technique of projection onto the Coxeter plane of an arbitrary higher dimensional lattice described by the affine Coxeter group. The Coxeter plane is determined by the simple roots of the Coxeter graph I2 (h) where h is the Coxeter number of the Coxeter group W(G) which embeds the dihedral group Dh of order 2h as a maximal subgroup. As a simple application we demonstrate projections of the root and weight lattices of A4 onto the Coxeter plane using the strip (canonical) projection method. We show that the crystal spaces of the affine Wa(A4) can be decomposed into two orthogonal spaces whose point groups is the dihedral group D5 which acts in both spaces faithfully. The strip projections of the root and weight lattices can be taken as models for the decagonal quasicrystals. The paper also revises the quaternionic descriptions of the root and weight lattices, described by the affine Coxeter group Wa(A3), which correspond to the face centered cubic (fcc) lattice and body centered cubic (bcc) lattice respectively. Extensions of these lattices to higher dimensions lead to the root and weight lattices of the group Wa(An), n>=4 . We also note that the projection of the Voronoi cell of the root lattice of Wa(A4) describes a framework of nested decagram growing with the power of the golden ratio recently discovered in the Islamic arts.Comment: 26 pages, 17 figure

Impacts of Smooth Pigweed (Amaranthus hybridus) on Cover Crops in Southern Ontario

Amaranthus hybridus is a noxious weed in Ontario, with demonstrated allelopathic properties that can lead to decreased agricultural production. We tested the germination and growth of five cover crop species exposed to A. hybridus extracts, and to dried or fresh materials in soil. A germination index was calculated, and the dry weight of plant organs were measured to quantify responses to treatments. All species had reduced germination (≤29%) in 100% extract. Trifolium pratense had significant root weight reductions in extract (52%) and dried (72%) treatments, whereas shoot weight only decreased (48%) in dried treatment. Medicago sativa shoot weight decreased (52%) in 20g fresh treatment, while root weight decreased (62%) in dried treatment. Shoot weight of Raphanus sativus increased (32%) at mid-extract concentrations, while root weight increased (33%) only with dried treatment; however, both its shoot and root weight decreased (\u3e40%) in fresh treatment. Only the shoot weight of Lolium multiflorum increased (41% in 75% extract and 55% in dried treatment). Both Cichorium intybus shoot and root weights decreased (~50%) in fresh treatment. Crop responses to A. hybridus are complex, and material and species-dependant. Further testing in the field may provide a more comprehensive understanding of how to improve the management of A. hybridus

Genetic Control of Root Weight, Root Volume and Root to Shoot Weight Ratio in Peas

The genetic control of root weight, root volume and root to shoot weight ratio was studied in peas (Pisum sativum L.) at flowering. The four populations used were developed from crosses between lines and cultivars differing in size of the root systems. Plants of the F1, F2 , back cross and parental generations were evaluated in pots filled with soil: sand mixture, in a completely randomized design. A generation means analysis was used to determine the inheritance of the root characteristics. Root weight and volume exhibited moderate to high broad-sense heritability (0.41 - 0.81 for root weight, and 0.44 - 0.77 for root volume). Additive and dominance effects were important in the inheritance of root weight and volume in all populations, while epistatic effects were important only in two populations. The importance of the genetic parameters in the inheritance of root to shoot weight ratio was unclear. With the presence of large additive effects and high heritability, estimates for root weight and volume, selection for superior pure lines with large root systems should be effective