Seed production, infestation, and viability in Acacia tortilis (synonym: Vachellia tortilis) and Acacia robusta (synonym: Vachellia robusta) across the Serengeti rainfall gradient

Tree recruitment in savannas proceeds in multiple stages characterized by successive filters occurring at the seed and seedling stages. The “demographic bottleneck” hypothesis suggests that such filters ultimately restrict tree density and prevent trees from dominating grasses in savannas, but many of the demographic transitions underlying this assumption have not been quantified. We investigated how short- (1–2 years) and long-term (40 + years) rainfall patterns influenced seed production, infestation, and viability for two dominant species, Acacia robusta and Acacia tortilis across the Serengeti ecosystem mean annual precipitation gradient over a two-year period. We found that neither production, nor infestation, nor viability was influenced by rainfall. Pod production differed between species and increased with tree height in A. robusta. Mean infestation proportion in 2013 was higher (mean ± SE; 0.28 ± 0.08) in A. tortilis than in A. robusta (0.11 ± 0.05) but the trend reversed in 2014, when A. tortilis (0.33 ± 0.10) had lower infestation than A. robusta (0.61 ± 0.09). Under laboratory conditions, A. tortilis and A. robusta seeds had maximum germination (= viability) proportions of 70 and 20%, respectively. Mean seed viability was more than five-fold higher (0.46 ± 0.19) in A. tortilis than in A. robusta (0.08 ± 0.10). Our study has produced important estimates for seed stage demographic dynamics that can be used for modeling tree dynamics in Serengeti system, and savannas in general

Taxonomic status of Bambusaspis miliaris : B. robusta, and B. pseudomiliaris (Hemiptera: Coccoidea: Asterolecaniidae)

Based on an assessment of 50 morphological characters from 110 specimens of Bambusaspis miliaris (BoisduvalI869), B. robusta (Green 1908), andB. pseudomiliaris (Green 1922) from different geographic regions around the world, we conclude that these specimens represent the same species. Therefore, the taxa B. robusta and B. pseudomiliaris are considered junior subjective synonyms of B. miliaris

Morphological separation of the Central European Trochosa females (Araneae, Lycosidae)

Adult females of the five Central European wolf spiders Trochosa hispanica Simon, 1870, T. robusta (Simon, 1876), T. ruricola (De Geer, 1778), T. spinipalpis (F. O. P.-Cambridge, 1895), and T. terricola Thorell, 1856 were morphologically analysed. We defined sets of continuous and binary (presence/absence) variables. Continuous data of various epigynal and carapace dimensions were subjected to Principal Components Analysis (PCA). Using the PC loadings each individual was plotted along the PC axis in order to find gaps/overlaps between the species. The binary data sets were subjected to Hierarchical Cluster Analysis (HCA) in order to find characters that clearly separate the five Trochosa species. Using PCA only individuals of T. robusta and T. ruricola and of T. robusta and T. hispanica could be separated from each other. Using HCA all five species could clearly be separated by epigynal and vulval characteristics

Carbon assessment for Robusta coffee production systems in Vietnam: a case study in Dak Lak

Carbon assessments have proliferated to identify climate friendly practices in Arabica producing systems, though little attention is given to Robusta. In this study, we evaluated the climate impact of Robusta production via quantification of carbon stock and greenhouse gas (GHG) emissions in the intensive shaded and unshaded coffee farms of the world’s largest Robusta producing region, Vietnam’s Central Highlands. We find due to the linear relationship between fertilizer use and yields, emissions from input use on a per unit product basis are not significantly different between the intensive and less intensive systems. However, when accounting for carbon sequestered in shade and coffee trees, the less intensive systems are carbon positive (sequestering more than they emit) per unit of green coffee bean produced

MEAT QUALITY OF THREE CHICKEN GENOTYPES REARED ACCORDING TO THE ORGANIC SYSTEM

The meat quality of three poultry genotypes with differing growth rates (fast-growing Ross; medium and slow growing Kabir and Robusta maculata, respectively) was compared. All the birds were reared according to the organic production system which requires a paddock with grass pasture (4 m2/bird) and a slaughter age greater than 81 d. The trial was carried out on 100 female chickens per strain. The meat quality was affected by the 20 different degree of maturity of the strains at slaughter age, which was 70% for Ross, 52 % for Kabir and 78% for Robusta maculata. Ross and Kabir were slaughtered at 81 d, whereas Robusta maculata, required 120 d to reach a commercial weight (>2 kg). The meat of all the three genotypes showed good qualitative traits. The main differences of the three genotypes regarded moisture, lipid, pHu, colour, iron, oxidative stability and overall acceptance. Compared with Kabir and Robusta maculata Ross meat had more fat, lower pHu and iron, and was 25 paler. The oxidative stability during display (24-96 h at 4° C) and acceptance were the worst. Kabir chickens, being the least mature strain, had the highest moisture content with a high cooking loss. The slower-growing genotypes showed a good adaptation to the extensive rearing conditions, while the fast-growing genotype showed unbalanced muscle response to the greater activity and the oxidative stability of the meat was reduced

Evaluation of Somatic Embryogenesis Ability in Robusta Coffee (Coffea Canephora Pierre)

Embriogenesis somatik diharapkan sebagai metode perbanyakan tanaman yang sangat efektif pada kopi. Evaluasi dua jenis proses embriogenesis somatik, yaitu proses langsung dan tidak langsung akan bermanfaat untuk menggambarkan kemampuan proliferasi sel. Penelitian untuk mengevaluasi embriogenesis somatik kopi Robusta (Coffea canephora) yang mempunyai tingkat keragaman genetik tinggi telah dilakukan di Nestlé R&D Centre Tours, Perancis. Bahan tanam menggunakan kopi Robusta koleksi Nestle Perancis dan tiga klon koleksi Pusat Penelitian Kopi dan Kakao Indonesia (Puslitkoka). Tiga aspek, yaitu proses embriogenesis, keragaman embriogenesis dan kemantapan embriogenesis dievaluasi dalam penelitian ini. Hasil penelitian menunjukkan bahwa baik embriogenesis somatik langsung maupun tidak langsung dapat diamati. Penelitian ini menunjukkan bahwa kedua proses embriogenesis somatik tersebut merupakan dua mekanisme yang berbeda. Dalam penelitian ini ditunjukkan bahwa kemampuan embriognesis somatik tergantung pada genotipe, baik antar maupun di dalam kelompok genetik kopi Robusta, yaitu Congolese,Guinean dan Conillon. Lebih lanjut diketahui bahwa kedua proses embriogenesis somatik tersebut stabil terhadap indukan sebagai sumber eksplan. Kemampuan embriogenesis somatik tidak langsung ketiga klon Puslitkoka (BP409, BP961 dan Q121) sangat beragam, sehingga memberikan harapan adanya pola segregasi yang baik berdasarkan kemampuan embriogenesis somatik tidak langsung pada populasi yang dibuat dari silangan klon tersebut

Associated bacteria affect sexual reproduction by altering gene expression and metabolic processes in a biofilm inhabiting diatom

Diatoms are unicellular algae with a fundamental role in global biogeochemical cycles as major primary producers at the base of aquatic food webs. In recent years, chemical communication between diatoms and associated bacteria has emerged as a key factor in diatom ecology, spurred by conceptual and technological advancements to study the mechanisms underlying these interactions. Here, we use a combination of physiological, transcriptomic, and metabolomic approaches to study the influence of naturally coexisting bacteria, Maribacter sp. and Roseovarius sp., on the sexual reproduction of the biofilm inhabiting marine pennate diatom Seminavis robusta. While Maribacter sp. severely reduces the reproductive success of S. robusta cultures, Roseovarius sp. slightly enhances it. Contrary to our expectation, we demonstrate that the effect of the bacterial exudates is not caused by altered cell-cycle regulation prior to the switch to meiosis. Instead, Maribacter sp. exudates cause a reduced production of diproline, the sexual attraction pheromone of S. robusta. Transcriptomic analyses show that this is likely an indirect consequence of altered intracellular metabolic fluxes in the diatom, especially those related to amino acid biosynthesis, oxidative stress response, and biosynthesis of defense molecules. This study provides the first insights into the influence of bacteria on diatom sexual reproduction and adds a new dimension to the complexity of a still understudied phenomenon in natural diatom populations

PERFORMANCE AND BEHAVIOUR OF CHICKENS WITH DIFFERENT GROWING RATE REARED ACCORDING TO THE ORGANIC SYSTEM

The performance and the behaviour of three different chicken strains, reared according to the EEC-Regulation 1804/1999 organic system, were compared. The strains had very slow (Robusta maculata), slow (Kabir) and fast (Ross) growing rates, respectively. The trial was carried out on 200 chickens (male and female) per strain. Rearing lasted 81 days as required by the EEC Regulations. At slaughter age, 20 birds per group were killed. Robusta maculata and Kabir chickens showed more intense walking activity and better foraging aptitude; their antioxidant capacity was also superior. Ross chickens had a good growth rate and feed conversion index, reaching an excellent body weight, but the mortality and the culling rate were high indicating that fast-growing strains do not adapt well to organic production. Robusta maculata showed the worst productive performance although the mortality was low and Kabir birds gave intermediate results. The carcass traits were the best in Ross and the poorest in Robusta maculata. Male chickens were heavier and leaner than females

ANALISIS FAKTOR-FAKTOR PRODUKSI YANG MEMPENGARUHI PRODUKSI KOPI ROBUSTA DI KECAMATAN SUMOWONO KABUPATEN SEMARANG

Penelitian bertujuan untuk menganalisis jumlah produksi rata-rata kopi Robusta petani di Kecamatan Sumowono, menganalisis faktor-faktor yang berpengaruh terhadap produksi kopi Robusta di Kecamatan Sumowono, serta untuk mengetahui faktor yang paling berpengaruh terhadap produksi kopi Robusta di Kecamatan Sumowono. Lokasi penelitian ditentukan secara purposive. Metode yang digunakan adalah metode survei dan pemilihan sampel ditentukan dengan memilih 25 responden setiap 3 desa di Kecamatan Sumowono, sehingga berjumlah 75 responden. Hasil penelitian menunjukan bahwa produksi rata-rata kopi Robusta petani di Kecamatan Sumowono sebesar 1.617 kg/ha lebih tinggi dari produksi rata-rata kopi Robusta petani di provinsi Jawa Tengah sebesar 1.200 kg/ha, dan Nasional yaitu sebesar 855 kg/ha. Secara serempak luas lahan, jumlah pohon, jumlah pupuk kandang, jumlah pupuk NPK dan penggunaan tenaga kerja secara serempak berpengaruh nyata terhadap produksi kopi Robusta, sedangkan secara parsial hanya jumlah, pupuk kandang dan tenaga kerja yang berpengaruh nyata terhadap produksi kopi Robusta. Tenaga kerja adalah faktor yang paling berpengaruh terhadap faktor produksi kopi Robusta di Kecamatan Sumowono