FORMULATION AND PHYSICOCHEMICAL STABILITY OF 20% GLYCOLIC ACID CREAMS USING POLYACRYLAMIDE, C13-14 ISOPARAFFIN, LAURETH-7 AND COMBINATION OF POLYACRYLAMIDE, C13-14 ISOPARAFFIN, LAURETH 7 WITH A GLUKOLIPID OF VEGETABLE ORIGIN

Glycolic acid is a α-hydroxy acids (AHAs). It are used in skin lightening creams. It were developed formulation of 20% glycolic acid creams. The creams were made using 4% of polyacrylamide, C13-14 isoparaffin, laureth-7 as a thickening agent for cream gels and emulsions base and combination of 1.5% of polyacrylamide, C13-14 isoparaffin, laureth-7 and 5% of a glukolipid of vegetable origin as emulisufier agent. Then the physicochemical stabilities were tested using climatic chamber for 30 days at 40˚C with 75% Relative Humidity (RH). The parameters stability observed were organoleptic, droplet size, density, viscosity, emulsion type, phase separation, pH and concentration of acid glikolat.Organoleptis, droplet size, density, viscosity, type of emulsion and phase separation of all of creams were stable, but the pH decreased during the storage time. The time of the concentration of glycolic acid remaining to 90% in cream using using 4% of polyacrylamide, C13-14 isoparaffin, laureth-7 and using combination of using 4% of polyacrylamide, C13-14 isoparaffin, laureth-7 with 5% of a glukolipid of vegetable origin were 30 and 32 days respectively

A Study of Dual Polymer Retention Aids for the Retention on Titanium Dioxide Using the Dynamic Drainage Jar, Minidrinier, and Handsheets

Two types of dual polymer retention aid systems, a low molecular weight, high charge density, cationic polyamine with a high molecular weight, low charge density, cationic polyacrylamide, and the same polyamine with a high molecular weight, highly charged anionic polyacrylamide, were studied using the Dynamic Drainage Jar, the Minidrinier, and handsheets. Both systems gave higher retention than could be achieved using any of the retention aids alone, however, formation was a problem. Contact time and shear were shown to be important variables. All three testing methods were useful, the Dynamic Drainage Jar having an advantage due to its flexibility

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE

A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC-PAGE

Interaction of iron oxide nanoparticles synthesized by laser target evaporation with polyacrylamide in composites and ferrogels

Received: 05.06.2017; accepted: 20.06.2017;published: 14.07.2017.Iron oxide magnetic nanoparticles (MNPs) with average diameter 11.7 nm synthesized by laser target evaporation were used for the synthesis of composites and ferrogels based on polyacrylamide network. The chemical composition of MNPs corresponded to maghemite. It was shown that intact MNPs strongly interacted with polyacrylamide polymeric network, while the adsorption of electrostatic stabilizer on the surface of MNPs efficiently prevents such interaction. Synthesis of ferrogels was performed by the radical polymerization of acrylamide in electrostatically stabilized suspensions of MNPs in water. It was shown that the molecular structure, water uptake, and compression modulus can be controlled by the concentration of monomer taken in the synthesis

Conjugatable water-soluble Pt(ii) and Pd(ii) porphyrin complexes: Novel nano- and molecular probes for optical oxygen tension measurement in tissue engineering

Measurement of oxygen tension in compressed collagen sheets was performed using matrix-embedded optical oxygen sensors based on platinum(II) and palladium(II) porphyrins supported on polyacrylamide nanoparticles. Bespoke, fully water-soluble, mono-functionalised Pt(II) and Pd(II) porphyrin complexes designed for conjugation under mild conditions were obtained using microwave-assisted metallation. The new sensors display a linear response (1/τ vs. O₂) to varying oxygen tension over a biologically relevant range (7.0 × 10⁻⁴ to 2.7 × 10⁻¹ mM) in aqueous solutions; a behaviour that is maintained following conjugation to polyacrylamide nanoparticles, and following embedding of the nanosensors in compressed collagen sheets, paving the way to innovative approaches for real-time resolution of oxygen gradients throughout 3D matrices useful for tissue regeneration

The electrophoresis of transferrins in urea/polyacrylamide gels

The denaturation of transferrin by urea has been studied by (a) electrophoresis in polyacrylamide gels incorporating a urea gradient, (b) measurements of the loss in iron-binding capacity and (c) u.v. difference spectrometry. In human serum transferrin and hen ovotransferrin the N-terminal and C-terminal domains of the iron-free protein were found to denature at different urea concentrations

Characterization of the Lipopolysaccharide from a \u3cem\u3eRhizobium phaseoli\u3c/em\u3e Mutant that is Defective in Infection Thread Development

The lipopolysaccharide (LPS) from a Rhizobium phaseoli mutant, CE109, was isolated and compared with that of its wild-type parent, CE3. A previous report has shown that the mutant is defective in infection thread development, and sodium dodecyl sulfate-polyacrylamide gel electrophoresis shows that it has an altered LPS (K. D. Noel, K. A. VandenBosch, and B. Kulpaca, J. Bacteriol. 168:1392-1462, 1986). Mild acid hydrolysis of the CE3 LPS released a polysaccharide and an oligosaccharide, PS1 and PS2, respectively. Mild acid hydrolysis of CE109 LPS released only an oligosaccharide. Chemical and immunochemical analyses showed that CE3-PS1 is the antigenic O chain of this strain and that CE109 LPS does not contain any of the major sugar components of CE3-PS1. CE109 oligosaccharide was identical in composition to CE3-PS2. The lipid A\u27s from both strains were very similar in composition, with only minor quantitative variations. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis of CE3 and CE109 LPSs showed that CE3 LPS separated into two bands, LPS I and LPS II, while CE109 had two bands which migrated to positions similar to that of LPS II. Immunoblotting with anti-CE3 antiserum showed that LPS I contains the antigenic O chain of CE3, PS1. Anti-CE109 antiserum interacted strongly with both CE109 LPS bands and CE3 LPS II and interacted weakly with CE3 LPS I. Mild-acid hydrolysis of CE3 LPS I, extracted from the polyacrylamide gel, showed that it contained both PS1 and PS2. The results in this report showed that CE109 LPS consists of only the lipid A core and is missing the antigenic O chain

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE)

QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point. This variant of gel electrophoresis is used by biologists to isolate active or native metalloproteins in biological samples and to resolve properly and improperly folded metal cofactor-containing proteins in complex protein mixtures