Localisation of RNAs into the germ plasm of vitellogenic xenopus oocytes

We have studied the localisation of mRNAs in full-grown Xenopus laevis oocytes by injecting fluorescent RNAs, followed by confocal microscopy of the oocyte cortex. Concentrating on RNA encoding the Xenopus Nanos homologue, nanos1 (formerly Xcat2), we find that it consistently localised into aggregated germ plasm ribonucleoprotein (RNP) particles, independently of cytoskeletal integrity. This implies that a diffusion/entrapment-mediated mechanism is active, as previously reported for previtellogenic oocytes. Sometimes this was accompanied by localisation into scattered particles of the “late”, Vg1/VegT pathway; occasionally only late pathway localisation was seen. The Xpat RNA behaved in an identical fashion and for neither RNA was the localisation changed by any culture conditions tested. The identity of the labelled RNP aggregates as definitive germ plasm was confirmed by their inclusion of abundant mitochondria and co-localisation with the germ plasm protein Hermes. Further, the nanos1/Hermes RNP particles are interspersed with those containing the germ plasm protein Xpat. These aggregates may be followed into the germ plasm of unfertilized eggs, but with a notable reduction in its quantity, both in terms of injected molecules and endogenous structures. Our results conflict with previous reports that there is no RNA localisation in large oocytes, and that during mid-oogenesis even germ plasm RNAs localise exclusively by the late pathway. We find that in mid oogenesis nanos1 RNA also localises to germ plasm but also by the late pathway. Late pathway RNAs, Vg1 and VegT, also may localise into germ plasm. Our results support the view that mechanistically the two modes of localisation are extremely similar, and that in an injection experiment RNAs might utilise either pathway, the distinction in fates being very subtle and subject to variation. We discuss these results in relation to their biological significance and the results of others

On the conical refraction of hydromagnetic waves in plasma with anisotropic thermal pressure

A phenomenon analogous to the conical refraction widely known in the crystalooptics and crystaloacoustics is discovered for the magnetohydrodynamical waves in the collisionless plasma with anisotropic thermal pressure. Angle of the conical refraction is calculated for the medium under study which is predicted to be $18^{\circ}26^{\prime}$. Possible experimental corroborating of the discovered phenomenon is discussed.Comment: 6 pages, REVTeX, Accepted in Physics of Plasma

Protein interactions in Xenopus germ plasm RNP particles

Hermes is an RNA-binding protein that we have previously reported to be found in the ribonucleoprotein (RNP) particles of Xenopus germ plasm, where it is associated with various RNAs, including that encoding the germ line determinant Nanos1. To further define the composition of these RNPs, we performed a screen for Hermes-binding partners using the yeast two-hybrid system. We have identified and validated four proteins that interact with Hermes in germ plasm: two isoforms of Xvelo1 (a homologue of zebrafish Bucky ball) and Rbm24b and Rbm42b, both RNA-binding proteins containing the RRM motif. GFP-Xvelo fusion proteins and their endogenous counterparts, identified with antisera, were found to localize with Hermes in the germ plasm particles of large oocytes and eggs. Only the larger Xvelo isoform was naturally found in the Balbiani body of previtellogenic oocytes. Bimolecular fluorescence complementation (BiFC) experiments confirmed that Hermes and the Xvelo variants interact in germ plasm, as do Rbm24b and 42b. Depletion of the shorter Xvelo variant with antisense oligonucleotides caused a decrease in the size of germ plasm aggregates and loosening of associated mitochondria from these structures. This suggests that the short Xvelo variant, or less likely its RNA, has a role in organizing and maintaining the integrity of germ plasm in Xenopus oocytes. While GFP fusion proteins for Rbm24b and 42b did not localize into germ plasm as specifically as Hermes or Xvelo, BiFC analysis indicated that both interact with Hermes in germ plasm RNPs. They are very stable in the face of RNA depletion, but additive effects of combinations of antisense oligos suggest they may have a role in germ plasm structure and may influence the ability of Hermes protein to effectively enter RNP particles

Theory of the Spatio-Temporal Dynamics of Transport Bifurcations

The development and time evolution of a transport barrier in a magnetically confined plasma with non-monotonic, nonlinear dependence of the anomalous flux on mean gradients is analyzed. Upon consideration of both the spatial inhomogeneity and the gradient nonlinearity of the transport coefficient, we find that the transition develops as a bifurcation front with radially propagating discontinuity in local gradient. The spatial location of the transport barrier as a function of input flux is calculated. The analysis indicates that for powers slightly above threshold, the barrier location $x_b(t) \sim ( D_n t (P-P_c)/P_c)^{1/2},$ where $P_c$ is the local transition power threshold and $D_n$ is the neoclassical diffusivity . This result suggests a simple explanation of the high disruptivity observed in reversed shear plasmas. The basic conclusions of this theory are insensitive to the details of the local transport model.Comment: 21 page Tex file, 10 postscript file

A Review of Recent Developments in Atomic Processes for Divertors and Edge Plasmas

The most promising concepts for power and particle control in tokamaks and other fusion experiments rely upon atomic processes to transfer the power and momentum from the edge plasma to the plasma chamber walls. This places a new emphasis on processes at low temperatures (1-200 eV) and high densities (10^20-10^22 m^-3). The most important atomic processes are impurity and hydrogen radiation, ionization, excitation, recombination, charge exchange, radiation transport, molecular collisions, and elastic scattering of atoms, molecules and ions. Important new developments have occurred in each of these areas. The best available data for these processes and an assessment of their role in plasma wall interactions are summarized, and the major areas where improved data are needed are reviewed.Comment: Preprint for the 11th PSI meeting, postscript with 22 figures, 40 page

Magnetohydrodynamic equilibria of a cylindrical plasma with poloidal mass flow and arbitrary cross section shape

The equilibrium of a cylindrical plasma with purely poloidal mass flow and cross section of arbitrary shape is investigated within the framework of the ideal MHD theory. For the system under consideration it is shown that only incompressible flows are possible and, conscequently, the general two dimensional flow equilibrium equations reduce to a single second-order quasilinear partial differential equation for the poloidal magnetic flux function $\psi$, in which four profile functionals of $\psi$ appear. Apart from a singularity occuring when the modulus of Mach number associated with the Alfv\'en velocity for the poloidal magnetic field is unity, this equation is always elliptic and permits the construction of several classes of analytic solutions. Specific exact equlibria for a plasma confined within a perfectly conducting circular cylindrical boundary and having i) a flat current density and ii) a peaked current density are obtained and studied.Comment: Accepted to Plasma Physics & Controlled Fusion, 14 pages, revte

Apparatus for measuring conductivity and velocity of plasma utilizing a plurality of sensing coils positioned in the plasma Patent

Apparatus for measuring conductivity and velocity of plasma with multiple sensing coils positioned in plasm