Developments in plant breeding for improved nutritional quality of soya beans II. Anti-nutritional factors

Nutritional value of most plant materials is limited by the presence of numerous naturally occurring compounds which interfere with nutrient digestion and absorption. Although processing is employed widely in removal of these factors, selection of cultivars of soya beans with inherently low levels would have a considerable impact on efficiency of non-ruminant livestock production. The review considers the role of plant breeding in achieving this objective. The most abundant trypsin inhibitors are the Kunitz and the Bowman Birk inhibitors, containing 181 and 71 amino acids respectively. The Kunitz inhibitor is present at a concentration of 1.4g/kg of total seed contents and the Bowman Birk inhibitor 1.6g/kg. A large number of isoforms of the Bowman Birk inhibitor have been described in soya bean cultivars and it has been shown that the general properties of the inhibitor are, in fact, attributable to different isoforms. Nulls for both Bowman-Birk and Kunitz trypsin inhibitors have been identified, allowing new low trypsin inhibitor cultivars to be produced. However, research into breeding for low trypsin inhibitor cultivars currently has limited application as trypsin inhibitors contribute a major proportion of the methionine content of soya beans. Trypsin inhibitors are thought to be involved in the regulation of and protection against unwanted proteolysis in plant tissues and also act as a defense mechanism against attack from diseases, insects and animals. Hence, in breeding programes for low trypsin inhibitor cultivars, alternative protection for growing plants must be considered. Use of soya beans in non-ruminant animal feeds is limited by the flatulence associated with their consumption

Application of Co-bioprocessing Techniques (Enzymatic Hydrolysis and Fermantation) for Improving the Nutritional Value of Wheat Bran as Food Functional Ingrediens

Last time the food industry pays the great attention to questions, connected with changing existing technologies for raising the efficacy of the raw materials complex processing and increasing the output of high-quality products and food ingredients with a minimal amount of waste. Cereal crops are the most reach source of functional ingredients and main component in the human food ration. The technological process of cereal crops processing at enterprises is closely connected with creating a great number of secondary raw material resources and its further utilization.For confirming the efficacy of using secondary products of grain processing as cheap raw material resources of dietary fiber and physiologically functional ingredients, there is characterized the accessibility of their biotransformation that gives a possibility to get biologically active substances of different chemical nature with a wide spectrum of physiological effects.Secondary products of cereal crops processing (bran) are multi-component substrates, formed of different histological layers of wheat grains after comminution, consisted of (external pericarp, internal pericarp, grain coat, hyaline and aleurone layer of a grain coat).Wheat bran is rich in dietary fiber, nutritive and phytochemical substances, that is why, it is most often used for feeding animals. But for today there are important proofs of using it in the food industry.The development of new innovative technologies, modern achievements in microbiology and biotechnology have an important value for secondary products of grain processing, because they allow to conduct directed technological processes at the qualitatively new level that provides using soft regimes of vegetable raw materials processing, allowing to preserve natural biologically active substances and nutrients.The modeling of the combined complex processing that includes enzymatic hydrolysis and fermentation by microorganisms improves technological, sensor and also nutritive and physiologically functional properties of wheat bran at the expanse of: bioavailability increase of phenol compounds, vitamins and minerals, assimilability of proteins and decrease of the content of anti-nutritive compounds.Enzymatic preparations allow to use vegetable raw materials rationally, to intensify technological processes, in such a way increasing the output of biologically active substances and to widen the assortment of created products. The process of wheat bran formation results in increasing the nutritional value, enriching the biopolymeric complex with probiotic microorganisms and prebiotic substances.Based on the structural peculiarities and multicomponent composition of wheat bran, presented and studied in the article, it has been established, that the use of the directed modification allows to get functional ingredients and products with set properties that influence the human health favorably. So, wheat bran must be used not only in agriculture as a cattle fodder, but also in the food industry

Citric Acid and Microbial Phytase Inclusion in the Diet to Improve Utilization Phytate Phosporus and Growth of Broiler

Penelitian ini menggunakan anak ayam umur 3 sampai dengan 38 hari (180 ekor) dan 7 sampai dengan 42 hari (180 ekor). Ayam diberi ransum A, B, dan C yang berbahan dasar jagung (CSM) dan ransum E, F, dan G yang berbahan dasar gandum (WSM) yang diberi perlakuan penambahan steam pada suhu 1000C selama 10 menit. Semua ransum ditambah enzim fitase (SP-1002 ct) sebanyak 500 U/kg dan dibuat dalam bentuk pelet. Khusus ransum C dan G ditambah asam sitrat. Kandungan fosfor total ransum adalah 4,5 g/kg dan fosfor tersedia adalah 1,5 g/kg (defisien P). Bobot badan harian adalah 36, 34, dan 45 g/hari untuk ayam yang diberi ransum A, B, dan C dan 54, 46, dan 55 g/hari untuk ransum E, F, dan G. Deposisi fosfor total adalah 5,75; 5,36; dan 6,99 g berturut-turut untuk ransum A, B dan C dan 8,15; 6,90; dan 7,77 g untuk E, F, dan G. Penambahan asam sitrat dalam ransum CSM yang mengandung enzim fitase rendah mampu meningkatkan pertumbuhan dan deposisi fosfor. Pada ransum WSM, perlakuan panas menyebabkan penurunan aktifitas fitase dan penurunan ini tidak dapat digantikan dengan penambahan enzim fitase (microbial phytase) akan tetapi dapat digantikan dengan penambahan asam sitrat. Hasil penelitian ini dapat disimpulkan bahwa asam sitrat berpengaruh terhadap efisiensi enzim fitase (microbial phytase) dalam mendegradasi senyawa fitat di dalam saluran pencernaan

Phytase: application in food industry

Phytates have been considered as a threat in human diet due to its antinutrients behaviour which known as strong chelators of divalent minerals such as Ca²+, Mg²+, Zn²+ and Fe²+. Phytic acid has a potential for binding positively charged proteins, amino acids, and/or multivalent cations or minerals in foods. The resulting complexes are insoluble, difficult for humans to hydrolyze during digestion, and thus, typically are nutritionally less available for absorption. The reduction of this phytates can be achieved through both enzymatic and non-enzymatic removal. Enzymatic degradation includes addition of either isolated form of wild-type or recombinant exogenous phytate-degrading enzymes microorganisms in the food matrix. Non-enzymatic hydrolysis of phytate occurred in the final food during food processing or physical separation of phytate-rich parts of the plants seed. The application of phytase with respect to breadmaking process, probiotics, animal feed supplement and transgenic crops are emphasised in this paper

Bioavailability of zinc sources and their interaction with phytates in broilers and piglets

Zinc (Zn) is essential for swine and poultry and native Zn concentrations in feedstuffs are too low to meet their Zn requirement. Dietary Zn bioavailability is affected by phytate, phytase and Zn supplemented in organic form is considered as more bioavailable than inorganic sources. A meta-analysis using GLM procedures was processed using broiler and piglet databases to investigate, within the physiological response of Zn, (1) the bioavailability of inorganic and organic Zn sources (Analysis I); (2) the bioavailability of native and inorganic Zn dependent from dietary phytates, vegetal and supplemental phytase activity (Analysis II). Analysis I: the bioavailability of organic Zn relative to inorganic Zn sources ranged, depending on the variable, from 85 to 117 never different from 100 (P > 0.05). The coefficients of determination of the regressions were 0.91 in broilers and above 0.89 in piglets. Analysis II: in broilers, bone Zn was explained by supplemental Zn (linear and quadratic, P 0.05). The coefficients of determination of the regressions were 0.92 in broilers and above 0.92 in piglets. The results from the two meta-analyses suggest that (1) broilers and piglets use supplemented Zn, independent from Zn source; (2) broiler use native Zn and the use is slightly enhanced with supplemental phytase; (3) however, piglets are limited in the use of native Zn because of the antagonism of non-hydrolyzed dietary phytate. This explains the higher efficacy of phytase in improving Zn availability in this speci

Biodisponibilidade do cálcio do grão de amaranto antes e após extrusão

Calcium bioavailability of raw and extruded amaranth grains was assessed in a biological assay in rats. Rats were fed for 28 days on diets in which raw or extruded amaranth was the only calcium source, compared to a control diet with calcium carbonate. Calcium and phosphorous levels were determined in the rats' serum during the experimental period and in the bones at the end of the experiment. Amaranth extrusion increased its calcium bioavailability, assessed by tibia and femur weights and calcium and phosphorous content of the bones. Apparent calcium absorption index, the force needed to break the bones and bone densitometry of both extruded and raw amaranth were the same, though different from the control group. The results show that amaranth can be a complementary source of dietary calcium the bioavailability of which is favorably modified by the extrusion process.A biodisponibilidade do cálcio de amaranto antes e após extrusão foi avaliada em ensaio biológico. Ratos, alimentados por 28 dias com dietas em que amaranto, antes ou após extrusão, era a única fonte de cálcio da dieta, foram comparados com animais em dieta controle com teor próximo de cálcio, oferecido na forma de carbonato de cálcio. Cálcio e fósforo foram determinados no soro dos animais durante o período experimental e nos ossos, ao final do experimento. A extrusão do amaranto aumentou a biodisponibilidade do seu cálcio, quando avaliado pelo conteúdo de cálcio e fósforo da tíbia e fêmur dos animais, bem como pelo peso desses ossos. O índice aparente de absorção de cálcio, força para romper os ossos e densidade óssea foram iguais antes e após a extrusão do amaranto e diferentes do grupo controle. Os resultados mostram que o amaranto pode ser uma fonte complementar de cálcio e que sua biodisponibilidade aumenta com o processo de extrusão.(CAPES) Coordenação do Aperfeiçoamento de Pessoal de Nível Superior - PICDT ProgramFAPES

Iron Bioavailability and Provitamin A from Sweet Potato- and Cereal-Based Complementary Foods

Iron and vitamin A deficiencies in childhood are public health problems in the developing world. Introduction of cereal-based complementary foods, that are often poor sources of both vitamin A and bioavailable iron, increases the risk of deficiency in young children. Alternative foods with higher levels of vitamin A and bioavailable iron could help alleviate these micronutrient deficiencies. The objective of this study was to compare iron bioavailability of β-carotene-rich sweet potato-based complementary foods (orange-flesh based sweet potato (OFSP) ComFa and cream-flesh sweet potato based (CFSP) ComFa with a household cereal-based complementary food (Weanimix) and a commercial cereal (Cerelac®), using the in vitro digestion/Caco-2 cell model. Iron bioavailability relative to total iron, concentrations of iron-uptake inhibitors (fibre, phytates, and polyphenols), and enhancers (ascorbic acid, ß-carotene and fructose) was also evaluated. All foods contained similar amounts of iron, but bioavailability varied: Cerelac® had the highest, followed by OFSP ComFa and Weanimix, which had equivalent bioavailable iron; CFSP ComFa had the lowest bioavailability. The high iron bioavailability from Cerelac® was associated with the highest levels of ascorbic acid, and the lowest levels of inhibitors; polyphenols appeared to limit sweet potato-based food iron bioavailability. Taken together, the results do not support that CFSP- and OFSP ComFa are better sources of bioavailable iron compared with non-commercial/household cereal-based weaning foods; however, they may be a good source of provitamin A in the form of β-carotene

Growth response of Heterobranchus longifilis (Valenciennes, 1840) fingerlings fed raw and boiled Mucuna cochinchinensis seed meal

Feeding trial was conducted in static water to assess the growth of H. longifilis fingerlings fed different I inclusion levels of mucuna seed meal (MSM). Raw and boiled MSM were used in the diets at 10%, 20%, 30% and 40% inclusion levels and the performance of fish fed these diets was compared with fish fed a fishmeal-based diet which contained 40% protein. All diets were prepared to be isonitrogenous and isocaloric A two by five factorial experiment with three replicates using ten fish of average initial weight 1 .46g was carried out. Daily fish ration of five percent body weight was administered two times for eight weeks. The specific growth rate in diets 1 (control) and 6(10% boiled MSM) were similar and significantly (P<0.05) higher than the other dietary groups. The significantly lower growth performance of fish fed diets containing higher inclusion levels of both raw and boiled MSM might be due to incomplete elimination of the antinutritional factors present in MSM by boiling. Other methods of processing MSM to improve its nutritive value should be investigated. (7 page document

Germination: a means to improve the functionality of oat

The biochemical and physiological reactions of germination have long been utilised to produce barley malt for brewing and other purposes. Also some oat malt has been produced as raw-material of ale and stout production. The main goals of malting have been the degradation of grain storage components to soften the kernel structure, synthesis of amylolytic enzymes and production of nutrients for brewing yeast. Also flavour and colour attributes have been important. During the recent years interest has arisen also in the secondary metabolites produced during germination, which can have valuable health promoting properties and act as bioactive or functional compounds in foods. By using a tailored germination/malting process a desired combination of valuable properties may be obtained in germinating grains or seeds. All this requires knowledge and know-how of the germination process and the biochemistry behind it. This paper reviews the scientific knowledge about germination/malting of oat with special emphasis on changes in grain characteristics

Correlation of calcium and magnesium intakes to frequency of muscle cramps in female college athletes

Muscle cramps are involuntary, painful, sudden contractions of skeletal muscles that can cause detrimental effects on athletic performance. Recent data suggest that low intakes of dietary calcium (Ca) and magnesium (Mg) can enhance or cause muscle cramps. The purpose of this study was to determine the correlation between Ca and Mg intakes and muscle cramping in female college athletes. Athletes completed a 24-hour dietary recall and a survey on frequency and location of muscle cramps. Of those surveyed, 8 participated in basketball, 21 in softball, and 10 in gymnastics. Calcium and Mg intakes were calculated from dietary recall data using Food Processor® nutrient analysis software. Data are reported as means ± standard deviation (SD). T-tests were performed to determine significant differences between groups. The average daily intakes of Ca above and below the dietary reference intake (DRI) were 1516 ± 559 mg/day and 504 ± 296 mg/day, respectively, for the athletes that cramped and 1620 ± 299 mg/day and 645 ± 250 mg/day, respectively, for the athletes that did not experience cramping. The average daily intakes above and below the DRI for Mg in the cramping group were 423 ± 103 mg/day and 180 ± 65 mg/day, respectively. The average daily intakes for those consuming Mg above and below the DRI in the non-cramping group were 476 ± 80 mg/day and 190 ± 64 mg/day, respectively. No significant differences in the intakes of Ca and Mg were found between groups that cramped and those that did not report cramping. The results of this study suggest that a high intake of Ca coupled with a low intake of Mg is positively correlated to muscle cramping