A study of cell membranes in nasal epithelial cells from patients with chronic rhinosinusitis with nasal polyps by means of a fluorescent probe

Aim. To assess the state of membranes in nasal epithelial cells obtained from the patients with chronic rhinosinusitis with nasal polyps (CRSwNP) with the help of the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole. Methods. The state of membrane phospholipid bilayer in suspensions of nasal epithelial cells isolated from ten patients with CRSwNP was evaluated using the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole that reacts on the physico-chemical properties of its microenvironment. Changes in fluorescence spectra were determined using a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher Scientific) 1 hour after the addition of the probe to nasal epithelial cell suspensions. Results. CRSwNP was found to be associated with a higher rate of nasal epithelial cell membrane hydration in the region of phospholipid glycerol moiety, carbonyl groups and aliphatic chains of fatty acids attached to the carbonyl groups. Conclusion. Our findings suggest that CRSwNP is accompanied by the elevated hydration rate of the most polar region, namely polar heads of phospholipids of nasal epithelial cell membranes

A study of cell membranes in nasal epithelial cells from patients with chronic rhinosinusitis with nasal polyps by means of a fluorescent probe

Aim. To assess the state of membranes in nasal epithelial cells obtained from the patients with chronic rhinosinusitis with nasal polyps (CRSwNP) with the help of the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole. Methods. The state of membrane phospholipid bilayer in suspensions of nasal epithelial cells isolated from ten patients with CRSwNP was evaluated using the fluorescent probe 2-(2ʹ-ОН-phenyl)-5-phenyl-1,3-oxazole that reacts on the physico-chemical properties of its microenvironment. Changes in fluorescence spectra were determined using a Thermo Scientific Lumina fluorescence spectrometer (Thermo Fisher Scientific) 1 hour after the addition of the probe to nasal epithelial cell suspensions. Results. CRSwNP was found to be associated with a higher rate of nasal epithelial cell membrane hydration in the region of phospholipid glycerol moiety, carbonyl groups and aliphatic chains of fatty acids attached to the carbonyl groups. Conclusion. Our findings suggest that CRSwNP is accompanied by the elevated hydration rate of the most polar region, namely polar heads of phospholipids of nasal epithelial cell membranes

Modelling of epitaxial graphene functionalization

A new model for graphene, epitaxially grown on silicon carbide is proposed. Density functional theory modelling of epitaxial graphene functionalization by hydrogen, fluorine and phenyl groups has been performed with hydrogen and fluorine showing a high probability of cluster formation in high adatom concentration. It has also been shown that the clusterization of fluorine adatoms provides midgap states in formation due to significant flat distortion of graphene. The functionalization of epitaxial graphene using larger species (methyl and phenyl groups) renders cluster formation impossible, due to the steric effect and results in uniform coverage with the energy gap opening.Comment: 15 pages, 4 figures, to appear in Nanotechnolog

Rotational barriers in perylene fluorescent dyes

Rotational barriers in N-substituted perylene dyes have been determined. Phenyl substituents with tert-butyl groups in the o-position give rigid systems, whereas secondary alkyl groups cause low rotational barriers. In spite of that, fluorescent quantum yields are high in both cases. Conformations in solution are discussed

Changes in Behavior, Movement, and Home Ranges of Largemouth Bass Following Large-scale Hydrilla Removal in Lake Seminole, Georgia

About 1,200 ha of hydrilla ( Hydrilla verticillata L.f. Royle) was eliminated in the Spring Creek embayment of Lake Seminole, Georgia, using a drip-delivery application of fluridone (1- methyl-3-phenyl-5-[3-(trifluoromethl) phenyl]-4(1H)-pyridinone) in 2000 and 2001. Two groups of 15 and 20 largemouth bass (Micropterus salmoides Lacepede) were implanted with 400-day radio tags in February 2000 and 2001 to determine changes in movement and behavior before and after hydrilla reduction.(PDF contains 8 pages.

Axial ligand binding equilibria as probes of the effective steric bulk of substituted tetraarylporphyrins

Equilibrium constants and thermodynamic data are reported for coordination of various axial ligands to the zinc(II) complexes of tetra( p-tolyl)porphyrin, tetra(3,5-bis(biphenylmethoxy)phenyl)porphyrin, tetra(2,6-(perfluorobenzoxy)phenyl)porphyrin, and tetra(2,6-di(methoxy)phenyl)porphyrin in toluene at 25.0°C. With respect to coordination of a planar ligand to a zinc(II) porphyrin, the equilibrium constant varied with the identity of the meso-substituent in the order 3,5-bis-(biphenylmethoxy)phenyl \u3e p-tolyl \u3e 2,6-(perfluorobenzoxy)phenyl \u3e\u3e 2,6-di-(methoxy)phenyl. With respect to coordination of a non-planar ligand to a zinc(II) porphyrin, the order was 3,5-bis(biphenylmethoxy)phenyl \u3e p-tolyl \u3e\u3e 2,6-(perfluorobenzoxy)phenyl ≈ 2,6-di(methoxy)phenyl. Thermodynamic data for the coordination of 3- and 4-phenylpyridine to zinc tetra(2,6-di(methoxy)phenyl) porphyrin show a greater loss of entropy than for the coordination of 3- and 4-phenylpyridine to zinc tetra (2,6-(perfluorobenzoxy)phenyl)porphyrin. Space-filling models show that the 2,6-dimethoxy groups of zinc tetra(2,6-di(methoxy)phenyl) porphyrin\u27s four-coordinate form have rotational degrees of freedom as opposed to the rotationally restricted 2,6-perfluorobenzoxy groups of zinc tetra(2,6-(perfluoro-benzoxy)phenyl)porphyrin\u27s four-coordinate form. The resulting loss of entropy due to coordination of an axial ligand (which restricts rotation of the meso-substituents) causes the 2,6-di(methoxy)phenyl groups to, in effect, be more sterically demanding than 2,6-(perfluorobenzoxy)phenyl groups in terms of lower axial ligation equilibrium constants. Also, experimental data suggest that 3,5-bis(biphenylmethoxy)phenyl groups at the meso-positions of a metalloporphyrin are no more sterically demanding than p-tolyl groups at the meso-positions

Multilayer screen gives cathode ray tube high contrast

Fabrication method for cathode ray tubes uses low-cost siloxane resin formulations. The resins contain sufficient methyl or phenyl groups for solubility in organic solvents. After vaporization and baking, the polymerized material is stable under vacuum and under temperatures required for tube fabrication

An \u3cem\u3em\u3c/em\u3e-Carboranedicarboxylic Acid Dianilide

The crystal structure of the \u27non hydrogen-bonded\u27 (according to IR data) polymorph of 1,7-bis(phenylcarbamoyl)-1,7-dicarba-closo-dodecaborane(12), C16-H22B10N2O2, has been determined. The two phenylamide groups have a Z configuration [the torsion angles 0-C-N-C are -2.3 (5) and -3.0 (5)°]. As a result both \u27active\u27 protons of these groups are almost completely shielded by other H atoms of the neighbouring carborane nucleus and phenyl substituents, and, therefore, no hydrogen-bonding contacts are found