Drug-induced Parkinson's disease modulates protein kinase A and Olfactory Marker Protein in the mouse olfactory bulb

Background Olfaction is often affected in parkinsonian patients, but dopaminergic cells in the olfactory bulb are not affected by some Parkinson-inducing drugs. We investigated whether the drug MPTP produces the olfactory deficits typical of Parkinson and affects the olfactory bulb in mice. Findings Lesioned and control mice were tested for olfactory search, for motor and exploratory behavior. Brains and olfactory mucosa were investigated via immunohistochemistry for thyrosine hydroxylase, Olfactory Marker Protein and cyclic AMP-dependent protein kinase as an intracellular pathway involved in dopaminergic neurotransmission. MPTP induced motor impairment, but no deficit in olfactory search. Thyrosine hydroxylase did not differ in olfactory bulb, while a strong decrease was detected in substantia nigra and tegmentum of MPTP mice. Olfactory Marker Protein decreased in the olfactory bulb of MPTP mice, while a cyclic AMP-dependent protein kinase increased in the inner granular layer of MPTP mice. Conclusions MPTP mice do not present behavioural deficits in olfactory search, yet immunoreactivity reveals modifications in the olfactory bulb, and suggests changes in intracellular signal processing, possibly linked to neuron survival after MPTP

Algorithms for Olfactory Search across Species

Localizing the sources of stimuli is essential. Most organisms cannot eat, mate, or escape without knowing where the relevant stimuli originate. For many, if not most, animals, olfaction plays an essential role in search. While microorganismal chemotaxis is relatively well understood, in larger animals the algorithms and mechanisms of olfactory search remain mysterious. In this symposium, we will present recent advances in our understanding of olfactory search in flies and rodents. Despite their different sizes and behaviors, both species must solve similar problems, including meeting the challenges of turbulent airflow, sampling the environment to optimize olfactory information, and incorporating odor information into broader navigational systems

Autonomous search for a diffusive source in an unknown environment

The paper presents an approach to olfactory search for a diffusive emitting source of tracer (e.g. aerosol, gas) in an environment with unknown map of randomly placed and shaped obstacles. The measurements of tracer concentration are sporadic, noisy and without directional information. The search domain is discretised and modelled by a finite two-dimensional lattice. The links is the lattice represent the traversable paths for emitted particles and for the searcher. A missing link in the lattice indicates a blocked paths, due to the walls or obstacles. The searcher must simultaneously estimate the source parameters, the map of the search domain and its own location within the map. The solution is formulated in the sequential Bayesian framework and implemented as a Rao-Blackwellised particle filter with information-driven motion control. The numerical results demonstrate the concept and its performance.Comment: 11 pages, 7 figure

Olfactory cue use by three-spined sticklebacks foraging in turbid water: prey detection or prey location?

Foraging, when senses are limited to olfaction, is composed of two distinct stages: the detection of prey and the location of prey. While specialist olfactory foragers are able to locate prey using olfactory cues alone, this may not be the case for foragers that rely primarily on vision. Visual predators in aquatic systems may be faced with poor visual conditions such as natural or human-induced turbidity. The ability of visual predators to compensate for poor visual conditions by using other senses is not well understood, although it is widely accepted that primarily visual fish, such as three-spined sticklebacks, Gasterosteus aculeatus, can detect and use olfactory cues for a range of purposes. We investigated the ability of sticklebacks to detect the presence of prey and to locate prey precisely, using olfaction, in clear and turbid (two levels) water. When provided with only a visual cue, or only an olfactory cue, sticklebacks showed a similar ability to detect prey, but a combination of these cues improved their performance. In open-arena foraging trials, a dispersed olfactory cue added to the water (masking cues from the prey) improved foraging success, contrary to our expectations, whereas activity levels and swimming speed did not change as a result of olfactory cue availability. We suggest that olfaction functions to allow visual predators to detect rather than locate prey and that olfactory cues have an appetitive effect, enhancing motivation to forage

Prey aggregation is an effective olfactory predator avoidance strategy

Predator–prey interactions have a major effect on species abundance and diversity, and aggregation is a well-known anti-predator behaviour. For immobile prey, the effectiveness of aggregation depends on two conditions: (a) the inability of the predator to consume all prey in a group and (b) detection of a single large group not being proportionally easier than that of several small groups. How prey aggregation influences predation rates when visual cues are restricted, such as in turbid water, has not been thoroughly investigated. We carried out foraging (predation) experiments using a fish predator and (dead) chironomid larvae as prey in both laboratory and field settings. In the laboratory, a reduction in visual cue availability (in turbid water) led to a delay in the location of aggregated prey compared to when visual cues were available. Aggregated prey suffered high mortality once discovered, leading to better survival of dispersed prey in the longer term. We attribute this to the inability of the dead prey to take evasive action. In the field (where prey were placed in feeding stations that allowed transmission of olfactory but not visual cues), aggregated (large groups) and semi-dispersed prey survived for longer than dispersed prey—including long termsurvival. Together, our results indicate that similar to systems where predators hunt using vision, aggregation is an effective anti-predator behaviour for prey avoiding olfactory predators

Prediction and classification for GPCR sequences based on ligand specific features

Functional identification of G-Protein Coupled Receptors (GPCRs) is one of the current focus areas of pharmaceutical research. Although thousands of GPCR sequences are known, many of them are orphan sequences (the activating ligand is unknown). Therefore, classification methods for automated characterization of orphan GPCRs are imperative. In this study, for predicting Level 1 subfamilies of GPCRs, a novel method for obtaining class specific features, based on the existence of activating ligand specific patterns, has been developed and utilized for a majority voting classification. Exploiting the fact that there is a non-promiscuous relationship between the specific binding of GPCRs into their ligands and their functional classification, our method classifies Level 1 subfamilies of GPCRs with a high predictive accuracy between 99% and 87% in a three-fold cross validation test. The method also tells us which motifs are significant for class determination which has important design implications. The presented machine learning approach, bridges the gulf between the excess amount of GPCR sequence data and their poor functional characterization

Sensing and decision-making in random search

While microscopic organisms can use gradient-based search to locate resources, this strategy can be poorly suited to the sensory signals available to macroscopic organisms. We propose a framework that models search-decision making in cases where sensory signals are infrequent, subject to large fluctuations, and contain little directional information. Our approach simultaneously models an organism's intrinsic movement behavior (e.g. Levy walk) while allowing this behavior to be adjusted based on sensory data. We find that including even a simple model for signal response can dominate other features of random search and greatly improve search performance. In particular, we show that a lack of signal is not a lack of information. Searchers that receive no signal can quickly abandon target-poor regions. Such phenomena naturally give rise to the area-restricted search behavior exhibited by many searching organisms

The role of two anatomically separate olfactory bulbs in shark food odor tracking

Most sharks have well-developed olfactory systems and depend to a large degree on odor information to locate food, home and navigate, and possibly detect predators and mates. The aim of this investigation is to determine the behavioral function of two paired bilateral olfactory bulbs in the smooth dogfish shark, Mustelus canis. The paired olfactory bulbs are a rare and unique feature among elasmobranchs and are absent in bony fishes. Given that the olfactory system of bony fishes contains lateral and medial nerve bundles with behavioral functions in feeding and social behavior respectively, we hypothesize that sharks have an elaborate functional division in which the medial bulb is processing social odors and the lateral bulb food odors. This functional division would parallel the division into an olfactory and an accessory olfactory system, also known as the vomeronasal organ or Jacobson’s organ, which evolved in tetrapods. Our study is based on the behavioral effects of selective transection of the two olfactory tracts to reveal how the brain is processing input from two anatomically distinct olfactory systems. The results show that animals with lateral tract transections showed impaired ability to track a food odor plume while those with medial transections showed no change. Attempts to identify a reliable social odor (pheromone) were not successful, preventing us from determining the deficits expected from medial tract lesions