EVALUATION AND CHARACTERIZATION OF BIOACTIVE COMPOUND OF HAMELIA PATENS PLANT WITH THE GC-MS SPECTROSCOPY

Objective: The aim of this study is to evaluate the bioactive compound present in the leaves, root, flower, and stem by gas chromatography (GC) analysis. Novel drugs are isolated from plants against human diseases.Methods: The plant parts (leaves, stem, flower, and root) were collected, washed, shade dried, and powdered, and the methanol extracts of all plant parts were prepared by Soxhlet reflux method. The methanolic extracts were analyzed for the identification of phytochemical compounds present in the Hamelia patens plant parts using GC-mass spectrometry (MS) matched by the National Institute of Standards And Technology-11 library and Willey 8 library.Results: The bioactive fraction on GC-MS analysis revealed a chromatogram showing highest peaks. Methanol extracts of H. patens leaf are found to possess a wide range of saturated and unsaturated aliphatic hydrocarbons and their isomers such as 2,3-dihydro-3,5-dihydroxy-6-methyl- 4H-pyran (1.77%), 1,3-propanediol, 2-ethyl-2-(hydroxymethyl) (3.06%), mome inositol (18.22%), pentadecanoic acid (1.66%), and squalene (11.47%). GC-MS analysis revealed chromatogram, showing that the highest peaks of H. patens stem are methyl salicylate (3.41%), 2-amino-9-(3,4- dihydroxy-5- hydroxymethyl) (9.53%), mome inositol (63.73%), and squalene (1.07%).Conclusion: Isolation of such bioactives and their use as therapeutic target is the prime motto of our research to isolate novel bioactive that can used as potential therapeutic agents

Leishmanicidal activity of alkaloids from Hamelia patens

The crude ethanolic extract from the leaves of Hamelia patens Jacq. was active in vitro against Leishmania mexicana. Partition of the extract, showed that the activity was confined to the dichloromethane extract. The four alkaloids isolated from this extract: isopteropodine (1), palmirine (2), rumberine (3) and mitrajavine (4) were evaluated in vitro for antileishmanial activity. Palmirine (2), one of the main alkaloids, showed the highest leishmanicidal activity with IC50= 56 µM, the alkaloid mitrajavine (4) and the two flavonoids isolated from the ethyl acetate extract: kaempferol-3-O-rutinoside (5), and (-) epicatechine (6) are reported for the first time in this plant