Coordination of opposing sex-specific and core muscle groups regulates male tail posture during Caenorhabditis elegans male mating behavior

Background To survive and reproduce, animals must be able to modify their motor behavior in response to changes in the environment. We studied a complex behavior of Caenorhabditis elegans, male mating behavior, which provided a model for understanding motor behaviors at the genetic, molecular as well as circuit level. C. elegans male mating behavior consists of a series of six sub-steps: response to contact, backing, turning, vulva location, spicule insertion, and sperm transfer. The male tail contains most of the sensory structures required for mating, in addition to the copulatory structures, and thus to carry out the steps of mating behavior, the male must keep his tail in contact with the hermaphrodite. However, because the hermaphrodite does not play an active role in mating and continues moving, the male must modify his tail posture to maintain contact. We provide a better understanding of the molecular and neuro-muscular pathways that regulate male tail posture during mating. Results Genetic and laser ablation analysis, in conjunction with behavioral assays were used to determine neurotransmitters, receptors, neurons and muscles required for the regulation of male tail posture. We showed that proper male tail posture is maintained by the coordinated activity of opposing muscle groups that curl the tail ventrally and dorsally. Specifically, acetylcholine regulates both ventral and dorsal curling of the male tail, partially through anthelmintic levamisole-sensitive, nicotinic receptor subunits. Male-specific muscles are required for acetylcholine-driven ventral curling of the male tail but dorsal curling requires the dorsal body wall muscles shared by males and hermaphrodites. Gamma-aminobutyric acid activity is required for both dorsal and ventral acetylcholine-induced curling of the male tail and an inhibitory gamma-aminobutyric acid receptor, UNC-49, prevents over-curling of the male tail during mating, suggesting that cross-inhibition of muscle groups helps maintain proper tail posture. Conclusion Our results demonstrated that coordination of opposing sex-specific and core muscle groups, through the activity of multiple neurotransmitters, is required for regulation of male tail posture during mating. We have provided a simple model for regulation of male tail posture that provides a foundation for studies of how genes, molecular pathways, and neural circuits contribute to sensory regulation of this motor behavior

The mating behavior and reproduction performance in a multi-sire mating system for pigs

An important aim of organic animal production is to allow natural animal behaviour. Regarding reproduction techniques, artificial insemination is permitted but natural mating is preferred. The outdoor multi-sire system, where the sows are placed in large paddocks with a group of boars, is one example of a service system, which complies well with the organic ideals of facilitating natural animal behavior. However, very little knowledge is available about such system. Seven groups of in total of 47 sows and 31 boars were observed to study the mating behavior in an outdoor multi-sire mating system and the subsequent reproduction results. The time of start of courtship, behavior and the cause of disruption if the courtship was terminated, were recorded each time a boar courted a sow. All aggressive interactions between the boars were also recorded to estimate the boar ranking order. The observations revealed numerous poor quality matings, a huge variation in the number of times sows are mated, and overworked boars. Only 35% of all copulations lasted 2 min or more and 63% of all copulations were disrupted, mainly by competitor boars. The higher social status of the boar, the more copulations did it disrupt ( p < 0.05). The outcome was an unacceptable variation in reproduction results. Only 71% of all estrus sows conceived, corresponding to a pregnancy rate of 77% of all mated sows. A large inter-group variation in reproduction performance was observed, indicating scope for improvements. In some groups all sows showed estrus and all sows conceived. Recommendations for improvement of the system are proposed

The Effects of Insecticide Treated Netting on Male-Female Interactions in Red Flour Beetles

Courtship behaviors take place before and after copulation for the purpose of stimulating the female (Evardsson & Arnqvist 2000). Females will mate with different males within minutes of the first copulation (Pai &Yan 2003), with males showing a preference for virgin females (Lewis & Iannini 1995). Red flour beetles (Tribolium castaneum) show a decrease in progeny output following exposure to insecticide treated netting (Scheff et al. in prep). To assess whether females exposed to insecticide treated netting had decreased courtship attempts, virgin females were placed with a single male to accurately determine the number of mating pair interactions. Courtship behavior and mating pair interactions were recorded to determine if the number of mating pair interactions will decrease after exposure to insecticide treated netting. Our results indicate no significant difference between the number of interactions between females exposed to control netting or insecticide treated netting. However, with females exposed to insecticide treated netting the duration of interactions increased and more interactions were initiated by males. These results suggest that the decline in progeny output may be due to reallocation of reproductive resources following exposure to insecticide treated netting and not due to decreased numbers of mating attempts

A Comparison of Two Methods of Quantifying Mating Success in Low Density Gypsy Moth (Lymantria dispar) Populations

The gypsy moth (Lymantria dispar) is a defoliating pest native to Europe and invasive to North America. The gypsy moth is subject to depressed mating success in low density populations, which may restrict spread of the forest pest. Research focusing on gypsy moth density as it relates to mating behavior has often used counts of males caught in pheromone-baited delta traps as a proxy to estimate the probability of female mating success. The purpose of this project was to determine whether pheromone trap counts provide accurate estimates of female mating success probability, by comparing data gathered from pheromone-baited delta traps to data gathered on mating success of tethered females. To determine the relationship between number of males caught in delta traps and the probability of successful mating, male catch counts in traps were compared to mating success of tethered females in a mass male release experiment. The relationship between delta trap catch of males and female mating success was quantified using a Bayesian framework, which explicitly incorporates uncertainties in the model. Vegetative cover in the study plots was reduced to a single measure through principal components analysis and included as an independent factor in the model. The data suggest that delta traps reduce the male’s ability to find a female by 67%; thus, results garnered from delta trap catch counts tend to underestimate the underlying ability of males to locate and mate with females. Thick understory vegetation further reduced the male’s ability to locate a female, and further reduced the effectiveness of delta traps. Future studies that seek to use counts of males in pheromone-baited traps as a proxy for mating success should consider using an adjustment factor to equate the two methods of quantifying reproductive behavior in the gypsy moth.https://scholarscompass.vcu.edu/uresposters/1271/thumbnail.jp

Simultaneously hermaphroditic shrimp use lipophilic cuticular hydrocarbons as contact sex pheromones

Successful mating is essentially a consequence of making the right choices at the correct time. Animals use specific strategies to gain information about a potential mate, which is then applied to decision-making processes. Amongst the many informative signals, odor cues such as sex pheromones play important ecological roles in coordinating mating behavior, enabling mate and kin recognition, qualifying mate choice, and preventing gene exchange among individuals from different populations and species. Despite overwhelming behavioral evidence, the chemical identity of most cues used in aquatic organisms remains unknown and their impact and omnipresence have not been fully recognized. In many crustaceans, including lobsters and shrimps, reproduction happens through a cascade of events ranging from initial attraction to formation of a mating pair eventually leading to mating. We examined the hypothesis that contact pheromones on the female body surface of the hermaphroditic shrimp Lysmata boggessi are of lipophilic nature, and resemble insect cuticular hydrocarbon contact cues. Via chemical analyses and behavioural assays, we show that newly molted euhermaphrodite-phase shrimp contain a bouquet of odor compounds. Of these, (Z)-9-octadecenamide is the key odor with hexadecanamide and methyl linoleate enhancing the bioactivity of the pheromone blend. Our results show that in aquatic systems lipophilic, cuticular hydrocarbon contact sex pheromones exist; this raises questions on how hydrocarbon contact signals evolved and how widespread these are in the marine environment

Comparative Aspects of Mating Behavior Patterns in Six Species of Stink Bugs (Heteroptera: Pentatomidae)

Mating sequences were analyzed for six species of stink bugs using video- tapes. The results consisted of qualitative descriptions of the precopulatory activities of the pairs and quantitative analyses of the number and direction of mating sequences, including the latency to and duration of copulatory lock. It was possible to quantitatively characterize each of the six species tested. In addition, certain infrequent behavior patterns, e.g., head butts, were observed for some species and not others. The results extend the previous information on mating activities in stink bugs, particularly for Euschistus. We interpret our findings with regard to reproductive strategies in different species of stink bugs, and consider the use of behavior as a taxonomic tool

Duplicated female receptacle organs for traumatic insemination in the tropical bed bug Cimex hemipterus: adaptive variation or malformation?

During mating, male bed bugs (Cimicidae) pierce the female abdomen to inject sperm using their needle-like genitalia. Females evolved specialized paragenital organs (the spermalege and associated structures) to receive traumatically injected ejaculates. In Leptocimex duplicatus, the spermalege is duplicated, but the evolutionary significance of this is unclear. In Cimex hemipterus and C. lectularius, in which females normally develop a single spermalege on the right side of the abdomen, similar duplication sometimes occurs. Using these aberrant morphs (D-females) of C. hemipterus, we tested the hypothesis that both of the duplicated spermaleges are functionally competent. Scars on female abdominal exoskeletons indicated frequent misdirected piercing by male genitalia. However, the piercing sites showed a highly biased distribution towards the right side of the female body. A mating experiment showed that when the normal insemination site (the right-side spermalege) was artificially covered, females remained unfertilized. This was true even when females also had a spermalege on the left side (D-females). This result was attributed to handedness in male mating behavior. Irrespective of the observed disuse of the left-side spermalege by males for insemination, histological examination failed to detect any differences between the right-side and left-side spermaleges. Moreover, an artificial insemination experiment confirmed that spermatozoa injected into the left-side spermalege show apparently normal migration behavior to the female reproductive organs, indicating an evolutionary potential for functionally-competent duplicated spermaleges. We discuss possible mechanisms for the evolutionary maintenance of D-females and propose a plausible route to the functionally-competent duplicated spermaleges observed in L. duplicatus