Efektifitas Inseminasi Buatan Pada Sapi Potong Menggunakan Semen Cair

This research aims to determine the level of effectiveness of artificial insemination in Locally bull using liquid cement based on conception rate (CR) and service per conception (S/C) in Kab. Mamuju Utara Sulawesi Barat Province. And to study the economic value of artificia insemination using liquid semen on local bull in Kab. Mamuju Utara Sulawesi Barat Province. This research used survey methode and record 109 cattle that have been inseminated from 36 farmer in Kabupaten Mamuju Utara Sulawesi Barat Province. Liquid semen that used is from Simental, Limosin and Bali breed. The data is analised by deskripted statistic. Result of this research is shown 93,3 % (94 : 109 cattle) or S/C is 1,08. The cost of using liquid semen on the implemantation AI per pregnancy is Rp. 178.200,- lower178% compared by AI using a frozen semen. Further research is needed about AI using a liquid semen from local region

Effectiveness of a novel extraction method for semen: comparison using liquid samples and dried stains

Forensic analysis of deoxyribonucleic acid (DNA) collected from sexual assault evidence is a multi-step process that requires a great amount of time and resources. A large percentage of samples are mixtures containing DNA from a major female contributor and at least one minor male contributor. The amount of male DNA present is often much less than that of the female, making it difficult to achieve a full short-tandem repeat (STR) profile for identification purposes. The current method employed by many forensic laboratories to separate sperm DNA from non-sperm DNA is the differential extraction. Although a robust and reliable method when applied to liquid samples, the procedure has failed to evolve significantly since first developed.1,2 Between the time it has been collected and tested, sexual assault evidence becomes dried and aged, contributing to the potential loss and degradation of already low amounts of DNA and increasing the likelihood of an incomplete profile.2 This study tests the effectiveness of a combination of enzymes to release DNA from sperm using a variety of substrates. Although this method extracted greater amounts of male DNA than the traditional Qiagen® extraction, further research is necessary to determine if the application of this new method can improve or eventually replace the current procedures.2018-06-16T00:00:00

Peranan Fruktosa, Rafinosa, Dan Trehalosa Pada Kriopreservasi Semen Kuda

The objective of this study was to evaluate the effectiveness of carbohydrates supplementation on the stallion semen cryopreservation. Semen was collected from 3 stallions using artificial vagina twice a week. Collected semen was evaluated macro-and microscopically. Semen showed >60% progressive motility was then divided into 3 tubes and diluted with skim milk 1:1, centrifuged at 1006 g for 10 minutes. Supernatan was removed and each pellet rediluted either with skim milk extender supplemented with 50 mM trehalose (ST); 50 mM raffinose (SR) or 100 mM fructose (SF) with the concentration of sperm were 200x106 ml-1. Extended semen packed into minitub 0.3 ml and equilibrates at 4 oC for 2 hours, freezes in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196 oC for further evaluation. After 24 hours, the semen was thawed at 37 oC for 30 second. The results of this experiment indicated that trehalose supplementation in skim milk was found to significantly improve the percentage of sperm motility (P<0.05) for stallion 1 and 3 compared to raffinose and fructose. But in all stallions, trehalose and fructose were superior compared to raffinose

Cryoprotectant-free vitrification of human spermatozoa in new artificial seminal fluid

Vitrification is a new method that has been recently introduced in Assisted Reproduction Technique programs. The aim of this study was to design a new medium similar to normal human seminal fluid (SF), formulation artificial seminal fluid (ASF), and to compare the cryoprotective potency of this medium with SF and human tubal fluid (HTF) medium. Thirty normal ejaculates were processed with the swim-up technique and sperm suspensions were divided into four aliquots: (i) fresh sample (control); (ii) vitrification in HTF medium supplemented with 5 mg/mL human serum albumin and 0.25 mol sucrose (Vit HTF); (iii) vitrification with patients' SF (Vit SF); and (iv) vitrification in ASF (Vit ASF). After warming, sperm parameters of motility, viability, and morphology were analyzed using WHO criteria. Also, sperm pellets were fixed in 2.5% glutaraldehyde and processed for scanning electron microscopy and transmission electron microscopy observations. The results showed that progressive motility (46.09 ± 10.33 vs. 36.80 ± 13.75), grade A motility (36.59 ± 11.40 vs. 16.41 ± 11.24), and normal morphology (18.74 ± 8.35 vs. 11.85 ± 5.84) and viability (68.22 ± 10.83 vs. 60.86 ± 11.72) of spermatozoa were significantly higher in Vit ASF than in Vit HTF. All parameters were better in Vit ASF than in Vit SF, but only viability was significantly different (p = 0.006). After cryopreservation, deep invagination in cytoplasm and mechanically weak point sites and folded tail were commonly observed. But, this phenomenon was more significant in Vit HTF and Vit SF than in ASF (p < 0.05). In transmission electron microscopy evaluation, acrosome damage, plasma membrane loss, chromatin vacuolation, and disruption of mitochondria arrangement and structures were observed in all vitrified groups. Adherence of several tail sections together was also seen in all cryo groups. But this was seen more in Vit HTF and Vit SF than in ASF (p < 0.05). In conclusion, vitrification of human spermatozoa with ASF can effectively preserve the quality of sperm motility in comparison with Vit HTF. © 2016 American Society of Andrology and European Academy of Andrology

Pemanfaatan Casa Dalam Observasi Motilitas Spermatozoa Semen Cair Sapi Madura Dalam Pengencer Berbeda

The purpose of this study was to measure the motility of Madura bullspermatozoa using three different diluents (tris aminomethane, CEP-2 andskim milk) duringcold storage. The research material used were 2 Madurabulls. Collecting semen method used artificial vagina, followed by freshsemen analysis and processing of liquid semen. Observation of liquid semenquality was carried out up to the 5th days of cold storage. Motilityexamination of semen liquid used Computerized Assisted Semen Analysis(CASA), namely SCA v.5.2. The parameters measured were: progressive motility,motility, velocity straight linear (VSL), velocity curve linear (VCL),velocity average pathway (VAP), straightness (STR), linearity (LIN), wobble(WOB), hyperactivity (H), Beat cross frequency (BCF), amplitude lateral head(ALH). Data was analyzed by Minitab 17. Motility and progressive motility ofspermatozoa in tris aminomethane and CEP-2 were higher than skim milk for 5days of cold storage. The VCL value of tris aminomethane was higher thanCEP-2 and skim milk. VSL scores barely show the difference among diluents.VAP values in tris aminomethaneand CEP-2 are higher than skim milk at thebeginning of storage. The LIN, STR and WOB values of the CEP-2 and skim milkyield higher values than tris aminomethane. The percentage value ofhyperactivity spermatozoa was same among diluents. The ALH value oftrisaminomethane was higher than CEP-2 and skim milk. BCF values in CEP-2was higher than skim milk and tris aminomethane. Tris aminomethane and CEP-2can support the motility of Madura bull spermatozoa than skim milkfor 5 daysof storag

The effects of extender type, freezing and thawing rates on fertility of the cryopreserved semen of the Caspian brown trout (Salmo trutta caspius)

Cryopreservation of semen from the Caspian brown trout (Salmo trutta caspius) and effects of extender type, freezing and thawing rates on fertilization ability were studied. After assessment of semen quality, one part of semen was diluted with three parts of different extender and decanted into 0.5ml straws. Freezing was carried out at two freezing levels, 1.5cm and 2cm above surface of liquid nitrogen. The semen was thawed at 5°C for 90s, 15°C for 45s or 25°C for 30s in water baths and used for fertilization. Using the extender: 0.3 M glucose+10% methanol+10% egg yolk, and 0.6 M sucrose 10% DMSO + 10% egg yolk, yielded the highest post-thaw fertilization rates, with 67.05%±8.76 and 59.78%±5.08 eyeing rates, respectively. No significant differences were found in the fertilization rates with two freezing levels (P>0.05), however eyeing and hatching rates were higher for 2cm above the surface of the liquid nitrogen than for 1.5cm. Thawing of cryopreserved semen was best using the 25°C water bath for 30s and significant differences were seen in the eyeing rates between 25°C and 5°C or 15°C (P<0.05). Significant interactions (P<0.05) were found between effects of extender type and thawing rates and extender type and freezing rates

Low-Cost QCM Sensor System for Screening Semen Samples

Artificial insemination is a well-established part of modern agricultural practice. A viable semen sample is judged by the total number of spermatozoa (sperm) in the sample and the motility of the sperm. In this paper, we report the development of a reusable measurement cell and electronics for screening semen samples based on the Quartz Crystal Microbalance (QCM) and Universal Frequency to Digital Converter (UFDC-1) to produce a low-cost sensor system. After introducing the semen sample at one end of the measurement cell, sperm swim down a channel before causing a frequency change on the QCM. Data is presented that shows the different frequency changes using a commercial frequency counter caused by porcine semen samples, one two days old and one twenty one days old. Similar data is presented for a motile semen sample measurement using the low-cost UFDC-1