A decade of studies at Loch Fleet, Galloway (Scotland): A catchment liming project and restoration of a brown trout fishery

Loch Fleet is a small upland lake in the hills of Galloway in southwest Scotland. In the 1970s the waters of the loch became more acidic and a brown trout fishery failed. This account summarises an experimental project, the "Loch Fleet Project" initiated in 1984, designed to reverse acidification of the loch by liming parts of the catchment. Liming about 40% of the catchment in 1986 and 1987 raised the pH and calcium levels, and reduced toxic aluminium concentrations. The improved conditions had been maintained up to 1994, but water in the loch, and its principal inflow stream, is now falling close to the desired threshold of quality. After liming, restocking with local strains of trout in 1987 was successful, and a self-recruiting population became established within 2 years. Recruitment is highly variable, however, and was attributed to severe spring conditions and the limited spawning capacity of the nursery stream, rather than to water quality

Land Improvements Under Land Tenure Insecurity: The Case of Liming in Finland

This article solves and characterizes optimal decision rules to invest in irreversible land improvements conditional on land tenure insecurity. Economic model is a normative dynamic programming model with known parameters for the one period returns and transition equations. The optimal decision rules for liming are solved numerically, conditional on alternative scenarios on the likelihood that the lease contract and, thus, farmer access to land is either renewed or expired. The model parameters represent Finnish soil quality and production conditions. The results suggest that irreversible liming decreases quickly and the yields decline gradually, when the farmer is confronted with land tenure insecurity caused by uncertain renewal of the lease contract. The results are confirmed via empirical results.Land tenure, Liming, Land Economics/Use,

Liming Ohio's Acid soils

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Liming Ohio Soils

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Effect of liming on yield and quality of peppermint and Sachalin mint in fine sand soil of Northern Finland

Soil acidity commonly limits plant production in the fine sand soil of Northern Finland, which often has a low pH (5.5-6.5) and contains low levels of Ca and Mg. The effect of five liming (10% Mg and 19% Ca) levels, 0, 4, 8, 12, and 16 tons ha -1 , on the herb and essential oil yield and menthol and menthone content of two mint species (peppermint, Mentha x piperita, a variety of Black Mitcham and Sachalin mint, Mentha arvensis var. sacchalinensis) cultivated in fine sand soil in Northern Finland (6440’N and 2505’E) was studied during 1998-2000. Liming clearly increased the pH levels and the Ca and Mg content of the soil. The dry matter content, essential oil quantity, and the menthol or menthone content in mints were not affected by liming. In comparison with no liming however, liming at a rate of 4 t ha -1 doubled the herb yield. The highest yield was achieved in Sachalin mint by liming at 4 or 8 t ha -1 in the second and third year (soil pH 6-6.5) (Ca 725-871 mg l -1 and Mg 122- 219 mg l -1 ), and in peppermint by liming at 4, 8 or 16 t ha -1 (soil pH 6-6.6) (Ca 725-1272 mg l -1 and Mg 122-245 mg l -1 ). Therefore, we conclude that a higher peppermint and Sachalin mint yield is achieved by increasing soil pH to values above 6.0 in the fine sand soil of Northern Finland

Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents

Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent. This study was therefore carried out to identify simple but effective methods for isolation and identification of bacterial pathogens from the effluent and skins during leather processing. Identification methods based on both phenotypic and genotypic characteristics were investigated. Bacillus cereus and Pseudomonas aeruginosa were used as indicator bacteria to evaluate the isolation and identification methods. Decontaminated calfskins were inoculated with a pure culture of the above mentioned bacterial species followed by a pre-tanning and chromium tanning processes. Effluent samples were collected and skins were swabbed at the end of each processing stage. Bacterial identification was carried out based on the phenotypic characteristics; such as colony appearance on selective solid media, cell morphology following a standard Gram-staining and spore staining techniques, and biochemical reactions, e.g., the ability of a bacterial species to ferment particular sugars and ability to produce certain enzymes. Additionally, an identification system based on bacterial phenotypic characteristics, known as Biolog® system was applied. A pulsed-filed gel electrophoresis (PFGE) method for bacterial DNA fingerprinting was also evaluated and used for the identification of the inoculated bacteria. The methods described in the study were found to be effective for the identification of pathogenic bacteria from skins and effluent

Can biochar ameliorate phosphorus deficiency and aluminium phytotoxicity in acid soils? : a thesis presented in partial fulfilment of the requirements for the degree of Doctor of Philosophy of Applied Science in Soil Science at Massey University, Palmerston North, New Zealand

The use of biochar as soil amendment to enhance soil functionality is being increasingly investigated, with particular attention given to its effects on the sustainable increase of crop production and carbon (C) sequestration. To date, however, limited research has attempted to unravel the effect of biochar on either the chemical and/or biological mobilization of the residual fraction of phosphorus (P) in soil. This fraction tends to accumulate as a result of long–term P fertilization in soils rich in aluminium (Al) and iron (Fe) oxy–hydroxides and short–range ordered aluminosilicates (i.e. allophane). There is also scant information on (i) how the speciation of soluble Al changes when biochar is applied to acid soils, and (ii) whether this application alleviates Al toxicity on plant roots. My objective in this study is therefore to determine the effect of different biochars with contrasting fertilizer and liming values on the chemistry, biology and nutrient fertility of acid mineral soils. Before studying the effect of different biochars on soil properties, several methodologies for measuring the liming properties and available nitrogen (N) in biochar were evaluated and modified where needed. For this, 19 biochars produced by pyrolysing a wide range of feedstocks under various production temperatures were used. Different pH–buffering capacity (pH–BC) methodologies – originally developed for soils (single vs multiple acid additions, short vs long equilibration times) – were tested, along with the methodology used to measure the liming equivalence. The methodologies were then validated by incubating over 10 d two acid soils (an Haplic Cambisol and an Andic Umbrisol) to which separated amendments of the 19 biochars were made at the rates estimated using both methodologies to target a final pH of 6.5 The results indicated that the relationship established between the pH–BC of the 19 biochars under study after 30–min equilibration (pH–BC30min) with a single addition of acid and that obtained after a 5–d equilibration (pH–BC5d) (predicted pH–BC5d = 2.2 × pH–BC30min + 20.4) allowed an adequate estimate of the liming potential of biochars. Similar results were found with the liming equivalent, and both methods were considered suitable to make the recommendation of the application rate. Acid hydrolysis using 6 M HCl has been proved adequate to determine available N in biochar. For this, hydrolysates of biochar are oxidized using potassium peroxodisulfate with a dilution factor of 600 so that chloride interferences are overcome and nitrate–N is then measured. This methodology, originally developed using biochars rich in N, proved not suitable for biochars with low N concentrations. Results obtained in this study have shown that a smaller dilution factor (242) is sufficiently adequate to overcome the chloride interferences while avoiding over–diluting the sample. In this study, we hypothesized that biochar can increase P availability to plant by stimulating the growth of arbuscular mycorrhizal fungi (AMF) hyphae. Therefore, methodologies to (i) estimate the length of fungal hyphae in soil and (ii) evaluate the transfer of P by AMF hyphae needed to be tested and modified where necessary. In this part of the study, three different biochars and two soil types were used. Two biochars were produced from chipped pine (Pinus radiata D. Don) branches at 450oC and 550°C (referred to as BP450 and BP550, respectively); and a third one from chipped weeping willow (Salix matsudana L.) branches at 550°C (referred to as BW550). The soils were two sil–andic Andosols of contrasting P status (Olsen P of 4.3 vs 33.3 mg kg–1, referred to as LP and HP soil, respectively). The traditional visual gridline intersection (VGI) method commonly used to measure the length of AMF hyphae distribution in soil was modified by (i) using a digital photomicrography technique (referred to as “digital gridline intersection” (DGI) method), and (ii) processing the images using ImageJ software (referred to as the “photomicrography–ImageJ processing” (PIP) method). These methods were first tested with known lengths of possum fur and then applied to measuring the hyphal length in the LP and HP soils after a 32 wk experiment growing Lotus pedunculatus cv barsille. The study confirmed that the use of digital photomicrography in conjunction with either the grid–line intersection principle or image processing (with ImageJ software) is a suitable method for the measurement of AMF hyphal lengths in soils. In addition, the traditional root study container that divides the plant growth medium into two sections – (i) a root zone to which both root and AMF hyphae have access and (ii) an hyphal zone to which only AMF hyphae have access – by a layer of nylon mesh was further modified by including a 3–mm thickness of tephra under the nylon mesh between two sections. This layer of tephra was found to be adequate to halt P diffusion from the HP soil to the LP soil for a plant growth period of 32 wk. Under such circumstances, the increase in P uptake by plant growth in a combination of a root zone of LP soil and a hyphal zone of HP soil compared with that in which both root and hyphal zones were filled with LP soil was only ascribed to the transfer of P from HP soil to LP soil by AMF hyphae. This novel root study container allows the biochar to be added to either the root zone or the hyphal zone and separates the effect of biochar on AMF hyphae development and P uptake from that on P content and availability (i.e., biochar rich in P; changes in soil pH). This device can contribute to discern whether biochar can influence AMF development and enhance P bioavailability. In order to investigate the feasibility of adding biochar to soils with high residual P so that this can become bioavailable, Lotus pedunculatus cv barsille was grown in LP and HP soils separately amended with BP450, BP550 and BW550 biochars at an application rate of 10 t ha–1 using the novel root study container for 32 wk without any further P and N fertilization. We found that (i) none of the tested biochars conferred any specific advantage to the HP soil; (ii) the addition of BW550 biochar to the LP soil increased plant growth by 59% and P uptake by 73%, while the pine–based biochar (e.g., BP450 and BP550) provided no extra nutrient uptake and no plant growth increase. This was ascribed to supplemental nutrients (especially P) from the BW550 biochar along with its liming effect and associated increase in P availability; (iii) biochar produced from BP450 biochar caused a 70% P uptake increase (and 40% plant growth increase) by stimulating AMF growth and accessing a high–P area (HP soil) to which the plant root had no access. More research is needed to discern the underlying mechanism. The liming effects of BW550 and BP550 biochars were further compared with those of lime chemicals (e.g., Ca(OH)2 and NaOH) in a short–term (10–d) incubation using two soils with contrasting pH–BC (an Haplic Cambisol and an Andic Umbrisol) to which these amendments were added. The two soils were first amended with BW550, BP550, Ca(OH)2 or NaOH at specific rates so that pH values of 5.4, 5.6, 5.8 and 6.4 were targeted and incubated at room temperature (25 oC) for 10 d. At the end of the incubation, a radical elongation bioassay using alfalfa (Medicago sativa L.) was carried out. Thereafter, soils were characterized with special attention to the Al chemistry, i.e. aqueous reactive Al fractionation and inorganic monomeric Al speciation. The final objective was to reveal the mechanisms through which these biochars alleviate Al toxicity on roots. Results showed that, for a specific soil, a smaller amount of BW550 biochar was required to increase the same unit of pH and reduce a similar amount of exchangeable Al compared with the amount required of BP550 biochar. The addition of BW550 biochar (at application rates < 9.1 %) and Ca(OH)2 stimulated alfalfa (Medicago sativa L.) seedling growth, whereas that of BP550 (at application rates > 2.4 %) and NaOH caused inhibition. The distinct responses of the root growth to the presence of Ca(OH)2 and BW550 biochar and to that of NaOH and BP550 biochar were explained by (i) a decrease in both inorganic monomeric Al (mainly in AlF2+ and Al3+) and colloidal Al, and (ii) an increase in aqueous Ca2+, in the former, as expected. In the latter there was (i) an increase in aqueous colloidal Al and Na+, and (ii) a decrease in soluble Ca2+. Thus, BW550 biochar was shown to be a more effective liming agent than was BP550 biochar. The information obtained in this thesis supports the use of biochar to manage high P affinity Andosols and acid soils, which are abundant in New Zealand. The technology of producing biochar from willow woodchips or feedstock alike with resultant solid products of high nutrient status and liming potential may contribute to the recycle of nutrients while increasing soil pH. Pine woodchips produced at relatively low temperature (e.g., 450oC) have been shown to enhance AMF abundance and functionality. Thus, biochar with specific environmental and agricultural purposes should be tailored accordingly. The root study container with a layer of “P diffusion break” and the measurement of AMF hyphal length using the photomicrography in conjunction with image software analysis (e.g., ImageJ) will advance studies of the responses of AMF to soil additives (e.g., biochar or green waste) and their associated enhancement of soil functions