Inhibition of Bifidobacterium SP Isolated From Infants Feces Towards Adhesion of Salmonella Typhi on Balb/c Mice Enterocyte

Diarrhea, up to the recent year remains a cause of high morbidity and mortalityworldwide, especially in developing countries including Indonesia. Research concerning ofmanagement, prevention, and medication of the disease have been continually improved. Theaim of this research is searching Bifidobacterium sp isolated from infants feces. ThisBifidobacterium was then applied as an anti-adhesion of Salmonella typhi in the hope to gain acure of diarrhea. This research employed two research designs, namely descriptive explorationand true experimental. Exploration was applied in order to obtain isolation and characterizationof Bifidobacterium isolated from infants feces. Adherence ability of this Bifidobacterium sptowards Salmonella typhi adhesion on mice entherocyte was then carried out by applyingRandomized Posttest-Only Control Group Design. In this research, average Bifidobacterium spadhesion index of 1950 on entherocyte was obtained. In simple word, there are 19.5Bifidobacteria adhere to any single entherocyte cell. This adhesion index value is highercompare to Salmonella typhi adhesion of 1504. Conclusions that can be drawn from this researchare the finding of Bifidobacterium sp isolated from infants feces. This Bifidobacterium sp has anability to inhibit adhesion of Salmonella typhi on BALB/c mice enterocyte. Future work that canbe carried out are further researches concerning whether these bacteria have an ability to inhibitadherence of other pathogen bacteria. More over, searching of cell wall adhesin ofBifidobacterium sp that can be used as a replacement of life probiotic bacteria is also a greatinterest of research to be carried out

STUDI POTENSI TOLERANSI ISOLAT FUNGI LIMBAH COMBERAN \ud TERHADAP DETERGEN, SABUN MANDI DAN SABUN COLEK KAWASAN \ud PADAT HUNI DI KOTA MALANG

Detergent and Soap is cleanser of sintetic having negative impact to health either through is direct (physical contact) and also indirectly (residing in nature or environment in the form of waste). This research aim to to know chosen fungi isolate which most have lenient potency to detergent, toilet soap and soap of colek. This process is started with insulation of fungi of waste nature, namely waste of comberan by using selective media CZAPEK.DOX.AGAR fungi. found Isolate [is] later;then given letter code and number, namely B1, B2, B3,.....etc, where (B=Fungi, \ud 1,2,3...=jenis to). [Gift/ giving] of code pursuant to difference of characteristic of makroskopis and [is] microscopic the than fungi isolate. From 21 type of isolate found by isolate of rank pursuant to amount of colony many, chosen so that 7 isolate which later; then referred with \ud chosen isolate. ideally functioning soap and detergent as cleaner and also antiseptic and disinfectant to microorganisme specially fungi, but result of insulation indicate that there are fungi which not die, so that assumed by the fungi isolate lenient and can elaborate third the materials in nature. Research type the used is descriptive with approach of laboratory, where this \ud research aim to for description concerning lenient potency of chosen fungi isolate to detergent, toilet soap and soap of colek, later; then to know the tolerance potency test by using detergent, toilet soap and soap clean concentration 25%, 50%, 75% and 95%. Smaller assess zona diameter \ud pursue {progressively come near zero ( 0)}, hence is ever greater of its tolerance potency Result of research indicate that: (1) Pursuant to range assess zona diameter pursue smallestly, hence most isolate have potency to be lenient to detergent is B8 isolate, isolate having potency \ud most lenient to toilet soap is B4 isolate, while most isolate have lenient potency to soap of colek is B19 isolate. ( 2) Storey;Level resilience of isolate fungi, to detergent: B8, B21, B2, B4, B16, B19 and of B13, for the toilet soap of: B4, B19, B16, B13, B21, B2, and B8, while soap of colek:\ud B19, B13, B8, B2, B21, B4, and B16. found of lenient fungi isolate to detergentt, toilet soap and soap of colek, can give information in area of microbiological science in the effort yielding product of inokulum microbe capable to pregnant waste degredate third disinfectant type and \ud antiseptic

Complete Genome Sequences of a Clinical Isolate and an Environmental Isolate of Vibrio parahaemolyticus.

Vibrio parahaemolyticus is the leading cause of seafood-borne infections in the United States. We report complete genome sequences for two V. parahaemolyticus strains isolated in 2007, CDC_K4557 and FDA_R31 of clinical and oyster origin, respectively. These two sequences might assist in the investigation of differential virulence of this organism

Film extrusion of sunflower protein isolate

Film extrusion of sunflower protein isolate (SFPI) was studied. The influence of die temperature (85 to 160°C), water and glycerol contents were investigated through appearance, mechanical and thermo-mechanical properties and swelling behavior in water of films. It was demonstrated that highest temperature, well above SFPI denaturation temperature in the compound, highest glycerol content (70 parts for 100 parts of SFPI) and medium water content (20 parts for 100 parts of SFPI) gave the most regular and smoothest film (as seen on SEM micrographs). Its ultimate tensile strength, Young’s modulus and strain at break were respectively: 3.2 MPa, 17.7 MPa and 73%. Soaked in water, its swelling was about 186% w/w but the film was quiet insoluble. Effect of temperature and plasticizer content were discussed in relation to the kinetic of SFPI denaturation. These first results are very promising for the development of biodegradable protein-based films

Comparative genomic analyses reveal a lack of a substantial signature of host adaptation in Rhodococcus equi (‘Prescottella equi’)

Rhodococcus equi (‘Prescottella equi’) is a pathogenic actinomycete primarily infecting horses but has emerged as an opportunistic human pathogen. We have sequenced the genome of the type strain of this species, R. equi strain C7T, and compared the genome with that of another foal isolate 103S and of a human isolate ATCC 33707. The R. equi strains are closely related to each other and yet distantly related to other rhodococci and Nocardia brasiliensis. The comparison of gene contents among R. equi strains revealed minor differences that could be associated with host adaptation from foals to humans, including the presence of a paa operon in the human isolate, which is potentially involved in pathogenesis

Investigation of Functional-technological Properties of Soya Protein

There was offered and grounded the use of functional technological properties of the soya protein isolate in the technology of oil pasts. It will allows to increase the balance of the oil past composition additionally and will favor the decrease of extracting moisture during the storage term.There was studied the dynamics of a gradient of the limit stress of soya protein: hydrated soya protein, hydrated soya protein with the temperature processing, hydrated soya protein with the preliminary keeping during 24 h, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 5 min, hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 10 min; hydrated soya protein with the preliminary keeping during 24 h and temperature processing during 15 min.It was established, that the hydrated isolate of soya protein is a plastic system, has enough strength.The limit stress parameter at the variable velocity of deformation of model samples determines optimal technological parameters of preparing the soya isolate: hydromodule – 1: 8, temperature processing – (82±2) °С, process duration 10 min with preliminary keeping during 24 hours.As a result of the studies, there were demonstrated technological parameters of preparing the soya protein isolate for obtaining the oil past by the direct mixing with the oil base

Draft genome sequence of the blaOXA-436- and blaNDM-1-harboring Shewanella putrefaciens SA70 isolate

ABSTRACT We sequenced a carbapenem-resistant Shewanella putrefaciens isolate cultured from the sink handle of a Pakistan hospital room. Assembly annotation indicates that the isolate has a chromosomal bla OXA-436 carbapenemase and a plasmid-borne bla NDM-1 gene. To our knowledge, this is the first report of a Shewanella species harboring bla NDM . </jats:p

Isolation of Local Lipolytic Isolate from Domestic Compost

Screening of lipolytic bacteria from domestic compost resulting an isolate namely AL17. Morphological analysis shows that the isolates were rod shape and belong to negative gram bacteria. The 16S rRNAs genes of the bacteria have been sequenced, and phylogenetic analysis showed that the isolates were close to genus Pseudoxanthomonas. The enzyme production was synchronized with bacterial growth and reached a maximum level during the late-stationary phase. The optimum pH and temperature of enzyme activity were at pH 9.0 and 60°C.The isolate also showed alcohol tolerance in medium containing 3% and 5% methanol. The ability of bacterial cells to tolerate methanol is an important cell characteristic that determines their use as a biocatalyst in transesterification and other industrial process

Madex Plus and Madex I12 overcome Virus Resistance of Codling Moth

Over the past three years, codling moth populations with resistance to the Mexican isolate of Cydia pomonella Granulovirus (CpGV) have been found in Germany, France, Italy, Switzerland and the Netherlands. Andermatt Biocontrol has tested two new virus isolates (Madex Plus and Madex I12) which can overcome CpGV-resistance, and compared them in the field. Both isolates proved effective against Mexican isolate resistant codling moth populations, in several locations. In Switzerland, Madex Plus has been already approved for use

Efficacy of Pseudomonas chlororaphis subsp. aureofaciens SH2 and Pseudomonas fluorescens RH43 isolates against root-knot nematodes (Meloidogyne spp.) in kiwifruit

The Root-knot nematodes, Meloidogyne spp., are parasites of many crops and orchards, including kiwifruit trees. The Islamic Republic of Iran is among the leading kiwifruit producers in the world and M. incognita has been found as the dominant species responsible for severe loss of this crop. In order to evaluate the eff ectiveness of antagonistic bacteria on larval mortality, number of galls per plant and egg masses of nematode reduction, fifty local bacterial strains were isolated from root surrounding soils of kiwifruit plants in the northern production areas in Iran. Bacterial antagonists were characterized by morphological, physiological, biochemical and molecular methods. Two representative strains, showing the best nematicidal activity, were identif ed as Pseudomonas chlororaphis subsp. aureofaciens (isolate Sh2) and Pseudomonas fluorescens (isolate Rh43). They increased the percentage of larval mortality to 56:38% and 54:28% respectively in assays in vitro and showed excellent performance also in vivo with consistent reduction of number of galls (67:31% and 55:63%, respectively) and egg mass (86:46% and 84:29%, respectively) in plants. This study indicates that Pseudomonas chlororaphis subsp. aureofaciens isolate Sh2 and Pseudomonas fluorescens isolate Rh43 are good potential biocontrol agents for containing root-knot nematodes in kiwifruit trees.peer-reviewe