48,012 research outputs found

    Planning for Basic Needs: Primary Health Care and the Community

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    Fears and the Female Circumstance: Women in 1970s Horror Films

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    [Review of] Rodney Frey. The World of the Crow Indians: As Driftwood Lodges

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    Ethnographic studies have long been plagued by questions of credibility. Can the ethnographer believe his or her sources? And, in turn, can readers believe the ethnographer? Ronald Frey knows full well that such issues of believability plague anyone attempting to understand a culture\u27s otherness from the outside. He is determined to explain general historical, religious, and cultural aspects of the world of the Crow Indians from as close to the inside as he possibly can tell them

    Genome composition of 'Elatior'-begonias hybrids analyzed by genomic in situ hybridisation

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    Interspecific hybridization of various tuberous Begonia species hybrids with Begonia socotrana results in so-called 'Elatior'-begonias hybrids (B. x hiemalis Fotsch). In our study, genomic in situ hybridization (GISH) has been employed to assess the genome composition in eleven 'Elatior'-begonias hybrids and their ancestor genotypes. Genomic DNA of tuberous Begonia was sonicated to 1-10-kb fragments, labelled by nick translation with digoxigenin-11-dUTP and used as a probe whereas B. socotrana DNA was autoclaved to 100 bp fragments and used as block. The genome of tuberous Begonia was clearly pronounced in 'Elatior'-begonias when the probe concentration was similar to 3.75 ng/mu l (150 ng/slide), with 30 times the excess of B. socotrana blocking DNA and stringency of post hybridization washings at 73% (0.1x SSC at 42A degrees C). In 'Elatior'-begonias hybrids GISH distinguished two groups comprising short (0.6-1.03 mu m in length) and relatively longer chromosomes (1.87-3.88 mu m) which represent B. socotrana and tuberous Begonia genomes, respectively. The number of chromosomes derived from tuberous Begonia ranged from 14 to 56 and for B. socotrana from 7 to 28 which suggest the presence of different ploidy levels in analyzed 'Elatior'-begonia hybrids. Intergenomic recombination has not been detected through GISH in hybrids analyzed. Genomic in situ hybridization turned out to be useful to identify the genome constitution of 'Elatior'-begonia hybrids and thus gain an insight into the origins of these cultivars. This knowledge on the ploidy level and genome composition is essential for further progress in breeding Begonias

    Interspecifikus kromoszóma átépülések indukálása és felhasználásuk a kromoszómák fizikai térképezésére a búzában = Induction of interspecific chromosome rearrangements and their use for the physical mapping of chromosomes in wheat

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    A pályázat célja idegen fajú kromoszómákat, szegmentumokat hordozó búza genotípusok előállítása volt, majd azok azonosítása fluoreszcens in situ hibridizációval (FISH) és molekuláris markerekkel. A pályázati munkában az árpából (Hordeum vulgare), egy kecskebúza (Aegilops buncialis) és egy tarackbúzafajból (Agropyron glael) történő génátvitelre koncentráltunk. Őszi búza/őszi árpa (Mv9 kr1/Igri) 2H, 3H, 4H és 1HS izokromoszómás diszómás addíciós vonalakat állítottunk elő és azonosítottunk FISH-sel illetve molekuláris markerekkel. A 4H(4D) búza(árpa) szubsztitúciós vonalból PhI szuppresszor gént hordozó búzavonallal végzett megporzással búza/árpa transzlokációkat hoztunk létre és mutattunk ki GISH-sel. Az Asakaze komugi × Manasz (búza/őszi árpa) hibrid szövettenyészetben való elszaporítása után az utódokban 3H, 5H deléciós és búza/árpa transzlokációs kromoszómákat mutattunk ki GISH-sel és térképeztünk fizikailag is molekuláris markerekkel. Besugárzással búza × Aegilops biuncialis amfiploidokban intergenomikus kromoszóma átrendeződéseket hoztunk létre és mutattunk ki multikolor GISH-sel, megkülönböztetve a búza és az Ae. biuncialis U és M genomját. A búza × Agropyron glael hibridből szövettenyészetben való elszaporítás után számos utódot állítottunk elő. Az Agropyron kromoszómák kimutatására a GISH módszer adaptálását egy búza-A. elongatum részleges amphidiploidon végeztük, amelyben intergenomikus átrendeződéseket is detektáltunk | The main objective of the present proposal was to produce wheat genotypes with alien chromosomes (segments) and to identify them with fluorescence in situ hybridization (FISH) and molecular markers. Alien gene transfer was carried out from barley (Hordeum vulgare), from a goatgrass (Aegilops biuncialis) and from an Agropyron species (A. glael). 2H, 3H, 4H and 1HS wheat/barley (Mv9 kr1/Igri) disomic addition lines were produced and identified with FISH and using molecular markers. Wheat/barley translocation lines were produced from the 4H(4D) substitution line by pollination with a wheat line carrying the PhI suppressor gene, and they were detected by GISH. 3H, 5H deletion and wheat/barley translocation chromosomes were detected by GISH in the progeny of Asakaze komugi × Manas (winter barley) hybrids after multiplication in tissue culture. The chromosomes were physically mapped using GISH and molecular markers. Intergenomic rearrangements were induced from wheat × Aegilops biuncialis amphiploids using irradiation. The translocations were demonstrated using multicolour GISH, which differentiated the wheat genome and the U and M genomes of Ae. biuncialis. Several progenies were produced from the wheat × Agropyron glael hybrids after multiplication in tissue culture. A modification of the GISH technique, allowing Agropyron chromosomes to be detected, was carried out on a wheat×Agropyron partial amphiploid, in which intergenomic translocations were observed

    The Effect of Wet Pressing on Filler Stratification

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    Research was conducted to determine the effect of press loading upon filler stratification with the intention of observing the development of a stratified sheet. Press conditions were held constant with the exception of pressure applied to the sheet. The sheet was then split and examined for filler content. The paper tested was highly refined, very moist, and of a heavy basis weight. Results indicated a gradual trend of increased stratification with increased press loading. Filler moved toward the direction that water was leaving the sheet

    Me \u27n Susie Manuscript

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    Dr. Shirley Gish\u27s original manuscript for her play, Me \u27n Susie. The play is in two acts and is performed by one performer. Dr. Gish based this play off of her interviews with Dr. Louise Caudill, Susie Halbleib, and other members of the community in Morehead, Kentucky
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