Comparative Analysis of the Major Polypeptides from Liver Gap Junctions and Lens Fiber Junctions

Gap junctions from rat liver and fiber junctions from bovine lens have similar septilaminar profiles when examined by thin-section electron microscopy and differ only slightly with respect to the packing of intramembrane particles in freeze-fracture images. These similarities have often led to lens fiber junctions being referred to as gap junctions. Junctions from both sources were isolated as enriched subcellular fractions and their major polypeptide components compared biochemically and immunochemically. The major liver gap junction polypeptide has an apparent molecular weight of 27,000, while a 25,000-dalton polypeptide is the major component of lens fiber junctions. The two polypeptides are not homologous when compared by partial peptide mapping in SDS. In addition, there is not detectable antigenic similarity between the two polypeptides by immunochemical criteria using antibodies to the 25,000-dalton lens fiber junction polypeptide. Thus, in spite of the ultrastructural similarities, the gap junction and the lens fiber junction are comprised of distinctly different polypeptides, suggesting that the lens fiber junction contains a unique gene product and potentially different physiological properties

Enhanced Andreev reflection in gapped graphene

We theoretically demonstrate unusual features of superconducting proximity effect in gapped graphene which presents a pseudospin symmetry-broken ferromagnet with a net pseudomagnetization. We find that the presence of a band gap makes the Andreev conductance of graphene superconductor/pseudoferromagnet (S/PF) junction to behave similar to that of a graphene ferromagnet-superconductor junction. The energy gap $\Delta_N$ enhance the pseudospin inverted Andreev conductance of S/PF junction to reach a limiting maximum value for $\Delta_N\gg \mu$, which depending on the bias voltage can be larger than the value for the corresponding junction with no energy gap. We further demonstrate a damped-oscillatory behavior for the local density of states of the PF region of S/PF junction and a long-range crossed Andreev reflection process in PF/S/PF structure with antiparallel alignment of pseudomagnetizations of PFs, which confirm that, in this respect, the gapped normal graphene behaves like a ferromagnetic graphene.Comment: 7.2 pages, 5 figures, accepted for publication in Phys. Rev.

The Mr 28,000 gap junction proteins from rat heart and liver are different but related

The sequence of the amino-terminal 32 residues of the rat heart Mr 28,000 gap junction protein presented here allows, for the first time, a sequence comparison of gap junctional proteins from different tissues (heart and liver). Comparison of the rat heart gap junction protein sequence and that available from rat liver reveals 43% sequence identity and conservative changes at an additional 25% of the positions. Both proteins exhibit a hydrophobic domain which could represent a transmembrane span of the junction. This result unequivocally demonstrates the existence of at least two forms of the gap junction protein. As yet, no homology is evident between the gap junctional proteins of either heart or liver and main intrinsic protein from rat eye lens

Anomalous temperature dependence of the supercurrent through a chaotic Josephson junction

We calculate the supercurrent through a Josephson junction consisting of a phase-coherent metal particle (quantum dot), weakly coupled to two superconductors. The classical motion in the quantum dot is assumed to be chaotic on time scales greater than the ergodic time $\tau_{erg}$, which itself is much smaller than the mean dwell time $\tau_{dwell}$. The excitation spectrum of the Josephson junction has a gap $E_{gap}$, which can be less than the gap $\Delta$ in the bulk superconductors. The average supercurrent is computed in the ergodic regime $\tau_{erg} \ll \hbar/\Delta$, using random-matrix theory, and in the non-ergodic regime $\tau_{erg} \gg \hbar/\Delta$, using a semiclassical relation between the supercurrent and dwell-time distribution. In contrast to conventional Josephson junctions, raising the temperature above the excitation gap does not necessarily lead to an exponential suppression of the supercurrent. Instead, we find a temperature regime between $E_{gap}$ and $\Delta$ where the supercurrent decreases logarithmically with temperature. This anomalously weak temperature dependence is caused by long-range correlations in the excitation spectrum, which extend over an energy range $\hbar/\tau_{erg}$ greater than $E_{gap} \simeq \hbar/\tau_{dwell}$. A similar logarithmic temperature dependence of the supercurrent was discovered by Aslamazov, Larkin, and Ovchinnikov, in a Josephson junction consisting of a disordered metal between two tunnel barriers.Comment: 14 pages with 2 figures; the revision corrects the published version in Eqs. 8, 15, and 21d (with thanks to Marlies Goorden

Major Loss of the 28-kD Protein of Gap Junction in Proliferating Hepatocytes

There is a reduction in the 28-kD gap junction protein detectable by immunofluorescence in livers of partially hepatectomized rats and in cultured hepatocytes stimulated to proliferate. By the coordinate use of antibodies directed to the hepatic junction protein (HJP28) and the use of a monoclonal antibody that recognizes bromodeoxyuridine (BrdU) incorporated into DNA, we have been able to study the relationship between detectable gap junction protein and cell division. Hepatocytes that label with BrdU in the regenerating liver and in cell culture show a significant reduction of HJP28. Cells that do not synthesize DNA, on the other hand, show normal levels and distribution of immunoreactive gap junction protein. We postulate that the quantitative changes in gap junction expression might play an important role in the control of proliferation in the liver

Novel Josephson effects between multi-gap and single-gap superconductors

Multi-gap superconductors can exhibit qualitatively new phenomena due to existence of multiple order parameters. Repulsive electronic interactions may give rise to a phase difference of $\pi$ between the phases of the order parameters. Collective modes due to the oscillation of the relative phases of these order parameters are also possible. Here we show that both these phenomena are observable in Josephson junctions between a single-gap and a multi-gap superconductor. In particular, a non-monotonic temperature dependence of the Josephson current through the junction reveals the existence of the $\pi$ phase differences in the multi-gap superconductor. This mechanism may be relevant for understanding several experiments on the Josephson junctions with unconventional superconductors. We also discuss how the presence of the collective mode resonantly enhances the DC Josephson current when the voltage across the junction matches the mode frequency. We suggest that our results may apply to MgB$_2$, 2H-NbSe$_2$, spin ladder and bilayer cuprates.Comment: 4 pages, 2 figure

Critical gap analysis of merging sections at Kuala Lumpur middle ring road

At merging sections, drivers normally slow down and sometimes need to stop while seeking a suitable gap before merging with the mainstream. Thus, there will always be several observed rejected gaps and an accepted gap which can be used to determine the smallest average gap, so-called critical gap. This study was carried out to determine critical gap values at selected merging sections at the Jalan Tun Razak and the DUKE Expressway uses the Maximum Likelihood method. Data were collected by using videotaping method and the gap acceptance data were extracted for analysis. A gap acceptance event at highway merging sections in this study was redefined due to unavailability of stopping vehicles at the ramp junction. Therefore, the gap data were estimated starting from a ramp’s vehicle passing the end of gore marking to where it merges with the mainstream. The analysis of the critical gap takes into consideration accepted gaps greater than 5 seconds to avoid forced entry due to lead impedance of successive vehicles on mainstream. The critical gap values obtained in this study, according to vehicle classification were ranged between 4.5 to 5.0 seconds, which are slightly smaller if compared to critical gap values for particularly left turn from minor movement at priority junction of the Arahan Teknik (Jalan) 11/87 and the United States Highway Capacity Manual 2000. The findings shall help to study driving behavior of local drivers, especially at priority control facilities such as merging sections

Coherent Operation of a Gap-tunable Flux Qubit

We replace the Josephson junction defining a three-junction flux qubit's properties with a tunable direct current superconducting quantum interference devices (DC-SQUID) in order to tune the qubit gap during the experiment. We observe different gaps as a function of the external magnetic pre-biasing field and the local magnetic field through the DC-SQUID controlled by high-bandwidth on chip control lines. The persistent current and gap behavior correspond to numerical simulation results. We set the sensitivity of the gap on the control lines during the sample design stage. With a tuning range of several GHz on a qubit dynamics timescale, we observe coherent system dynamics at the degeneracy point.Comment: 3 pages, 4 figure

The connexin43 mimetic peptide Gap19 inhibits hemichannels without altering gap junctional communication in astrocytes

In the brain, astrocytes represent the cellular population that expresses the highest amount of connexins (Cxs). This family of membrane proteins is the molecular constituent of gap junction channels and hemichannels that provide pathways for direct cytoplasm-to-cytoplasm and inside-out exchange, respectively. Both types of Cx channels are permeable to ions and small signaling molecules allowing astrocytes to establish dynamic interactions with neurons. So far, most pharmacological approaches currently available do not distinguish between these two channel functions, stressing the need to develop new specific molecular tools. In astrocytes two major Cxs are expressed, Cx43 and Cx30, and there is now evidence indicating that at least Cx43 operates as a gap junction channel as well as a hemichannel in these cells. Based on studies in primary cultures as well as in acute hippocampal slices, we report here that Gap 19, a nonapeptide derived from the cytoplasmic loop of Cx43, inhibits astroglial Cx43 hemichannels in a dose-dependent manner, without affecting gap junction channels. This peptide, which not only selectively inhibits hemichannels but is also specific for Cx43, can be delivered in vivo in mice as TAT-Gap19, and displays penetration into the brain parenchyma. As a result, Gap 19 combined with other tools opens up new avenues to decipher the role of Cx43 hemichannels in interactions between astrocytes and neurons in physiological as well as pathological situations