Carabid Beetles (Coleoptera: Carabidae) as Prey of North American Frogs

In this paper, 62 species of carabid beetles are listed as prey of frogs. Records are from the literature and a stomach contents study. During the period May to October 1973, I collected and examined the stomach contents of 2 bullfrogs, 45 green frogs, 504 northern leopard frogs and 7 wood frogs from southern Quebec in order to learn whether carabid beetles are major or minor prey of frogs

Specialist or generalist? Feeding ecology of the Malagasy poison frog Mantella aurantiaca

We studied the diet of a population of free-ranging Mantella aurantiaca, an alkaloid-containing poison frog from Madagascar. As in other poison frogs, this species is thought to sequester alkaloids from arthropod prey. Among prey, mites and ants are known to regularly contain alkaloids and mites appear to be a major source of dietary alkaloids in poison frogs. We predicted that mites and ants would constitute the most important prey item for these frogs. Prey inventories were obtained during the rainy season by stomach flushing 23 adult male and 42 adult female frogs from one population. Males had smaller body sizes than females and ate smaller prey items, but males and females displayed no differences in the number of prey items consumed. The numerical proportion of ants in most specimens was surprisingly low (11% in males and 15% in females), while mites were slightly more frequent (34% in males and 18% in females). Other prey items consumed in large proportions were flies and collembolans. Comparing the total of 5492 arthropod prey items with 1867 arthropods sampled from the frogs' leaf litter habitat, the proportion of prey classes did not significantly differ among the samples, indicating a low degree of prey electivity in this population. Our data suggest that not all poison frogs exhibit a continuous and active preference for feeding on ants and mites, but instead some may consume high proportions of ants due to a high abundance of ants in their environment

CALL BROADCASTING AND AUTOMATED RECORDERS AS TOOLS FOR ANURAN SURVEYS IN A SUBARCTIC TUNDRA LANDSCAPE

Relatively little is known about population ecology of anurans in arctic and subarctic tundra regions, in part because it is difficult to survey anurans in these landscapes. Anuran survey protocols developed for temperate regions have limited applicability in arctic and subarctic tundra landscapes, which may lack roads and vehicle access, and experience variable and inclement weather during short anuran breeding seasons. To evaluate approaches to address some of the limitations of surveying anurans in tundra landscapes, we assessed the effectiveness of using breeding call broadcasts to increase detection of Boreal Chorus Frogs (Pseudacris maculata) and Wood Frogs (Lithobates sylvaticus) near Cape Churchill, Manitoba, Canada. We also evaluated how counts of anurans derived from automated audio recorders compared with those obtained simultaneously by observers. We detected on average 0.4 additional Wood Frogs per survey when we broadcasted calls (x = 0.82, SD = 1.38), an increase of > 40% compared to surveys without broadcasts (x = 1.24, SD = 1.51; Wilcoxon test; Z = 2.73, P = 0.006). In contrast, broadcasting Boreal Chorus Frog calls did not increase the number of chorus frog detections (Wilcoxon test; Z < 0.001, P > 0.90). Detections of Wood Frogs in a 100-m radius were lower via automated recorders (x = 0.60, SD = 0.87 SD) than by observers during simultaneous surveys (x = 0.96, SD = 1.27 Z = 2.07, P = 0.038), but those of Boreal Chorus Frogs were not different (x = 1.72, SD = 1.31;x = 1.44, SD = 1.5; Z = 1.55, P > 0.121). Our results suggest that broadcasting calls can increase detection of Wood Frogs, and that automated recorders are useful in detecting both Wood Frogs and Boreal Chorus Fogs in arctic and subarctic tundra landscapes

Differential Uptake of Gold Nanoparticles by 2 Species of Tadpole, the Wood Frog (Lithobates Sylvaticus) and the Bullfrog (Lithobates Catesbeianus)

Engineered nanoparticles are aquatic contaminants of emerging concern that exert ecotoxicological effects on a wide variety of organisms. We exposed cetyltrimethylammonium bromide–capped spherical gold nanoparticles to wood frog and bullfrog tadpoles with conspecifics and in combination with the other species continuously for 21 d, then measured uptake and localization of gold. Wood frog tadpoles alone and in combination with bullfrog tadpoles took up significantly more gold than bullfrogs. Bullfrog tadpoles in combination with wood frogs took up significantly more gold than controls. The rank order of weight-normalized gold uptake was wood frogs in combination \u3e wood frogs alone \u3e bullfrogs in combination \u3e bullfrogs alone \u3e controls. In all gold-exposed groups of tadpoles, gold was concentrated in the anterior region compared with the posterior region of the body. The concentration of gold nanoparticles in the anterior region of wood frogs both alone and in combination with bullfrogs was significantly higher than the corresponding posterior regions. We also measured depuration time of gold in wood frogs. After 21 d in a solution of gold nanoparticles, tadpoles lost \u3e83% of internalized gold when placed in gold-free water for 5 d. After 10 d in gold-free water, tadpoles lost 94% of their gold. After 15 d, gold concentrations were below the level of detection. Our finding of differential uptake between closely related species living in similar habitats with overlapping geographical distributions argues against generalizing toxicological effects of nanoparticles for a large group of organisms based on measurements in only one species

Frog origins: inferences based on ancestral reconstructions of locomotor performance and anatomy

Frogs are the most species-rich and ecologically diverse group of amphibians and are characterized by a unique body plan including long legs, elongated ilia, and fused caudal vertebrae. Stem anurans such as Triadobatrachus or Czatkobatrachus have been suggested to have used jumping or hopping as part of their locomotor repertoire based on their anatomy. The earliest known true frog, Prosalirus bitis was suggested to have been a proficient jumper. However, data on jumping performance in frogs have never been used to attempt reconstruction of ancestral features at the base of the radiation. Here we provide data on jumping performance (forces and acceleration) in 20 species of extant frogs including representatives of most of the early radiating clades. We next use ancestral character value inferences to assess ancestral features. Our analyses suggest that frog ancestors were of small to medium size, had relatively short limbs, produced rather low jump forces, yet were capable of relatively high acceleration. Given the short limbs and low forces, the unique frog bauplan with a reduced vertebral column and a mobile ilio-sacral joint may not have been an adaptation for powerful jumping

Arboreal frogs, tank bromeliads and disturbed seasonal tropical forest

We investigated the relationship between arboreal frogs, tank bromeliads and landscape transformation in tropical forests of southeastern Campeche, Mexico. We surveyed frogs in six distinct habitats: slash and burn agriculture, seasonally flooded forest (bajo), aquatic habitats (lagoons and small ponds), second growth upland forest, primary forest and creek habitat using both systematic and non-systematic surveys. The highest species richness of frogs was documented in primary forest and small ponds. In contrast, no frogs were recorded in second growth forest. Similarly, tank bromeliads (Aechmea bracteata) were completely absent from early successional stages and were almost twice as abundant in seasonally flooded forest as in upland forest. The vertical distribution of A. bracteata differed between forest types, and they significantly more abundant in larger diameter trees. We examined 60 tank bromeliads during the peak of the dry season to test their use as refugia by frogs. Approximately 27% of tank bromeliads sampled had arboreal frogs belonging to three species, but 9 species have been recorded as occasional users of bromeliads in the region. There were significantly more frogs on large than on medium-sized bromeliads, and frogs were more abundant on bromeliads higher on host trees, particularly those above 3 m in height. Our results suggest that the loss of tank bromeliads from drier and less structurally complex habitats created by slash and burn agriculture and selective logging results in loss of refugia for arboreal frogs in this seasonal tropical forest. We suggest that Aechmea bracteata be a keystone species in seasonal tropical forest

CHORUSING PATTERNS OF A DIVERSE ANURAN COMMUNITY, WITH AN EMPHASIS ON SOUTHERN CRAWFISH FROGS (LITHOBATES AREOLATUS AREOLATUS)

Wildlife surveys have a critical role in conservation efforts and the collection of life history data. For anuran amphibians these surveys often focus on calling males. In order to further our understanding of anuran ecology, we used automated recording systems to monitor the calling activities of the anuran communities at two beaver-formed lakes and one cattle pond in southeastern Oklahoma. We documented 14 anuran species between 5 February and 28 April 2012. Temperature had a significant effect on the calling patterns of Eastern Narrow-mouthed Toads (Gastrophryne carolinensis), Green Treefrogs (Hyla cinerea), Gray Treefrogs (Hyla versicolor), Southern Crawfish Frogs (Lithobates areolatus areolatus), and Cajun Chorus Frogs (Pseudacris fouquettei). Temperature did not have a significant effect on the calling patterns of Dwarf American Toads (Anaxyrus americanus charlesmithi), American Bullfrogs (Lithobates catesbeianus), or Green Frogs (Lithobates clamitans). There was not a significant relationship between rainfall and calling for L. a. areolatus. The presence of several of these species, including L. a. areolatus and Hurter’s Spadefoots (Scaphiopus hurterii) was unusual because these anurans typically breed in ephemeral, fishless pools, but the beaver lakes are permanent and sustain populations of carnivorous fishes

Sticking under wet conditions: the remarkable attachment abilities of the torrent frog, staurois guttatus

Tree frogs climb smooth surfaces utilising capillary forces arising from an air-fluid interface around their toe pads, whereas torrent frogs are able to climb in wet environments near waterfalls where the integrity of the meniscus is at risk. This study compares the adhesive capabilities of a torrent frog to a tree frog, investigating possible adaptations for adhesion under wet conditions. We challenged both frog species to cling to a platform which could be tilted from the horizontal to an upside-down orientation, testing the frogs on different levels of roughness and water flow. On dry, smooth surfaces, both frog species stayed attached to overhanging slopes equally well. In contrast, under both low and high flow rate conditions, the torrent frogs performed significantly better, even adhering under conditions where their toe pads were submerged in water, abolishing the meniscus that underlies capillarity. Using a transparent platform where areas of contact are illuminated, we measured the contact area of frogs during platform rotation under dry conditions. Both frog species not only used the contact area of their pads to adhere, but also large parts of their belly and thigh skin. In the tree frogs, the belly and thighs often detached on steeper slopes, whereas the torrent frogs increased the use of these areas as the slope angle increased. Probing small areas of the different skin parts with a force transducer revealed that forces declined significantly in wet conditions, with only minor differences between the frog species. The superior abilities of the torrent frogs were thus due to the large contact area they used on steep, overhanging surfaces. SEM images revealed slightly elongated cells in the periphery of the toe pads in the torrent frogs, with straightened channels in between them which could facilitate drainage of excess fluid underneath the pad