1 research outputs found
The rusty plumage coloration of juvenile gyrfalcons is produced by pheomelanin and its expression is affected by an intracellular antioxidant
Juveniles of many diurnal raptors exhibit a characteristic rusty plumage coloration whose
biochemical basis has never been determined. Using the Gyrfalcon (Falco rusticolus) as a model species,
we analyzed feathers by Raman spectroscopy and showed that the rusty color is due to the presence of the
pigment pheomelanin, which was also observed in the feathers of a juvenile Peregrine Falcon (Falco
peregrinus). We experimentally modified the expression of the rusty plumage coloration by treating four
developing Gyrfalcons with buthionine sulfoximine (BSO), a specific and nontoxic inhibitor of glutathione
(GSH) synthesis. Because cysteine, one of the three constitutive amino acids of GSH, is required for
pheomelanin synthesis and GSH is the most important intracellular antioxidant, these findings indicate
that the expression of rusty plumage coloration can be affected by environmental oxidative stress. Our
results suggest that the rusty plumage coloration of at least some diurnal raptors is pheomelanin-based, and
the dependence on GSH levels opens the possibility that the evolution of this trait in some species and the
age-related variation in its expression across species may be explained by interspecific and intraspecific
variation in exposure to environmental factors that generate oxidative stress and by age-related variations in
endogenous levels of oxidative stressPeer reviewe