Review on carbon-derived, solid-state, micro and nano sensors for electrochemical sensing applications

The aim of this review is to summarize the most relevant contributions in the development of electrochemical sensors based on carbon materials in the recent years. There have been increasing numbers of reports on the first application of carbon derived materials for the preparation of an electrochemical sensor. These include carbon nanotubes, diamond like carbon films and diamond film-based sensors demonstrating that the particular structure of these carbon material and their unique properties make them a very attractive material for the design of electrochemical biosensors and gas sensors. Carbon nanotubes (CNT) have become one of the most extensively studied nanostructures because of their unique properties. CNT can enhance the electrochemical reactivity of important biomolecules and can promote the electron-transfer reactions of proteins (including those where the redox center is embedded deep within the glycoprotein shell). In addition to enhanced electrochemical reactivity, CNT-modified electrodes have been shown useful to be coated with biomolecules (e.g., nucleic acids) and to alleviate surface fouling effects (such as those involved in the NADH oxidation process). The remarkable sensitivity of CNT conductivity with the surface adsorbates permits the use of CNT as highly sensitive nanoscale sensors. These properties make CNT extremely attractive for a wide range of electrochemical sensors ranging from amperometric enzyme electrodes to DNA hybridization biosensors. Recently, a CNT sensor based fast diagnosis method using non-treated blood assay has been developed for specific detection of hepatitis B virus (HBV) (human liver diseases, such as chronic hepatitis, cirrhosis, and hepatocellular carcinoma caused by hepatitis B virus). The linear detection limits for HBV plasma is in the range 0.5–3.0 μL−1 and for anti- HBVs 0.035–0.242 mg/mL in a 0.1 M NH4H2PO4 electrolyte solution. These detection limits enables early detection of HBV infection in suspected serum samples. Therefore, non-treated blood serum can be directly applied for real-time sensitive detection in medical diagnosis as well as in direct in vivo monitoring. Synthetic diamond has been recognized as an extremely attractive material for both (bio-) chemical sensing and as an interface to biological systems. Synthetic diamond have outstanding electrochemical properties, superior chemical inertness and biocompatibility. Recent advances in the synthesis of highly conducting nanocrystalline-diamond thin films and nano wires have lead to an entirely new class of electrochemical biosensors and bio-inorganic interfaces. In addition, it also combines with development of new chemical approaches to covalently attach biomolecules on the diamond surface also contributed to the advancement of diamond-based biosensors. The feasibility of a capacitive field-effect EDIS (electrolyte-diamond-insulatorsemiconductor) platform for multi-parameter sensing is demonstrated with an O-terminated nanocrystalline-diamond (NCD) film as transducer material for the detection of pH and penicillin concentration. This has also been extended for the label-free electrical monitoring of adsorption and binding of charged macromolecules. One more recent study demonstrated a novel bio-sensing platform, which is introduced by combination of a) geometrically controlled DNA bonding using vertically aligned diamond nano-wires and b) the superior electrochemical sensing properties of diamond as transducer material. Diamond nanowires can be a new approach towards next generation electrochemical gene sensor platforms. This review highlights the advantages of these carbon materials to promote different electron transfer reactions specially those related to biomolecules. Different strategies have been applied for constructing carbon material-based electrochemical sensors, their analytical performance and future prospects are discussed

Tumor derived Microvesicles enhance cross-processing ability of clinical grade Dendritic Cells

Tumor cells release extracellular microvesicles (MVs) in the microenvironment to deliver biological signals to neighbouring cells as well as to cells in distant tissues. Tumor-derived MVs appear to play contradictory role promoting both immunosuppression and tumor growth and both evoking tumor specific immune response. Recent evidences indicate that tumor-derived MVs can positively impact Dendritic Cells (DCs) immunogenicity by reprogramming DC antigen processing machinery and intracellular signaling pathways, thus promoting anti-tumor response. DCs are considered pivot cells of the immune system due to their exclusive ability to coordinate the innate and acquired immune responses, cross-present exogenous antigens and prime naïve T cells. DCs are required for the induction and maintenance of long-lasting anti-tumor immunity and their exploitation has been extensively investigated for the design of anti-tumor vaccines. However, the clinical grade culture conditions that are required to generate DCs for therapeutic use can strongly affect their functions. Here, we investigated the immunomodulatory impact of MVs carrying the MUC1 tumor glycoantigen (MVsMUC1) as immunogen formulation on clinical grade DCs grown in X-VIVO 15 (X-DCs). Results indicated that X-DCs displayed reduced performance of the antigen processing machinery in term of diminished phagocytosis and acidification of the phagosomal compartment suggesting an altered immunogenicity of clinical grade DCs. Pulsing DCs with MVsMUC1 restored phagosomal alkalinization, triggering ROS increase. This was not observed when a soluble MUC1 protein was employed (rMUC1). Concurrently, MVsMUC1 internalization by X-DCs allowed MUC1 cross-processing. Most importantly, MVsMUC1 pulsed DCs activated IFNγ response mediated by MUC1 specific CD8+ T cells. These results strongly support the employment of tumor-derived MVs as immunogen platforms for the implementation of DC-based vaccine

Derived moduli of complexes and derived Grassmannians

In the first part of this paper we construct a model structure for the category of filtered cochain complexes of modules over some commutative ring $R$ and explain how the classical Rees construction relates this to the usual projective model structure over cochain complexes. The second part of the paper is devoted to the study of derived moduli of sheaves: we give a new proof of the representability of the derived stack of perfect complexes over a proper scheme and then use the new model structure for filtered complexes to tackle moduli of filtered derived modules. As an application, we construct derived versions of Grassmannians and flag varieties.Comment: 54 pages, Section 2.4 significantly extended, minor corrections to the rest of the pape

Gut-Derived Serum Lipopolysaccharide is Associated With Enhanced Risk of Major Adverse Cardiovascular Events in Atrial Fibrillation: Effect of Adherence to Mediterranean Diet

Gut microbiota is emerging as a novel risk factor for atherothrombosis, but the predictive role of gut-derived lipopolysaccharide (LPS) is unknown. We analyzed (1) the association between LPS and major adverse cardiovascular events (MACE) in atrial fibrillation (AF) and (2) its relationship with adherence to a Mediterranean diet (Med-diet)

Derived Smooth Manifolds

We define a simplicial category called the category of derived manifolds. It contains the category of smooth manifolds as a full discrete subcategory, and it is closed under taking arbitrary intersections in a manifold. A derived manifold is a space together with a sheaf of local $C^\infty$-rings that is obtained by patching together homotopy zero-sets of smooth functions on Euclidean spaces. We show that derived manifolds come equipped with a stable normal bundle and can be imbedded into Euclidean space. We define a cohomology theory called derived cobordism, and use a Pontrjagin-Thom argument to show that the derived cobordism theory is isomorphic to the classical cobordism theory. This allows us to define fundamental classes in cobordism for all derived manifolds. In particular, the intersection $A\cap B$ of submanifolds $A,B\subset X$ exists on the categorical level in our theory, and a cup product formula $$[A]\smile[B]=[A\cap B]$$ holds, even if the submanifolds are not transverse. One can thus consider the theory of derived manifolds as a {\em categorification} of intersection theory.Comment: 57 pages. Reformulation of author's PhD thesis. To appear in Duke Math J

Derived Algebraic Geometry

This text is a survey of derived algebraic geometry. It covers a variety of general notions and results from the subject with a view on the recent developments at the interface with deformation quantization.Comment: Final version. To appear in EMS Surveys in Mathematical Science

Derived Quot schemes

Realizing a part of the Derived Deformation Theory program, we construct a "derived" analog of the Grothendieck's Quot scheme parametrizing subsheaves in a given coherent sheaf F on a smooth projective variety X. This analog is a differential graded manifold RQuot_h(F) (so it is always smooth in an appropriate sense) whose tangent space at a point represented by a subsheaf K in F, is a cochain complex quasiisomorphic to RHom(K, F/K).Comment: 46 pages, AMS-TeX. Revised version, to appear in Ann. Sci. EN