Feasibility of Using Cryostored Colorado Potato Beetle (Coleoptera: Chrysomelidae) Eggs for Rearing \u3ci\u3eEdovum Puttleri\u3c/i\u3e (Hymenoptera: Eulophidae)

Cryostored eggs of Colorado potato beetle, Leptinotarsa decemlineata (Say) eggs were suitable hosts for Edovum puttleri. Parasitism rates were low, however this study was first to show that rearing the parasite on eggs stored at ultra low temperatures (-70 0 C) is feasible. This method of storage will facilitate mass production of E. puttleri as well as prevent loss of the parasite in the event of a crash in the Colorado potato beetle colony

Control of the Colorado Potato Beetle (Coleoptera: Chrysomelidae) on Tomatoes with \u3ci\u3eBacillus Thuringiensis\u3c/i\u3e Var. \u3ci\u3eThuringiensis\u3c/i\u3e

(excerpt) The Colorado potato beetle, Leptinotarsa decemlineata (Say), is a serious pest of tomatoes grown in this country. This beetle is also developing resistance to several classes of chemical insecticides including most of the carbamates, chlorinated hydrocarbons, and organophosphates (Forgash 1981). and most recently to the pyrethroids. According to figures in a report released by Schwartz and Klassen (1981). the value of the tomato crop in the CSA in 1978 was placed at $914,121,000 and crop loss due to Colorado potato beetle (CPB) damage alone would be 93% of its value if no control were undertaken. These figures along with the fact that the CPB is developing resistance dictate the need for. and development of, alternative control measures

ALTERNTIVE PROTECTION OF BIOPOTATOES IN ORGANIC FARMING

There were tested water extracts from Syringa vulgaris and Pyrethrum parthenium. They did not prove significant differences of decrease of the air attack of Colorado potato beetle (Leptinotarsa decemlineata), the activity of eggs – laying and the occurrence of larvae in comparison to the control. Interesting trends of lower-eggs lying and a lower larvae occurrence were discovered. The liquid spray against the late blight (Phytophthora infestans) did not significantly decrease neither occurrence of late blight or the yield. The production of the bio-potatoes is only 0,2 % of all potatoes production in the Czech Republic. The aim of this experiment was to try alternative methods of regulation of Colorado potato beetle and late blight (the biggest harmfull organisms of potatoes). For biological agriculture could be also suitable water extracts. These are possible to use as protection of potatoes against unfavorable factors (Colorado potate beetle and late blight) and increase yield and quality of production

Optimierte Anwendung von Pflanzenschutzmitteln zur Regulierung des Kartoffelkäfers (Leptinotarsa decemlineata Say) im Ökologischen Landbau

The Colorado Potato Beetle is one of the most important pests of potatoes. The effect of various plant protection products based on neem (NeemAzal-T/S), pyrethrum/rape oil (Spruzit Neu) and Bacillus thuringiensis - B.t.t. (Novodor FC) against this pest has been compared in a field experiment from 2004 until 2006. The combined application of neem and B.t.t.-product have reduced the number of Colorado Potato Beetle larvae as well as the losses of plant material by feed of the beetle larvae significantly. In three years of field experiments the application of pyrethrum/rape oil has shown no significant effect to reduce the number of Colorado Potato Beetles

Alternative protection of potatoes in organic farming

There were tested water extracts from Syringa vulgaris and Pyrethrum parthenium. They did not prove signincant differences ofdecrease of the air attack ofColorado potato beetle (Leptinotarsa decemlineata), the activity ofeggs -laying and the occurrence oflarvae in comparison to the control. Interesting trends oflower-eggs lying and a lower larvae occurrence were discovered. The liquid spray against the late blight (Phytophthora infestans) did not signincantly decrease neither occurrence oflate blight or the yield. The production of the bio-potatoes is only 0,2% of all potatoes production in the Czech Republic. The aim of this experiment was to try alternative methods of regulation of Colorado potato beetle and late blight (the biggest harmful! organisms ofpotatoes). For biological agriculture could be also suitable water extracts. These are possible to use as protection of potatoes against unfavorable factors (Colorado potate beetle and late blight) and increase yield and quality ofproduction

The Influence of Plant Dispersion on Movement Patterns of the Colorado Potato Beetle, \u3ci\u3eLeptinotarsa Decemlineata\u3c/i\u3e (Coleoptera: Chrysomelidae)

The influence of plant dispersion on movements of the Colorado potato beetle, Leptinotarsa decemlineata (Say) (Coleoptera: Chrysomelidae), was studied with mark-recapture techniques. Beetles released between potato monocultures, polycultures with two additional non-host plant species, and polycultures with five additional non-host species, randomly colonized the three types of plots. Releases between different arrangements of potted host and non-host plants showed (1) greater beetle colonization and greater length of time spent on potato plants growing alone than on potato plants surrounded by non-host vegetation, and (2) no effect of potato plant density on colonization or tenure time. Overall, there was a 65~ recapture rate; beetles consistently stayed on the same plants they originally colonized, often for at least five days after release

Vergleichende Untersuchungen zur Regulierung des Kartoffelkäfers (Leptinotarsa decemlineata Say) mit Pyrethrum/Rapsöl-, Neemöl- und Bacillus thuringiensis-Präparaten

The Colorado Potato Beetle is one of the most important pests of potatoes. The effect of various plant protection products with neem oil (NeemAzal-T/S), pyrethrum/rape oil (Spruzit Neu) and Bacillus thuringiensis-B.t.t.(Novodor Neu) against this pest was compared in a field experiment 2004. Neem oil and B.t.t. reduced the number of Colorado Potato Beetle larvae as well as the losses of plant material due to feeding by beetle larvae significantly in the same way. Beneficial arthropods were not affected. The application of pyrethrum/rape oil has shown in this experiment no significant effect to reduce the number of Colorado Potato Beetles. However, the number of beneficial arthropods was reduced from 2.2 individuals/plant (untreated control) to 1.0 individual/plant

W208-Vegetable Pests - Colorado Potato Beetle

The Colorado potato beetle, Leptinotarsa decemlineata (Say), is found throughout most of the United States and Mexico and has been introduced into Europe and parts of Asia. Mexico is considered the center of origin for this pest. The Colorado potato beetle is an important pest of Solanaceous plants. Potato is the preferred host, but this insect pest also will feed on tomato, eggplant, pepper, tobacco, ground cherry, horse-nettle, nightshade, belladonna, thorn apple, henbane, buffalobur and other related plant species

EC86-1546 Common Vegetable Insects

This 4-color extension circular was prepared by Extension entomologists of the North Central States in cooperation with the Federal Extension Service, U.S. Department of Agriculture. This publication shows the following insects: 1. Cabbage looper and imported cabbageworm 2. Cabbage aphid 3. Hornworm 4. Two-spotted spider mite 5. Bean leaf beetle 6. Mexican bean beetle adult, pupa, larvae, eggs 7. Thrips 8. Root maggot 9. Striped cucumber beetle 10. Spotted cucumber beetle 11. Colorado potato beetle 12. Potato flea beetle 13. Potato leafhopper 14. Squash vine borer 15. Squash bug nymphs and adult

The Characterization of Carbamate Resistance in the Colorado Potato Beetle, \u3cem\u3eLeptinotarsa decemlineata\u3c/em\u3e (Say), in Comparison with the House Fly, \u3cem\u3eMusca domestica\u3c/em\u3e (L)

Resistant and susceptible populations of the Colorado potato beetle, Leptinotarsa decemlineata (Say), and the house fly, Musca domestica (L), were compared with respect to carbofuran and carbaryl toxicity in the presence and absence of the synergist, piperonyl butoxide. Resistance levels of the New Jersey population when compared with the susceptible Logan population by topical application of carbaryl and carbofuran were \u3e 833 and 820, respectively. A resistance level of 583 was determined from carbofuran bioassays of Rutgers and NAIDM house flies. Similar levels of resistance development between these species suggests the possibility that similar resistance mechanisms may be involved. Utilization of the synergist difference approach for evaluating synergism of these carbamates by piperonyl butoxide indicated that the resistant strains depended to a much greater extent upon detoxication by monooxygenases than did their susceptible counterparts. While piperonyl butoxide synergism resulted in completely restoring the the Rutgers strain of house flies to levels of susceptible counterparts. While piperonyl butoxide synergism resulted in completely restoring the Rutgers strain of house flies to levels of susceptibility, New Jersey Colorado potato beetles were able to retain a significant portion of their resistance and to investigate the possibility that decreased absorption was responsible for the degree of resistance retained following piperonyl butoxide pretreatment. Microsomal preparations from Colorado potato beetle gut and fats body were devoid of measurable monooxygenase activity as determined from O-demethylation of p-nitroanisole, in spite of attempts clear gut contents and optimize techniques. In contrast, microsomal preparations from Rutgers and NAIDM house flies demonstrated clear differences in oxidative potential between strains. An analysis of the distribution of NAHPH-cytochrome c reductase in Colorado potato beetle microsomes revealed a loss of enzyme activity from the microsomal pellet into the soluble fraction. The solubilization of this component of the electron transport chain is suggested as a possible limiting factor for in vitro characterizations of the involvement of cytochrome P-450 in xenobiotic metabolism. Determination of NADPH oxidation from microsomal preparations from house flies abdomens and Colorado potato beetle gut and fat body did not demonstrate quantitative differences between tissue sources nor populations of either species. In a similar manner, NADPH-cytochrome c reductase did not vary between house fly strains nor between tissue sources of Colorado potato beetle. There was, however, approximately a two-fold difference in NADPH-cytochrome c reductase activity between resistant and susceptible populations of Colorado potato beetle larvae. The in vivo distribution of 1-naphthyl-N-methyl (14C) carbamate in resistant and susceptible Colorado potato beetle larvae demonstrated that although significant quantitative differences did not exist between populations with respect to the rate of penetration, excretion, of the radiocarbon was significantly greater in the resistant New Jersey population. This study has been successful in establishing that monooxygenases play a chief role in Colorado potato beetle resistance to carbamate insecticides. This role in Colorado potato beetle resistance to carbamate insecticides. This role was confirmed in part by an increased rate of NADPH-cytochrome c reductase activity in the resistant population, however, traditional xenobiotic metabolism could not be confirmed by other methodology examined. This may be a result of the apparent solubilization of NADPH cytochrome P-450 reductase from microsmal preparations due to the unconfirmed presence of an endogenous inhibitor. Further characterizations of resistance mechanisms need to be examined for this destructive agricultural pest