Computation of the Binding Energies in the Inverse Problem Framework

We formalized the nuclear mass problem in the inverse problem framework. This approach allows us to infer the underlying model parameters from experimental observation, rather than to predict the observations from the model parameters. The inverse problem was formulated for the numericaly generalized the semi-empirical mass formula of Bethe and von Weizs\"{a}cker. It was solved in step by step way based on the AME2012 nuclear database. The solution of the overdetermined system of nonlinear equations has been obtained with the help of the Aleksandrov's auto-regularization method of Gauss-Newton type for ill-posed problems. In the obtained generalized model the corrections to the binding energy depend on nine proton (2, 8, 14, 20, 28, 50, 82, 108, 124) and ten neutron (2, 8, 14, 20, 28, 50, 82, 124, 152, 202) magic numbers as well on the asymptotic boundaries of their influence. These results help us to evaluate the borders of the nuclear landscape and show their limit. The efficiency of the applied approach was checked by comparing relevant results with the results obtained independently.Comment: 9 pages, 1 figure, Proceedings of the International Symposium on Exotic Nuclei EXON-2016, Kazan, Russia, 4-10 September 2016. based on arXiv:1602.0677

Simultaneously non-linear energy calibration of CMS calorimeters for single pions and electrons

CMS calorimeter energy calibration was done in the full CMS simulated geometry for the pseudorapidity region eta = 0. The samples of single pion events were generated with a set of incident energies from 5 GeV to 3 TeV and for single electrons from 5 to 500 GeV. The analysis of the simulated data shows that standard calibration using just sampling coefficients for calorimeter parts with different sampling ratio gives nonlinear calorimeter response. Non-linear calibration technique was applied simultaneously for pion and electron beams which is preparation for jets energy reconstruction. It improve calorimeter energy resolution for pions and restore the calorimeter linearity.Comment: 7 pages, 2 figures, latex fil

Chemotherapy accelerates immune-senescence and functional impairments of Vδ2pos T cells in elderly patients affected by liver metastatic colorectal cancer.

Human (gamma delta) γδ T cells are unconventional innate-like lymphocytes displaying a broad array of anti-tumor activities with promising perspectives in cancer immunotherapy. In this context, Vδ2pos T cells represent the preferential target of several immunotherapy protocols against solid tumors. However, the impact of both aging and chemotherapy (CHT) on Vδ2pos T cells is still unknown. The present study evaluates with multi-parametric flow cytometry the frequencies, terminal differentiation, senescence and effector-functions of peripheral blood and tumor infiltrating Vδ2pos T cells purified from liver metastases (CLM) of patients affected by colorectal cancer (CRC) compared to those of sex- and age-matched healthy donors. The peripheral blood of CLM patients underwent CHT is characterized by decreased amounts of Vδ2pos T cells showing a relative increase of terminally-differentiated CD27neg/CD45RApos (TEMRA) cells. The enrichment of this latter subset is associated with an increased expression of the senescent marker CD57. The acquisition of CD57 on TEMRA Vδ2pos T cells is also coupled with impairments in cytotoxicity and production of TNF-α and IFN-γ. These features resemble the acquisition of an immune-senescent profile by Vδ2pos T cells from CLM patients that received CHT, a phenomenon that is also associated with the loss of the co-stimulatory marker CD28 and with the induced expression of CD16. The group of CLM patients underwent CHT and older than 60 years old showed higher frequencies of CD57pos and TEMRA Vδ2pos T cells. Similar results were found for tumor infiltrating Vδ2pos T cell subset purified from CLM specimens of patients treated with CHT. The toxicity of CHT regimens also affects the homeostasis of Vδ2pos T cells by inducing higher frequencies of circulating CD57pos TEMRA subset in CLM underwent CHT and younger than 60 years old. Taken together, our data demonstrate that the enrichment of senescent Vδ2pos T cells in CLM patients is not only induced by patients' aging but also by the toxicity of CHT that further accelerates the accumulation of CD57pos TEMRA cells highly dysfunctional in their anti-tumor activities. These results are important to both predict the clinical outcome of CLM and to optimize those protocols of cell cancer immunotherapy employing unconventional Vδ2pos T cells