Micellar electrokinetic capillary chromatography—Synchronous monitoring of substrate and products in the myrosinase catalysed hydrolysis of glucosinolates

A micellar electrokinetic capillary chromatography (MECC) method has been developed for monitoring the myrosinase catalysed hydrolysis of 2-hydroxy substituted glucosinolates and the simultaneous formation of the corresponding degradation products (oxazolidine-2-thiones (OZTs) and nitriles). Glucosibarin ((2R)-2-hydroxy-2-phenylethylglucosinolate) was chosen as the model glucosinolate owing to the difficulties in determining hydrolysis rates of this type of substrates in traditional UV-assays. The method was afterwards validated with glucobarbarin ((2S)-2-hydroxy-2-phenylethylglucosinolate) and progoitrin ((2R)-2-hydroxybut-3-enylglucosinolate). Aromatic glucosinolates without a 2-hydroxy group in their side chains, such as glucotropaeolin (benzylglucosinolate) and gluconasturtiin (phenethylglucosinolate) were also tested. Formation of the glucosinolate hydrolysis products was monitored simultaneously at 206 nm and 230 nm. This allowed estimation of the extinction coefficient of the OZT derived from glucosibarin, which was found to be 18,000M−1 cm−1 and 12,000M−1 cm−1 at 206 nm and 230 nm, respectively. The developed method has limit of detection of 0.04mM and 0.06mM and limit of quantification of 0.2mM and 0.3mM for the glucosibarin derived OZT and nitrile, respectively. Linearity of the glucosinolate concentration was examined at six concentration levels from 2.5mMto 100mMand at 206 nm a straight line (R2 = 0.9996) was obtained. The number of theoretical plates (N) at the optimal system conditions was 245,000 for the intact glucosibarin, 264,000 for the OZT and 252,000 for the nitrile

Effect of ascorbic acid and glutathione on the production of nitriles by myrosinase

Biofumigation is based on the use of glucosinolate-containing plants for the control of soil-borne pest and diseases. Upon tissue damage, glucosinolates are hydrolyzed by endogenous enzymes (myrosinase) and a range of biologically active compounds are formed. Isothiocyanates (ITCs) are the quantitatively dominating products formed at neutral pH. Most of these compounds are volatile and only sparingly soluble in aqueous systems, and depending on the R-group structure and the presence of nucleophiles, further transformation of ITCs occurs. At lower pH and in the presence of certain molecules able to deliver two redox equivalents, the proportion of nitriles increases at the expense of ITC. The effect of ascorbic acid and glutathione on the production of nitriles at pH 5 was investigated by micellar electrokinetic capillary chromatography (MECC). The presence of 0.25 µmol ascorbic acid increased the production of nitriles although at higher concentrations the proportion of nitriles decreased. Increasing amounts of GSH favored the production of nitriles (40% of the total degradation products were nitriles in the presence of 2 µmol GSH). The oxidation of GSH gives the redox equivalents needed for the liberation of the sulfur from the unstable intermediate of the glucosinolate hydrolysis leading to the formation of the nitrile

Elliptic Flow: A Study of Space-Momentum Correlations In Relativistic Nuclear Collisions

Here I review measurements of v2, the second component in a Fourier decomposition of the azimuthal dependence of particle production relative to the reaction plane in heavy-ion collisions. v2 is an observable central to the interpretation of the subsequent expansion of heavy-ion collisions. Its large value indicates significant space-momentum correlations, consistent with the rapid expansion of a strongly interacting Quark Gluon Plasma. Data is reviewed for collision energies from sqrt[sNN] = 2 to 200 GeV. Scaling observations and comparisons to hydrodynamic models are discussed.Comment: 53 pages, 37 figures, a review article written for the QGP4 book edited by Rudy Hwa and Xin-Nian Wang (more minor updates, typos, and updated references

Identified particle measurements at RHIC: elucidating hadronization mechanisms for bulk partonic matter

Measurements of identified particle momentum spectra at center of mass energy 200 GeV are reviewed. Emphasis is placed on the azimuthal dependence and the centrality dependence of hadron yields at intermediate transverse momentum (1.5 < p_T < 5 GeV/c). The first measurements of the fourth harmonic term (v_4) in the azimuthal variation of identified particle yields are shown. The recombination mechanism of hadron formation provides a consistent description of the dependence of these measurements on particle-type.Comment: 6 pages, 3 figures, Proceedings for the 20th Winter Workshop on Nuclear Dynamics, Trelawny Beach, Jamaica, March 15-20, 200

Literature Review of the Definition, Size and Turnover of the Creative Industries and Micro-Businesses in Scotland: Preliminary Research

This research seeks to review, evaluate and clarify the findings of five recent reports in relation to the discussion paper Creative Industries in Scotland. Micro-businesses, Access to Finance and the Public Purse by Bob Last for the Cultural Enterprise Office

RHIC Critical Point Search: Assessing STAR's Capabilities

In this report we discuss the capabilities and limitations of the STAR detector to search for signatures of the QCD critical point in a low energy scan at RHIC. We find that a RHIC low energy scan will cover a broad region of interest in the nuclear matter phase diagram and that the STAR detector -- a detector designed to measure the quantities that will be of interest in this search -- will provide new observables and improve on previous measurements in this energy range.Comment: 9 pages, 6 figures, proceedings for "The 3rd edition of the International Workshop - The Critical Point and Onset of Deconfinement" - July 3-7 2006 Galileo Galilei Institute, Florence, Ital

Glucosinolate hydrolysis products for weed control

Glucosinolates are allelochemicals present in all Brassica plants. Upon hydrolysis by endogenous enzymes they produce a series of biologically active compounds, such as isothiocyanates and their deriva-tives among others. These compounds have marked fungicidal, nematocidal and herbicidal effects and therefore their use as biodegradable natural products for crop protection has attracted much attention in the last years. A number of these compounds, either individually or in combination, were tested against Sinapis alba and Lollium perenne in Petri dishes bio-assays. C50 values as low as 0.7 and 0.2 mM were obtained. This may open the possibility for using glucosinolate hydrolysis products as herbicides