986 research outputs found

    Veteran Perspectives on Phantom Limb Pain and Non-Drug Interventions

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    Tonya L Rich,1,2 Hannah L Phelan,1,3 Amy A Gravely,1 Kierra J Falbo,1,2 Jacob A Finn,1,4 Mary E Matsumoto,1,5 Katherine J Muschler,1 Christine M Olney,1,2,6 Jessica E Kiecker,1 Andrew H Hansen1,2,7 1Minneapolis Veteran’s Affairs Health Care System, Minneapolis, MN, USA; 2Department of Rehabilitation Medicine, Division of Rehabilitation Science, University of Minnesota, Minneapolis, MN, USA; 3Medical College of Wisconsin, Milwaukee, WI, USA; 4Department of Psychiatry, University of Minnesota, Minneapolis, MN, USA; 5Department of Rehabilitation Medicine, University of Minnesota, Minneapolis, MN, USA; 6Department of Nursing, University of Minnesota, Minneapolis, MN, USA; 7Department of Biomedical Engineering, University of Minnesota, Minneapolis, MN, USACorrespondence: Tonya L Rich, Tel +1 612-467-5463, Fax +1 612-725-2093, Email [email protected]: Phantom limb pain (PLP) commonly occurs post-amputation and can negatively affect the daily functioning of persons with amputation. Best practices for medication and non-drug management remain unclear.Objective: To better understand the PLP experience and patients’ familiarity with treatments, phone interviews were conducted at the Minneapolis Veterans Affairs Regional Amputation Center in Veterans with amputations.Methods: Fifty Veteran participants (average age 66, 96% male) with lower limb amputation were recruited for phone-based data collection of patient-reported outcomes (ie, demographics using the Trinity Amputation and Prosthesis Experience Scales-Revised (TAPES-R) and pain experience using the Phantom Phenomena Questionnaire) to characterize the population and a semi-structured interview. Notes taken during interviews were analyzed using the Krueger and Casey constant comparison analysis method.Results: Participants had an average of 15 years since amputation, and 80% reported PLP as identified with the Phantom Phenomena Questionnaire. Investigators identified several core themes from the qualitative interviews including 1) high variability in the experience of PLP, 2) acceptance and resilience, and 3) PLP treatment perceptions. The majority of participants reported trying common non-drug treatments with none endorsed consistently as highly effective.Conclusion: More research is needed to inform identification and implementation of clinical best practices for non-drug interventions for PLP and understand the factors that influence engagement in non-drug interventions. The participants in this study were largely male, so these results may not be generalizable to females.Keywords: amputation, phantom limb pain, rehabilitation, non-drug interventions, pai

    Cytocentric measurement for regenerative medicine

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    Any Regenerative Medicine (RM) business requires reliably predictable cell and tissue products. Regulatory agencies expect control and documentation. However, laboratory tissue production is currently not predictable or well-controlled. Before conditions can be controlled to meet the needs of cells and tissues in culture for RM, we have to know what those needs are and be able to quantify them. Therefore, identification and measurement of critical cell quality attributes at a cellular or pericellular level is essential to generating reproducible cell and tissue products. Here, we identify some of the critical cell and process parameters for cell and tissue products as well as technologies available for sensing them. We also discuss available and needed technologies for monitoring both 2D and 3D cultures to manufacture reliable cell and tissue products for clinical and non-clinical use. As any industry matures, it improves and standardizes the quality of its products. Cytocentric measurement of cell and tissue quality attributes are needed for RM

    Process: Calypso from Page to Performance

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    Details the process of writing and directing my capstone Calypso, from the inspirations of the project, the writing and rewriting of the many drafts along the way, the specific steps of the directing process, tech and performances of the play, and a reflection on what worked and what I could learn from

    Descripción agronómica y matemática de algunos sistemas de poda de Coffea arabica en Centro América, México y República Dominicana

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    This manual is aimed at agronomists, researchers and extensionists dedicated to the development of new ways of managing the vegetative and productive tissue of coffee through pruning. Here, we provide a technical-mathematical tool that helps in the conceptualization of the development of tissues subjected to pruning and its relationship with the yield of coffee beans, per plant and per area. In this manual we concentrate on the demographic and productive aspects of coffee plants and briefly present the mathematical foundations behind the models, which are presented in another publication (Somarriba et al. 2021)

    Epigenetic Regulation of Neural Transmission after Cerebellar Fastigial Nucleus Lesions in Juvenile Rats

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    Structural and functional abnormalities in the cerebellar midline region, including the fastigial nucleus, have been reported in neuropsychiatric disorders, also comprising the cerebellar cognitive affecting syndrome. In rats, early fastigial lesions reduce social interaction during development and lead to cognitive and emotional deficits in adults, accompanied by compromised neuronal network activity. Since epigenetic mechanisms are implicated in the etiology of neuropsychiatric disorders, we investigated whether fastigial nucleus lesions in juvenile rats would impact epigenetic regulation of neural transmission. The fastigial nucleus was lesioned bilaterally in 23-day-old male rats. Sham-lesion and naïve rats served as controls. DNA methylation was investigated for target genes of the GABAergic, dopaminergic, glutamatergic and oxytocinergic systems in brain regions with anatomic connections to the fastigial nucleus, i.e., medial prefrontal cortex, nucleus accumbens, striatum, thalamus, and sensorimotor cortex. Protein expression was examined for the respective target genes in case of altered DNA methylation between lesion and control groups. Lesioning of the fastigial nucleus led to significant differences in the epigenetic regulation of glutamate decarboxylase 1 and the oxytocin receptor in the nucleus accumbens and the prefrontal cortex. No differences were found for the other target genes and brain regions. Our findings indicate that epigenetic dysregulation after lesioning of the fastigial nucleus may influence long-term recovery and the emergence of behavioral changes. Together with previous behavioral and electrophysiological investigations of this rat model, these observations can play a role in the cerebellar cognitive affective syndrome and other neuropsychiatric disorders

    Tropical agriculture in Latin America and the Caribbean at the United Nations Food Systems Summit

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    Se presentan opciones para lograr la transformación y la optimización de algunos sistemas productivos tropicales a 2030 y 2050, partiendo de las capacidades disponibles en la región, de los avances en ciencia y tecnología y de los nuevos determinantes que enfrenta y enfrentará la franja intertropical. Se prioriza la transformación, como ejemplo práctico y académico, de dos sistemas productivos y agroalimentarios de relevancia en la región, pero de importancia particular en la franja intertropical: la agricultura industrial intensiva y la agricultura familiar.Options are presented to achieve the transformation and optimization of some tropical production systems by 2030 and 2050, based on the capacities available in the region, advances in science and technology, and the new determinants that the intertropical belt faces and will face. The transformation is prioritized, as a practical and academic example, of two productive and agri-food systems of relevance in the region, but of particular importance in the intertropical strip: the intensive industrial agriculture and family farming

    A comprehensive dataset of histopathology images, grades and patient demographics for human Osteoarthritis Cartilage

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    Osteoarthritis (OA) is a leading cause of disability in older adults and takes substantial toll at personal, economic and societal levels. There is inadequate comprehension of OA disease progression specifically during the early phases of OA. This knowledge is critical to understanding the heterogeneity in OA progression as well as enable development of targeted therapeutics at the start of the disease rather than end-stage. Histopathology of cartilage is a common method used to assess in situ state of cartilage tissue. The data presented in this article assesses the histopathological status of human cartilage specimens collected from 90 patients (n = 180). Each specimen was processed for histology and stained with hematoxylin and eosin (HE) and safranin O fast-green (SafO) for acquiring brightfield images to visualize changes in cartilage structure, cells, gycosaminoglycan content and tidemark integrity. The unstained sections were imaged using polarized light microscopy (PLM) to visualize changes in collagen organization and composition within the cartilage specimen. All the specimens were systematically graded by three scorers using established primary OA cartilage grading systems including Histological–Histochemical Grading System (HHGS), advanced Osteoarthritis Research Society International (OARSI) system and Polarized Light Microscopy (PLM) scoring system. These data can be used by the OA community as an educational resource to train new reviewers (scorers), it serves as a comprehensive image database for experienced OA community to review the wide spectrum of histopathological features presented by these mild to moderate OA specimens, to define different OA-subtypes, and to generate hypothesis on OA progression mechanisms. Finally, the high quality images can be used to develop machine learning algorithms for classification of OA, automated detection and segmentation of existing or new OA features that can serve as early OA histopathological indicators

    Acetabular Bone Marrow Aspiration During Total Hip Arthroplasty

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    Biologically augmented surgical treatments of orthopaedic conditions are increasingly popular. Bone marrow aspirate concentrate is a key orthobiologic tissue source, and the field is moving from the standard iliac crest marrow aspiration toward local aspirations of marrow depots that are accessible during the standard-of-care procedures in an attempt to reduce morbidity, surgery time, and cost. Here, we present the aspiration of the standard iliac marrow depot, but through a novel acetabular approach during total hip arthroplasty. This procedure markedly simplifies biologic augmentation with bone marrow aspirate concentrate in this large patient cohort

    Resorbable additively manufactured scaffold imparts dimensional stability to extraskeletally regenerated bone

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    Dimensionally stable vertical bone regeneration outside of the existing bony envelope is a major challenge in the field of orofacial surgery. In this study, we demonstrate that a highly porous, resorbable scaffold fabricated using additive manufacturing techniques enables reproducible extra-skeletal bone formation and prevents bone resorption. An additively manufactured medical grade polycaprolactone (mPCL) biphasic scaffold mimicking the architecture of the jaw bone, consisting of a 3D-printed outer shell overlying an inner highly porous melt electrowritten scaffold, was assessed for its ability to support dimensionally stable bone regeneration in an extraskeletal ovine calvarial model. To investigate bone formation capacity (stage 1), 7 different constructs placed under a protective dome were assessed 8 weeks post-implantation: Empty control, Biphasic scaffold with hydrogel (PCL-Gel), PCL-Gel with 75 or 150 μg of BMP-2 (PCL-BMP-75 and PCL-BMP-150), hydrogel only (Gel), Gel containing 75 or 150 μg of BMP-2 (Gel-BMP-75 and Gel-BMP-150). To assess dimensional stability (stage 2), in a separate cohort, 5 animals were similarly implanted with 2 samples of each of the Gel-BMP-150 and PCLBMP-150 groups, and after 8 weeks of healing, the protective domes were removed and titanium implants were placed in the regenerated bone and allowed to heal for a further 8 weeks. Bone formation and osseointegration were assessed using micro-computed tomography, histology and histomorphometry. In stage 1, enhanced bone formation was found in the BMP-2 containing groups, especially the PCL-BMP constructs whereby regeneration of full bone height was achieved in a reproducible manner. There was no significant bone volume increase with the higher dose of BMP-2. In the dimensional stability assessment (stage 2), after the rtemoval of the protective dome, the biphasic scaffold prevented bone resorption whereas in the absence of the scaffold, the bone previously formed in the hydrogel underwent extensive resorption. This was attributed to the space maintenance properties and dimensional stability of the biphasic scaffold. Titanium implants osseointegrated into the newly formed bone within the biphasic scaffolds. In conclusion, additively manufactured biphasic scaffolds functionalized with BMP-2 facilitated dimensionally stable bone regeneration that supported dental implant osseointegration

    Off-the-Shelf Tissue-Engineered Vascular Conduits: Clinical Translation

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