オドントグロッサムリングスポットウイルスCy-1株RNAのゲノム構成および韓国株との比較

The complete nucleotide sequince of the genomic RNA of odontoglossum ringspot virus Cy-1 strain(ORSV Cy-1) was determined using cloned cDNA. This sequence is 6611 nucleotides long containing four open reading frames, which correspond to 126 K,183 K,31 K and 18 K proteins. The 5' non-coding region of ORSV Cy-1 is 62 nucleotides. The ORFs encoded a 126 K polypeptide and a 183 K read-through product in which helicase-sequence and polymerase-sequence motifs are found. The5' non-coding region,which extends from bases 1 to 62 has 2G residues and the ribosome binding site (AUU). The3' non-coding region of ORSV Cy-1 composes 414 nucleotides in length. The genomic organization of ORSV Cy-1 is nearly identical to that of ORSV Korean strain(ORSV-K). However, the ORF encoding 183 K protein overlapes the ORF encoding 31 K protein in ORSV Cy-1, but not in ORSV-K. The 183 K read-through product of ORSV Cy-1 is 16 amino acids longer than that of ORSV-K. The homology of the nucleotide sequences of ORSV Cy-1 and ORSV-K is 96%.オドントグロッサムリングスポットウイルスCy-1株（ORSV Cy-1）のcDNAクローンを用いて完全長ゲノムRNAの塩基配列を決定した。この配列の長さは6611塩基であり、126K、183K、31K、および18Kのタンパク質に対応する4個のオープンリーディングフレーム（ORF）を含んでいた。ORSV Cy-1の5'非翻訳領域は62塩基であった。126Kおよび、その183Kリードスル―産物をコードするORF内にはヘリカーゼとポリメラーゼの塩基配列モチーフが含まれていた。5'非翻訳領域である1から62塩基目の領域には2個のG残基とリボソーム結合領域（AUU）を保持していた。ORSV Cy-1の3'非翻訳領域は414塩基長であった。ORSV Cy-1の遺伝子構成は、韓国株（ORSV-K）とほとんど同じであった。しかしながら、ORSV Cy-1の183Kタンパク質をコードするORFは31Kタンパク質をコードしているORFと一部重なっていたが、ORSV－Kでは重なっていなかった。ORSV Cy-1およびORSV-Kの183Kタンパク質のアミノ酸数を比較すると、ORSV Cy-1とORSV-Kの塩基配列の相同性は96％であった

Functional analysis of βC1 protein encoded by Tomato leaf curl Java virus, a monopartite begomovirus

Tomato leaf curl disease is among the most important limiting factors that affect tomato production in world wide including south East Asia. Recently, we have isolated tomato leaf curl Java virus, a whitefly-transmitted monopartite begomovirus, which belongs to the family Geminiviridae. ToLCJAV have been shown to be associated with a novel signal stranded DNA satellite, known as [beta]DNA. Post transcriptional gene silencing (PTGS) in plants is a natural defense response against virus infections, and as a counter defense strategy, viruses have evolved to encode silencing suppressor proteins. The present work we have identified the suppressor activities encoded by [beta]C1 protein encoded by ToLCJAV DNA[beta] satellite, which poses a serious threat to Indonesia

Identification of a new interaction mode between the Src homology 2 domain of C-terminal Src kinase (Csk) and Csk-binding protein/phosphoprotein associated with glycosphingolipid microdomains

This research was originally published in Journal of Biological Chemistry. Hiroaki Tanaka, Ken-ichi Akagi, Chitose Oneyama, Masakazu Tanaka, Yuichi Sasaki, Takashi Kanou, Young-Ho Lee, Daisuke Yokogawa, Marc-Werner Dobenecker, Atsushi Nakagawa, Masato Okada and Takahisa Ikegami. Identification of a new interaction mode between the Src homology 2 domain of C-terminal Src kinase (Csk) and Csk-binding protein/phosphoprotein associated with glycosphingolipid microdomains. Journal of Biological Chemistry. 2013; 288, 15240-15254. © the American Society for Biochemistry and Molecular Biology