16 research outputs found

    Anti-inflammatory activity of the sesquiterpene lactone diacethylpiptocarphol in dextransulfate sodium-induced colitis in mice

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    Ethnopharmacological relevance: Sesquiterpene lactones are organic compounds derived mainly from plants that exhibit anti-inflammatory and antitumor activities being one of the key mechanism of action of NF-kB pathway and synthesis of cytokines such as IL-1 and TNF-alpha. Aim of the study: The overall objective of the present study was to evaluate the anti-inflammatory action of a sesquiterpene lactone diacethylpiptocarphol (DPC) from Vernonia scorpioides (Lam.) Pers. and parthenolide (PTH) in Balb-c mice with DSS-induced colitis. Materials and methods: The anti-inflammatory effects of Intraperitonial administration of DPC (5 mg/kg/day) were evaluated in Balb/c mice with DSS-induced colitis, and further the body weight measurement, TNF-alpha and TGF-beta level was determined. Results: After intraperitoneal treatment for one week, DSS-induced colitis was significantly reduced in mice treated with either of both sesquiterpenes lactones, as witnessed by reduced cellular infiltration, tissue damage, TNF-alpha production, and enhanced production of TGF-beta. Conclusions: Sesquiterpene lactone DPC, isolated from Vernonia scorpioides showed anti-inflammatory activity, in this experimental model of colitis the sesquiterpene lactones DPC and PTH exhibit equal anti-inflammatory activity

    The value of universally available raw NMR data for transparency, reproducibility, and integrity in natural product research

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    Covering: up to 2018 With contributions from the global natural product (NP) research community, and continuing the Raw Data Initiative, this review collects a comprehensive demonstration of the immense scientific value of disseminating raw nuclear magnetic resonance (NMR) data, independently of, and in parallel with, classical publishing outlets. A comprehensive compilation of historic to present-day cases as well as contemporary and future applications show that addressing the urgent need for a repository of publicly accessible raw NMR data has the potential to transform natural products (NPs) and associated fields of chemical and biomedical research. The call for advancing open sharing mechanisms for raw data is intended to enhance the transparency of experimental protocols, augment the reproducibility of reported outcomes, including biological studies, become a regular component of responsible research, and thereby enrich the integrity of NP research and related fields

    Liquidation of Corporation

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    Import 22/07/2015Diplomov√° pr√°ce se zab√Ĺv√° procesem likvidace obchodn√≠ch spoleńćnost√≠ v ńĆesk√© republice. C√≠lem t√©to pr√°ce je popsat postup likvidace obchodn√≠ch spoleńćnost√≠ a upozornit na nńõkter√© probl√©my, kter√© proces likvidace s sebou pŇôin√°Ň°√≠, pouk√°zat na ŇôeŇ°en√≠ z√°kladn√≠ch organizańćn√≠ch a ekonomick√Ĺch ot√°zek souvisej√≠c√≠ s likvidac√≠ a propojit pr√°vn√≠ a daŇąov√Ĺ pohled na likvidaci. Pr√°ce je rozdńõlena do ńćtyŇô kapitol. V prvn√≠ kapitole jsou definov√°ny jednotliv√© pr√°vn√≠ formy obchodn√≠ch spoleńćnost√≠ a moŇĺn√© zpŇĮsoby jejich zruŇ°en√≠. Druh√° kapitola se zab√Ĺv√° popisem likvidańćn√≠ho procesu, kdy jsou pops√°ny jednotliv√© kroky likvid√°tora v prŇĮbńõhu likvidace. TŇôet√≠ kapitola je vńõnov√°na osobńõ likvid√°tora, podm√≠nky nutn√© k v√Ĺkonu likvid√°tora, povol√°n√≠ likvid√°tora do funkce. ńĆtvrt√° kapitola je vńõnov√°na praktick√Ĺm probl√©mŇĮm spojen√Ĺmi s procesem likvidace.Thesis deals with the process of liquidation of corporations in the Czech Republic. The aim of this thesis is to describe the procedure of liquidation and to point out some possible problems that could occur during liquidation. The legal and tax point view of the topic will also be included. Thesis is divided into 4 main chapters. Different legal forms of the corporations and the ways of dissolution of companies are defined in the first chapter. The second chapter pursues the process of liquidation. One part of this chapter is devoted to activities of the liquidator. The person of the liquidator, including his competences and required skills, is described in the third chapter. The last chapter is focused on practical problems concerning the process of liquidation.119 - Katedra pr√°vavelmi dobŇô

    Preliminary studies of alternative feed additives for broilers: Alternanthera brasiliana extract, propolis extract and linseed oil

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    The influence of alternative treatments using fluidextracts of Alternanthera brasiliana, propolis resin and linseed oil on the performance and blood biochemistry of broilers was evaluated. The study was done with five treatments: basal diet (negative control); basal diet + 40 ppm avylamicin and 120 ppm monensin (positive control); basal diet + A. brasiliana extract (180 mL/200 kg of feed); basal diet + propolis extract (200 mL/200 kg of feed) and basal diet + linseed oil (2.5% replacing soybean oil). Propolis and A. brasiliana extracts improved broiler performance from 14 to 21 days, whereas linseed oil had no effect. The findings of this experiment revealed that A. brasiliana and propolis extracts can be used as antimicrobials, but further studies are necessary to find the best concentration in broiler diets

    Sesquiterpene lactones from the leaves of Hedyosmum brasiliense (Chloranthaceae)

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    Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Hedyosmum brasiliense Miq. is an endemic aromatic arborescent shrub that is the only representative of the Chloranthaceae in Brazil. There have been few studies seeking to determine its chemical constituents and/or pharmacological effects. This work describes the isolation and identification of sesquiterpene lactones from the leaves, including guaianolides, elemanolides and a lindenanolide. These were tested against Mycobacterium tuberculosis, together with podoandin, onoseriolide and some other common phenolics. The structures of the isolated compounds were determined based on extensive analysis of 1D and 2D NMR spectroscopic and MS data, as well as comparison with published data. The compounds found were the guaianolides, 1,2-epoxy-10 alpha-hydroxy-podoandin and 1-hydroxy-10,15-methylenepodoandin, the elemenolide 15-acetoxy-isogermafurenolide and the lindenanolide 8 alpha/beta,9 alpha-hydroxy-onoseriolide, along with the previously isolated guaianolide podoandin, the lindenanolide onoseriolide and the elemenolide 15-hydroxy-isogermafurenolide. The phenolic compounds isolated were scopoletin, vanillin, vanillic acid, protocatechuic aldehyde and ethyl caffeate. The isolated sesquiterpene lactones did not show anti-mycobacterial activity against isoniazid-sensitive M. tuberculosis cultures at concentrations of 1-30 mu M. (C) 2012 Elsevier Ltd. All rights reserved.87126132Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)FINEPUFPRConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES

    Cytotoxic, Antitumour and Antimetastatic Activity of Two New Polyacetylenes Isolated from Vernonia scorpioides (Lam.) Pers

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    Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Vernonia scorpioides (Lam.) Pers., popularly known as Enxuga, Erva-de-SAo SimAo and Piraca, has been used in folk medicine for its anti-inflammatory, wound healing and antimicrobial properties. Two polyacetylenes, 5-octa-2,4,6-triynyl-furan-2(5H)-one (1) and 8-hydroxy 3-4 dihydrovernoniyne (2), were isolated from the dichloromethane extract fraction of V.scorpioides. In this study, polyacetylene 1 demonstrated a more potent cytotoxic activity than 2 in the tumour cell lines examined, and cytotoxicity was found to be comparable to a commercial drug (p>0.05) in melanoma cells. No significant cytotoxic effect was observed in normal cell lines. Furthermore, polyacetylene 1 induced an in vitro increase in caspase-3 activity in B16F10 cells. When polyacetylene 1 was administered intraperitoneally (i.p.) in mice, a reduction in solid tumour volume and metastasis was observed in mice injected with B16F10 cells. An increase in locomotor activity was also observed in mice with solid tumours, and an inhibition of mechanical hypersensitivity was observed in a mouse model of metastasis. Notably, no significant morphological change was observed in several organs harvested from the treated mice. In conclusion, in vitro and in vivo anticancer activity of polyacetylene 1 was consistently observed and involved the induction of apoptosis by the activation of caspase-3. The anticancer activity demonstrated by polyacetylene 1, together with the absence of preliminary toxicological effects, represents a new and interesting option for the management of neoplastic disease.1135307315Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Fundacao de Apoio a Pesquisa Cientofica e Tecnologica do Estado de Santa Catarina (FAPESC)Pro-Reitoria de Pesquisa, Pos-Graduacao, Extensao e Cultura of the Universidade do Vale do Itajai (ProP-PEC/UNIVALI), BrazilConselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES