Srs2 and RecQ homologs cooperate in mei-3-mediated homologous recombination repair of Neurospora crassa

Homologous recombination and post-replication repair facilitate restart of stalled or collapsed replication forks. The SRS2 gene of Saccharomyces cerevisiae encodes a 3′–5′ DNA helicase that functions both in homologous recombination repair and in post-replication repair. This study identifies and characterizes the SRS2 homolog in Neurospora crassa, which we call mus-50. A knockout mutant of N.crassa, mus-50, is sensitive to several DNA-damaging agents and genetic analyses indicate that it is epistatic with mei-3 (RAD51 homolog), mus-11 (RAD52 homolog), mus-48 (RAD55 homolog) and mus-49 (RAD57 homolog), suggesting a role for mus-50 in homologous recombination repair. However, epistasis evidence has presented that MUS50 does not participate in post-replication repair in N.crassa. Also, the N.crassa mus-25 (RAD54 homolog) mus-50 double mutant is viable, which is in contrast to the lethal phenotype of the equivalent rad54 srs2 mutant in S.cerevisiae. Tetrad analysis revealed that mus-50 in combination with mutations in two RecQ homologs, qde-3 and recQ2, is lethal, and this lethality is suppressed by mutation in mei-3, mus-11 or mus-25. Evidence is also presented for the two independent pathways for recovery from camptothecin-induced replication fork arrest: one pathway is dependent on QDE3 and MUS50 and the other pathway is dependent on MUS25 and RECQ2

STUDY ON APPLICATION OF DRAG-REDUCING TRIMETHYLOLETHANE SLURRY IN DISTRICR COOLING SYSTEM

Trimethylolethane (TME) trihydrate slurry is a proper phase-change material for latent heat transportation in cooling systems since it has excellence heat transfer performance and high thermal capacity. Additives, oleyl bishydroxyethyl methyl ammonium chloride (as surfactant) and sodium salicylate (as counter-ion), were used as drag-reducer substance. These additives not only induce drag reduction in TME slurry, but also control particle growth and agglomeration. In this study, energy saving estimation of drag-reducing TME slurry application in district cooling system is invesitgated. It is found that drag-reducing TME slurry gives remarkable pumping power suppresion in the district cooling system. To maintain high performance of heat exchangers, heat transfer enhancement technique may be needed in those exchangers

CATCHABILITY DIFFERENCE OF GILL NET AND COLLAPSIBLE BAITED POT FOR JAPANESE ROCK CRAB

The objective of this experiment is to know the catchabjlity of gill net and collapsible baited pot on Japanese rock crab. Result shows that gillnet caught more than twenty species other than Japanese rock crab

Redox Engineering of Myoglobin by Cofactor Substitution to Enhance Cyclopropanation Reactivity

Kagawa Y., Oohora K., Himiyama T., et al. Redox Engineering of Myoglobin by Cofactor Substitution to Enhance Cyclopropanation Reactivity. Angewandte Chemie - International Edition, (2024); https://doi.org/10.1002/anie.202403485.Design of metal cofactor ligands is essential for controlling the reactivity of metalloenzymes. We investigated a carbene transfer reaction catalyzed by myoglobins containing iron porphyrin cofactors with one and two trifluoromethyl groups at peripheral sites (FePorCF₃ and FePor(CF₃)₂, respectively), native heme and iron porphycene (FePc). These four myoglobins show a wide range of Fe(II)/Fe(III) redox potentials in the protein of +147 mV, +87 mV, +42 mV and −198 mV vs. NHE, respectively. Myoglobin reconstituted with FePor(CF₃)₂ has a more positive potential, which enhances the reactivity of a carbene intermediate with alkenes, and demonstrates superior cyclopropanation of inert alkenes, such as aliphatic and internal alkenes. In contrast, engineered myoglobin reconstituted with FePc has a more negative redox potential, which accelerates the formation of the intermediate, but has low reactivity for inert alkenes. Mechanistic studies indicate that myoglobin with FePor(CF₃)₂ generates an undetectable active intermediate with a radical character. In contrast, this reaction catalyzed by myoglobin with FePc includes a detectable iron–carbene species with electrophilic character. This finding highlights the importance of redox-focused design of the iron porphyrinoid cofactor in hemoproteins to tune the reactivity of the carbene transfer reaction

Structural health monitoring and damage detection using an intelligent parameter varying (IPV) technique

Most structural health monitoring and damage detection strategies utilize dynamic response information to identify the existence, location, and magnitude of damage. Traditional model-based techniques seek to identify parametric changes in a linear dynamic model, while non-model-based techniques focus on changes in the temporal and frequency characteristics of the system response. Because restoring forces in base-excited structures can exhibit highly non-linear characteristics, non-linear model-based approaches may be better suited for reliable health monitoring and damage detection. This paper presents the application of a novel intelligent parameter varying (IPV) modeling and system identification technique, developed by the authors, to detect damage in base-excited structures. This IPV technique overcomes specific limitations of traditional model-based and non-model-based approaches, as demonstrated through comparative simulations with wavelet analysis methods. These simulations confirm the effectiveness of the IPV technique, and show that performance is not compromised by the introduction of realistic structural non-linearities and ground excitation characteristics

Low-density lipoprotein receptors play an important role in the inhibition of prostate cancer cell proliferation by statins

AbstractBackgroundThere are some reports about the antitumor effects of statins in these days. Statins decrease the level of cholesterol in the blood by inhibiting 3-hydroxy-3-methylglutaryl-coenzyme A reductase. Inhibition of this enzyme decreases intracellular cholesterol synthesis. Thus, the expression of low-density lipoprotein receptor (LDLr) is increased to import more cholesterol from the bloodstream. In this study, we assessed the effects of statins on the proliferation of prostate cancer cells, and studied the relationship between the expression of LDLr and the effects of statins.MethodsSimvastatin was used in the experiments. We studied the effect of simvastatin on PC-3 and LNCaP cell proliferation using the MTS assay, and evaluated the expression of LDLr after administration of simvastatin by quantitative polymerase chain reaction and Western blotting. Intracellular cholesterol levels in the prostate cancer cells were measured after administration of simvastatin. Furthermore, small interfering RNA (siRNA) was used to knockdown the gene expression of LDLr.ResultsIn PC-3 cells, simvastatin inhibited cell proliferation. In LNCaP cells, only a high concentration of simvastatin (100μM) inhibited cell proliferation. In LNCaP cells, the protein level of LDLr was increased by simvastatin. In PC-3 cells, the protein levels of LDLr were unregulated. In PC-3 cells, but not in LNCaP cells, intracellular cholesterol levels were significantly decreased by simvastatin. After knocking down LDLr expression by siRNA, intracellular cholesterol levels were decreased, and cell proliferation was inhibited by simvastatin in LNCaP cells.ConclusionSimvastatin inhibited prostate cancer cell growth by decreasing cellular cholesterol and could be more effective in androgen-independent prostate cancer, where there is loss of regulation of LDLr expression. LDLr was shown to play an important role in the statin-induced inhibition of prostate cancer cell proliferation. These results suggest that future studies evaluating the cholesterol-lowering effects of statin may lead to new approaches to the prevention and treatment of prostate cancer

LPE法による高品質SOI層の形成技術に関する研究

University of Tokyo (東京大学