39 research outputs found
Ruolo della cellula endoteliale nel danno epatico dopo ischemia - riperfusione. Studio ultrastrutturale
13TH INTERNATIONAL CONGRESS ON NEUTRON CAPTURE THERAPY - A NEW OPTION AGAINST CANCER
Stato dell'arte della BNCT dal punto di visto della clinica e della ricerca in campo fisico, ingegneristico, radiobiologico, chimico ..
Thirteenth International Congress on Neutron Capture Therapy - Preface
The Thirteenth International Congress on Neutron Capture
Therapy (ICNCT) was held in Florence, Italy, from November 2–8,
2008. A selection of 98 out of the total of 222 papers, which were
presented at this meeting, is reported in this special issue of
Applied Radiation and Isotope
Morphologic and cytofluorimetric study of the rat coloncarcinoma DHDK12TRb cell line after boron neutron capture therapy
Thirteenth International Congress on Neutron Capture Therapy - BNCT: a new option against cancer
Efficacy of boron neutron capture therapy on liver metastases of colon adenocarcinoma: optical and ultrastructural study in the rat
The effect of neutron boron capture therapy (BNCT)
was studied in rat tumor liver cells after induction of the liver
metastases by splenic inoculation of cells from DHA/K12/TRb
line. Ten days following the treatment, the BPA was injected
into rats and therefore the animals were sacrificed, the liver
was exposed to neutron irradiation and processed. In some
experiments the liver was reimplanted (after irradiation) in
syngenic animals and studied 3 days later, following
sacrifice. Samples of tissue obtained from metastasised and
non-metastasised areas of the liver parenchyma, before and
after the neutron irradiation, were examined in light
microscopy and electron microscopy. The analysis pointed
out damages induced by the neutron treatment in single
tumor cells mostly localised in the synusoidal blood stream.
Debris and apoptotic cells were sometimes observed in the
neoplastic nodules before treatment, while the tumor cell death
(apoptosis) increased in the tumor cells following BNCT
treatment. An intense scavenger activity of Kupffer cells after
irradiation was accompanied by a strong acid phosphatase
reaction detectable in wide cytoplasmic areas. In the liver
parenchyma of reimplanted animals, the presence of large
collagen bundles spread among the hepatocytes was observed
at electron microscopy
Selective Uptake of P-Boronophenylalanine by Osteosarcoma Cellsfor Boron Neutron Capture Therapy
Selective uptake of p-boronophenylalanine by osteosarcoma cells for boron neutron capture therapy
Osteosarcoma is the most common non-hematologic primary cancer type that develops in bone. Current osteosarcoma treatments combine multiagent chemotherapy with extensive surgical resection, which in some cases makes necessary the amputation of the entire limb. Nevertheless its infiltrative growth leads to a high incidence of local and distant recurrences that reduce the percentage of cured patients to less than 60%. These poor data required to set up a new therapeutic approach aimed to restrict the surgical removal meanwhile performing a radical treatment. Boron neutron capture therapy (BNCT), a particular radiotherapy based on the nuclear capture and fission reactions by atoms of 10B, when irradiated with thermal neutrons, could be a valid alternative or integrative option in case of osteosarcoma management, thanks to its peculiarity in selectively destroying neoplastic cells without damaging normal tissues. Aim of the present work is to investigate the feasibility of employing BNCT to treat the limb osteosarcoma. Boronophenylalanine (BPA) is used to carry 10B inside the neoplastic cells. As a first step the endocellular BPA uptake is tested in vitro on the UMR-106 osteosarcoma cell line. The results show an adequate accumulation capability. For the in vivo experiments, an animal tumor model is developed in Sprague-Dawley rats by means of an intrafemoral injection of UMR-106 cells at the condyle site. The absolute amounts of boron loading and the tumor to normal tissue 10B ratio are evaluated 2 h after the i.v. administration of BPA. The boron uptake by the neoplastic tissue is almost twice the normal one. However, higher values of boron concentration in tumor are requested before upholding BNCT as a valid therapeutic option in the treatment of osteosarcoma