392 research outputs found

    Transcriptome Analysis of Host-Associated Differentiation in \u3cem\u3eBemisia tabaci\u3c/em\u3e (Hemiptera: Aleyrodidae)

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    Host-associated differentiation is one of the driving forces behind the diversification of phytophagous insects. In this study, host induced transcriptomic differences were investigated in the sweetpotato whitefly Bemisia tabaci, an invasive agricultural pest worldwide. Comparative transcriptomic analyses using coding sequence (CDS), 5′ and 3′ untranslated regions (UTR) showed that sequence divergences between the original host plant, cabbage, and the derived hosts, including cotton, cucumber and tomato, were 0.11–0.14%, 0.19–0.26%, and 0.15–0.21%, respectively. In comparison to the derived hosts, 418 female and 303 male transcripts, respectively, were up-regulated in the original cabbage strain. Among them, 17 transcripts were consistently up-regulated in both female and male whiteflies originated from the cabbage host. Specifically, two ESTs annotated as Cathepsin B or Cathepsin B-like genes were significantly up-regulated in the original cabbage strain, representing a transcriptomic response to the dietary challenges imposed by the host shifting. Results from our transcriptome analysis, in conjunction with previous reports documenting the minor changes in their reproductive capacity, insecticide susceptibility, symbiotic composition and feeding behavior, suggest that the impact of host-associated differentiation in whiteflies is limited. Furthermore, it is unlikely the major factor contributing to their rapid range expansion/invasiveness

    Exploring Valid Reference Genes for Quantitative Real-time PCR Analysis in \u3cem\u3ePlutella xylostella\u3c/em\u3e (Lepidoptera: Plutellidae)

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    Quantitative real-time PCR (qRT-PCR), a primary tool in gene expression analysis, requires an appropriate normalization strategy to control for variation among samples. The best option is to compare the mRNA level of a target gene with that of reference gene(s) whose expression level is stable across various experimental conditions. In this study, expression profiles of eight candidate reference genes from the diamondback moth, Plutella xylostella, were evaluated under diverse experimental conditions. RefFinder, a web-based analysis tool, integrates four major computational programs including geNorm, Normfinder, BestKeeper, and the comparative ΔCt method to comprehensively rank the tested candidate genes. Elongation factor 1 (EF1) was the most suited reference gene for the biotic factors (development stage, tissue, and strain). In contrast, although appropriate reference gene(s) do exist for several abiotic factors (temperature, photoperiod, insecticide, and mechanical injury), we were not able to identify a single universal reference gene. Nevertheless, a suite of candidate reference genes were specifically recommended for selected experimental conditions. Our finding is the first step toward establishing a standardized qRT-PCR analysis of this agriculturally important insect pest

    Detecting top-Higgs at high energy e+e−e^{+}e^{-} colliders

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    We calculate the contributions of the top-Higgs ht0h_{t}^{0} predicted by topcolor assisted technicolor(TC2) models to e+e−⟶t‾cν‾eνee^{+}e^{-}\longrightarrow \overline{t}c\overline{\nu}_{e}\nu_{e} and compare the results with the contributions of ht0h^{0}_{t} to the processes e+e−⟶Zht0⟶Zt‾ce^{+}e^{-}\longrightarrow Z h^{0}_{t}\longrightarrow Z\overline{t}c and e+e−⟶γht0⟶γt‾ce^+e^-\longrightarrow \gamma h^{0}_{t} \longrightarrow \gamma \overline{t}c. We find that e+e−⟶t‾cν‾eνee^{+}e^{-} \longrightarrow\overline{t}c\overline{\nu}_{e}\nu_{e} is very sensitive to ht0h^{0}_{t}, which can be easy detected via this process at high-energy e+e−e^{+}e^{-} collider(LC) experiments with s≥500\sqrt{s}\geq 500 GeVGeV, as long as its mass below the t‾t\overline{t}t threshold. The process e+e−⟶γt‾ce^{+}e^{-} \longrightarrow \gamma\overline{t}c also can be used to detect ht0h^{0}_{t} at LC experiments.Comment: latex file, 10 pages, 4 eps figure, submitted to Phys. Lett.

    Effects of Plant Virus and Its Insect Vector on Encarsia Formosa, a Biocontrol Agent of Whiteflies

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    In this study, we investigated the tritrophic interactions among a persistently transmitted plant virus, Tomato yellow leaf curl virus (TYLCV), its insect vector, the sweetpotato whitefly Bemisia tabaci, and a parasitoid, Encarsia formosa Gahan, one of the most extensively used biological control agents. As an emerging invasive pest worldwide, the two most damaging whiteflies are B. tabaci B and Q cryptic species. On healthy tomato plants, parasitoid-induced mortality was significantly higher in B. tabaci B than in Q. In contrast, similar mortality levels of B and Q were observed on TYLCV-infected plants. A higher rate of parasitism was consistently observed in B, independent of the TYLCV infection. Similarly, the life history traits of E. formosa were influenced by both TYLCV and the two cryptic species of B. tabaci. Specifically, E. formosa parasitizing B had a greater adult longevity and shorter developmental time on healthy plants, whereas the parasitoids developing from Q has a greater adult longevity on TYLCV-infected plants. The emergence rate of E. formosa was unaffected by either B. tabaci cryptic species or the virus. These results suggest that the vector-borne pathogen can manipulate the host suitability of a parasitoid and hence the parasitoid-host interactions
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