Новый способ выделения компонентов эфирного масла из плодов фенхеля обыкновенного

The aim of the work was to prove experimentally the possibility of extraction of main essential oil components from the Foe-niculum vulgare Mill. fruits by perfluoro organic solvents Novec 1230 and Novec 7100. Materials and methods. For study purposes, we used Foeniculum vulgare Mill. fruits with particle fraction of 0.1-0.5 mm. Quantitative and qualitative analyses of extracts were carried out by GC-MS and RP-HPLC methods. Two methods of extraction were used: simple maceration, plant raw material / solvent ratio 1:10 w/v, temperature 24±1 °С, maceration time 24 hours; circulation method: plant raw material / solvent ratio 1:5 w/v, solvent circulation time from 1 to 5 hours. Results and discussion. During maceration, the highest yield of anethole was observed in n-hexane, 96±4%, and in solvent Novec 7100, 94±4%, whereas in solvent Novec 1230, the yield of anethole was three times less, 28±2%. Solvents Novec 7100/1230 extracted identical compounds, and trans-anethole was the dominant one among them. The yield of anethole within two hours of solvent circulation was up to 82±4% for Novec 7100, and 89±4% for Novec 1230. Conclusions. The possibility of extraction of main components of essential oil from the plant raw material with perfluoro organic solvents Novec 1230/7100 by the example of Foeniculum vulgare Mill. fruits was experimentally tested. It was determined that the level of solvent molecule fluorination influenced its extraction properties with respect to anethole and fatty oil.Цель исследования - экспериментально проверить возможность экстракции основных компонентов эфирного масла из плодов фенхеля обыкновенного с помощью перфторорганических растворителей Novec 1230/7100. Показано, что при мацерации наибольший выход анетола наблюдался в н-гексан (96±4%) и в растворитель Novec 7100 (94±4%), при этом в растворитель Novec 1230 переходило в три раза меньше анетола (28±2%). В растворители Novec 1230/7100 переходят идентичные вещества, причем транс-анетол среди них доминирует. Выход анетола за два часа циркуляции растворителя достигал 82±4% (Novec 7100) и 89±4% (Novec 1230). Экспериментально проверена адекватность выдвигаемой рабочей гипотезы о возможности экстракции основных компонентов эфирного масла из эфиромасличного растительного сырья с помощью перфторорганических растворителей Novec 1230/7100 на примере плодов фенхеля обыкновенного. Обнаружено, что степень фторированности молекулы растворителя влияет на его экстракционные свойства в отношении анетола и жирного масла

Study of baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots

The aim of this study was to investigate the kinetics of baicalin hydrolysis in the process of its extraction from Scutellaria baicalensis Georgi roots. Materials and methods. For the studies, Scutellaria baicalensis Georgi roots with a particle range of 0.1.0.5 mm were used. The method of extraction was a simple maceration during a specified period of time, the ratio of plant raw material : extractant was 1:10 w/v at the temperature of 24±1°C. Baicalin and baicalein contents were analyzed by reverse phase high performance liquid chromatography (RP HPLC) at the analytical wavelength of 275 nm. The extractant was a water solution of ethanol 26, 43, 59, 72, 81, 97±1% v/v. The time of the extraction was from 1 to 24 hours. Results. The experimental points of dependency of baicalin concentration in the extract on the time of extraction for ethanol solutions with a concentration of 43 and 72% v/v are closely approximated by a linear equation in coordinates lnC=f(t). The value of determination coefficient is more than R2>0,99. Half lifetime for baicalin has been calculated: for ethanol with the concentration of 43% v/v it is 4.3±0.7 hours, and for ethanol with the concentration of 72% v/v it is 42.3±1.8 hours. Conclusion. Baicalin hydrolysis kinetics in the process of its extraction from Scutellaria baicalensis Georgi roots with 43 and 72% v/v ethanol concentration. has been studied. It has been established that the process of baicalin hydrolysis is well described by the first order kinetic equation. The constants of baicalin hydrolysis during its extraction from Scutelaria baicalensis roots with ethanol having different concentrations have been calculated. Recommendations on technology optimization for baicalin or baicalein extraction from Scutellaria baicalensis Georgi roots have been given. © 2019 Volgograd State Medical University, Pyatigorsk Medical and Pharmaceutical Institute. All rights reserved

Development of the technology for obtaining a thick extract from fruits of milk thistle with the stage of ultrasonic influence

This article presents the results of the development of technology for obtaining a thick extract of milk thistle fruits using ultrasonic treatment of plant material and extractant in the soaking stage. Materials and Methods: For the research, crushed fruits of milk thistle from “Biokor” Ltd, Penza, Russia, series 011216 were used, and the shelf life is 2 years. The particle size of the plant raw material is 0.5–1.0 mm. An aqueous solution of ethanol of 70% by volume was used as an extractant. Ultrasonic influence on the plant material and extractant were carried out under the following conditions: “Bandelin SONOPULS HD 3200” installation, ultrasonic frequency of 20 kHz, sound system of extracting system 5, 10, and 15 min, emitter power 280 W, and temperature 25°С. Results and its Discussion: It was found that with the ultrasonic treatment of raw materials and extractant in the soaking stage, the output of the phalavolignan complex almost doubles. To achieve the maximum concentration of flavonolignans in the extract, 5 min of ultrasound exposure to a mixture of raw material and extractant. Conclusions: The application of ultrasound at the stage of soaking plant material in the technology of obtaining a thick extract from the fruits of milk thistle is justified. It was shown that under the proposed conditions for extraction of flavonolignans from milk thistle, ultrasound promoted an increase in the yield of flavonolignans from plant raw materials and did not adversely affect these biologically active substances in the extract

Разработка галеновых препаратов с антимикробной активностью из травы зверобоя продырявленного

This article describes the development galenicals with antimicrobial activity from Hypericum perforatum L. herb. Based on the working hypotheses, we developed a mathematical model. Its adequacy was verified experimentally, and it was noted that the working hypotheses suggested were not disapproved. Carrying out microbiological studies, we found that the maximum level of ethanol-water extract’s antimicrobial activity was observed in the samples obtained with ethanol 72 and 97 % v/v. It was determined that the level of antimicrobial activity of ethanol-water extracts (1:5 w/v) with ethanol 72 and 97 % v/v was equal to that of reference drug «Chlorhexidine bigluconate water solution 0.05 %». It was found that using the filtration method of extraction for one hour, it is possible to obtain the liquid extract (1:1 w/v) with the concentration of dry residue that equals 13.0 ± 0.7 w/v, and in two hours, we can obtain the tincture (1:5 w/o) with the yield of main biologically active substances more than 90 %. The comprehensive studies conducted increase theoretical knowledge in the field of development of galenicals with antimicrobial activity from Hypericum perforatum L. herb.Описаны комплексные исследования в области разработки галеновых препаратов с антимикробной активностью из травы зверобоя продырявленного. Обнаружено, что максимальный уровень антимикробной активности спиртоводных извлечений наблюдается у тех образцов, которые были получены при использовании этанола с концентрацией 72 и 97 об.% Уровень антимикробной активности спиртоводных извлечений (1:5 м/о) не уступает уровню активности препарата сравнения «Хлоргексидин биглюконат водный раствор 0,05 %». С помощью метода фильтрационной экстракции в течение 1 ч можно получить жидкий экстракт (1:1 м/о) с концентрацией сухого остатка 13,0 ± 0,7 % м/о, а за 2 ч можно получить настойку (1:5 м/о) с выходом основных БАВ более 90 %

Comprehensive studies for development of galenicals with antimicrobial activity from hypericum perforatum L. Herb

This article describes the development galenicals with antimicrobial activity from Hypericum perforatum L. herb. Based on the working hypotheses, we developed a mathematical model. Its adequacy was verified experimentally, and it was noted that the working hypotheses suggested were not disapproved. Carrying out microbiological studies, we found that the maximum level of ethanol-water extract’s antimicrobial activity was observed in the samples obtained with ethanol 72 and 97 % v/v. It was determined that the level of antimicrobial activity of ethanol-water extracts (1:5 w/v) with ethanol 72 and 97 % v/v was equal to that of reference drug «Chlorhexidine bigluconate water solution 0.05 %». It was found that using the filtration method of extraction for one hour, it is possible to obtain the liquid extract (1:1 w/v) with the concentration of dry residue that equals 13.0 ± 0.7 w/v, and in two hours, we can obtain the tincture (1:5 w/o) with the yield of main biologically active substances more than 90 %. The comprehensive studies conducted increase theoretical knowledge in the field of development of galenicals with antimicrobial activity from Hypericum perforatum L. herb. © 2019, Folium Ltd. All rights reserved

Results of study dealing with antimicrobial activity of model gel mixtures for dental use with juniper essential oil

Background: In the last decade the incidence of periodontal disease has increased many times, and, according to data from WHO, it has become a global problem. In Russia 98.5% of those examined have periodontal disease. Currently, there is a shortage of locally produced medical remedies for the treatment of periodontal diseases. This situation creates a need for their pharmaceutical development. Aim: To study the antimicrobialactivity of model gel mixtures for dental use, with juniper essential oil as the final stage, in the development of a new soft dosage form for dental use. Materials and methods: Model samples of gel with apple pectin and gel with carbomer 934 were obtained. Antimicrobial activity was determined according to GPI.1.2.4.0010.18 “Determination of the antimicrobial activity of antibiotics by agar diffusion”. Results: All samples showed an acceptable degree of antimicrobial activity, however developed gel samples turned out to be priority in comparison with the market drug. Conclusion: It was established that the developed samples have moderate and high antibacterial activity in relation to the studied microorganism strains and a more pronounced biocidal effect than the reference drug – Metrogil-Dent gel. © 2020, Advanced Scientific Research. All rights reserved

Study of plants of genus stachys on the example of Betonicaofficinalisl. Within the scientific course “pharmaceutical remake”

The paper describes a retrospective analysis of the use of common betony in traditional and modern medicine. The Stachys plants have not been used in modern domestic medicine yet. Therefore, based on the historical experience of the use of common betony in traditional medicine, we have emphasized its value as a plant with high therapeutic potential. In this study, we used the so-called “Pharmaceutical remake”, intended to revive interest in the now-forgotten objects of flora, which were previously widely used in medicine. The polyphenol structure of betony grass was studies with the use of the reverse-phase and graduate elution high-performance liquid chromatography. The presence of glycosides of diosmin, acacetin, luteolin and apigenin was established. The grass also contains hydroxycinnamic acids such as chlorogenic and rosemary acids, with the latter dominating in the polyphenol complex. The composition of plant terpenes in a hexane extraction has been studied by gas-liquid chromatography with mass spectrometric detection. It was determined that the terpenoid composition of betony is mainly represented by sesquiterpens: aromadendrene and germacrene, and monoterpenes: 3-carene and dihydrocarveol. © 2016, International Journal of Pharmacy and Technology. All rights reserved

At the threshold of technological solutions: Analysis of russian market of medicines used for the treatment and prevention of periodontal diseases

Background: Epidemiological study data indicate that inflammatory periodontal diseases take the 2nd place after caries among people under 30 years of age, and among people over 30 years of age they take the 1st place reaching 85-90%. The aim of the study: To study the existing market of medicine remedies used for the treatment and prevention of periodontal diseases, for further objective selection of the composition, as well as development of the technology of a new targeted dosage form (DF). Materials and methods: In this article materials of different available information resources and methods of marketing research are used. Results: The analysis of medicines used for the treatment and prevention of periodontal diseases (PD) was carried out. It was noted that the main method of prevention and treatment is still the use of antimicrobial agents. Conclusion: Thus, the development of original domestic medicines for PD treatment is an urgent task of pharmaceutical science. The use of natural components associated with nutrients in their composition becomes profitable from the standpoint of rational pharmacotherapy and marketing events. In this regard, the long-term technological and analytical experience accumulated in our team, which is associated with the scientific field “Pharmaceutical remake”, allows us to consider ethereal oils, in particular, the essential oil of galberries Juniperus communis L. as the target objects of research. © 2020, Advanced Scientific Research. All rights reserved

ИЗУЧЕНИЕ ПРОЦЕССА ЭКСТРАКЦИИ ЛИПОФИЛЬНЫХ ВЕЩЕСТВ ИЗ ПЛОДОВ РАСТОРОПШИ ПЯТНИСТОЙ С ПОМОЩЬЮ ФРЕОНА R141B

The aim of the article is to experimentally study the extraction of sum lipophilic substances from the Silybum marianum L. fruits by refrigerant R141b. Materials and methods. For the studies we used milled plant raw material “Silybum marianum L. fruits” and “Silybum marianum L. fruits meal” with particle range 0.1-0.5 mm, and refrigerant R141b. For the extraction was used filtration method of extraction. For quantitative determination of sum lipophilic substances was used gravimetric method of analysis. Results. It was found that at temperature 25 °C, at filtration velocity of solvent through the plant raw material layer 0.96 l/(kgh) and at extract volume / raw material mass ratio (2:1), not less than 98 % of lipophilic substances was extracted by the refrigerant R141b from the Silybum marianum L. fruits during 2.4 h. Conclusions. Extraction dynamic of the sum of lipophilic substances from the Silybum marianum L. fruits by filtration method with refrigerant R141b at different volume velocity of its feed has been studied. As a result of the studies it was found that the yield of the sum of lipophilic substances achieved not less 98 %, during the 2.4 h, at the filtration velocity of extractant 0.96 l/(kg • h) and at extract volume / raw material mass ratio (2:1). It was suggested that the filtration method of extraction can be used for the development of a complex technology treatment from the Silybum mari-anum L. fruits.Цель работы - экспериментально изучить экстракцию суммы липофильных веществ из плодов расторопши пятнистой с помощью фреона R141b. Материалы и методы. В исследованиях использовали измельченное растительное сырье с фракцией частиц 0,1 -0,5 мм и фреон R141b. Для экстракции использовали фильтрационный метод экстракции. Для количественного определения суммы липофильных веществ использовали гравиметрический метод анализа. Результаты. Найдено, что при температуре 25°С, скорости фильтрации растворителя через слой сырья 0,96 л/(кгч) и соотношении объем извлечения / масса сырья (2:1), за 2,4 часа, фреон R141b из плодов расторопши извлекает > 98 % липофильных веществ. Выводы. Изучена динамика извлечения суммы липофильных веществ из плодов расторопши пятнистой с применением фильтрационного метода экстракции с помощью фреона R141b в качестве экстрагента при различной объемной скорости его подачи. В результате проведенных исследований найдено, что выход суммы липофильных веществ > 98 % в течение 2,4 часов при скорости фильтрации - 0,96 л/(кгч), и при соотношении объем экстракта/масса ЛРС 2:1 (о/м). Показано, что фильтрационный способ экстракции липофильных веществ может быть развит в отдельную технологическую операцию в составе комплексной технологии по переработке плодов расторопши пятнистой