Species Identification of Marine Fishes in China with DNA Barcoding

DNA barcoding is a molecular method that uses a short standardized DNA sequence as a species identification tool. In this study, the standard 652 base-pair region of the mitochondrial cytochrome oxidase subunit I gene (COI) was sequenced in marine fish specimens captured in China. The average genetic distance was 50-fold higher between species than within species, as Kimura two parameter (K2P) genetic distances averaged 15.742% among congeners and only 0.319% for intraspecific individuals. There are no overlaps of pairwise genetic variations between conspecific and interspecific comparisons apart from the genera Pampus in which the introgressive hybridization was detected. High efficiency of species identification was demonstrated in the present study by DNA barcoding. Due to the incidence of cryptic species, an assumed threshold is suggested to expedite discovering of new species and biodiversity, especially involving biotas of few studies

The Forecasting Model of Bicycle Parking Demand on Campus Teaching and Office District

AbstractIn order to forecast the demand of the campus teaching and office district bicycle parking generation rate more accurate with the introduction of multi-model, which regarding affects are not carried on more considerations with the other parking demand influencing factor according to the actual investigation and study data. The paper analyzes the three types of regional architecture characteristics of bicycle parking demand, and gives the parking demand - supply forecast model which based on the parking generation rate model. The model considers the trip distance, parking distance, the facility cycling rate and the use factor which are also integrated in the model, thus obtains the number of bicycle parking facility which should construct actually. Finally the paper proposes a calculation example to expound the practical application of this model