Phenolic compounds and biological activity of Capsicum annuum L.

The objective of this study was to evaluate antifungal and antioxidant activities of vegetable extracts (Capsicum annuum L. cv. Dora, cv. Strizanka, cv. Morava), grown in Serbia. Different experimental models have included the determination content of total phenolics, total flavonoids, antioxidant capacity and minimum inhibitory concentration of the extract. The phenolic composition of different extracts was determined by the high-performance liquid chromatography (HPLC) method. The highest amounts of phenols and highest antioxidant capacity were found in the cultivar Dora extracts. The highest phenolic content has been shown in cultivar Strizanka ethanol extract. All of the extracts showed strong antimicrobial activity. On the basis of the results obtained, the extracts were found to serve as a potential source of natural antioxidants and antimicrobial due to their marked activity. The obtained results may be useful in the evaluation of new dietary and food products.Key words: High-performance liquid chromatography (HPLC), phenolic components, antimicrobial activity, antioxidant activity

Stanje, mogućnosti i perspektive oplemenjivačkih progarma krastavaca (Cucumis Sativus L.) u Srbiji

Climatic conditions provide growing cucumbers Serbia as an open field and in greenhouses. Growing in greenhouses makes it possible to fruits of cucumber from domestic production on the market for almost the entire year. This capability dramatically increases the economic efficiency and thereby directly encouraged producers to cultivation of this plant species. Markets directly affected the characteristics of the fruit cucumbers that will be desired. This trend breeding companies must follow in order to offer farmers varieties that corresponds to preferences market. Also, farmers want high yield. The yield of cucumber is directly dependent on the expression of sex type plants.Klimatski uslovi Srbije omogućavaju gajenje krastavaca kako na otvorenom polju, tako i u zaštićenom prostoru. Gajenje u zaštićenom prostoru omogućava da se plodovi krastavca iz domaće proizvodnje mogu naći na tržištu skoro tokom celo godine. Ova mogućnost drastično povećava njegovu ekonomsku profitabilnost a time direktno podstiče prozvođače na gajenje ove biljne vrste. Tržišta direktno utiču na karakteristike krastavaca koje će bit cenjene. Ovaj trend oplemenjivačke kompanije morju pratiti kako bi proizvođačima ponudile sortiment koji odgovara željama kupaca. Takođe, proizvođači zahtevaju i visok prinos. Prinos kod krastavca direktno zavisi od tipa polne ekspresije biljaka

Microfilaremic dirofilaria repens infection in patient from Serbia

We report a case of Dirofilaria repens infection causing microfilaremia in a patient from Serbia. Serum samples tested positive for D. repens IgG by ELISA. Our findings and those of others suggest the parasite's progressive adaptation to humans. Clinicians should be aware that microfilaremia can develop during Dirofilaria spp. infections

Izbor genotipova paradajza na tolerantnost prema suši

Screening collections aimed to make the selection of genotypes tolerant to drought in the vegetative stage of intensive growth of plants, which would start the program selection to obtain recombinant genotypes according to the abiotic factor. The criteria for screening were the diversity of genotypes for the number of sheets of the first flower branches and number of flower branches into the optimal mode of irrigation regime and reduced by 40 % . On the basis of the analysis genotypes: the number of leaves to the first floral branch G 106, 114, 121 and 122 ( 0 % ), and genotype G102, 114, and 125 for the characteristic number of lateral branches.Paradajz je široko prilagođen različitim podnebljima gajenja, međutim, njegov rast i razviće je prilično osetljiv na različite uslove spoljne sredine, uključujući salinitet, sušu, vlagu, ekstremne temperature, mineralne toksičnosti, kao i zagađenje životne sredine. Postoji ograničenje genetske varijacije za abiotičku toleranciju na stres u okviru kultivisanih vrsta i većina komercijalnih sorti se smatraju umereno do veoma osetljive na različite vrste stresa. Ispitivanje je izvršeno na 11 genotipa paradajza poreklom iz populacije domaćih i odomaćenih genotipova prikupljenih iz Srbije, a pripadaju kolekciji paradajza Instituta za povrtarstvo u Smederevskoj Palanci. Skrining kolekcije imao je za cilj da se izvrši izbor genotipova tolerantnih na sušu u vegetativnoj fazi intenzivnog porasta biljaka, čime bi se započeo program selekcije na dobijanje rekombinovanih genotipova prema ovom abiotskom faktoru. Kriterijumi za skrining bili su divergentnost genotipova za broj listova do prve cvetne grane i broj cvetnih grana u: optimalnom režimu navodnjavanja i redukovanom režimu za 40%. Na osnovu izvedenih analiza izdvojeni su genotipovi: za broj listova do prve cvetne grane G 106, 114, 121 i 122 (0%), kao i genotipovi G102, 114, i 125 za osobinu broja bočnih grana koji će predstavljati bazu za dobijanje rekombinovanih genotipova i početak selekcije na otpornost na sušu

Increased il-33 and il-17 in colorectal carcinoma patients with severe disease

© 2020, University of Kragujevac, Faculty of Science. All rights reserved. Colorectal cancer (CRC) represents one of the most common cancers. It is frequently diagnosed at advanced stages, indicating on need for new diagnostic markers. The aim of this study was to determine systemic and fecal values of IL-17 and IL-33 in patients with CRC and the relationship with clinicopathological aspects of disease. The blood samples and feces liquid fraction of 50 patients with CRC were analyzed. Serum and fecal levels of IL-33 and IL-17 were measured using sensitive enzyme-linked immu-nosorbent assay (ELISA) kits. Fecal levels of Il-33 and IL-17 were increased in CRC patients with poor tumor tissue differentiation. Serum IL-33 and fecal IL-17 were increased in patients with presence of lung/liver metastasis or peritoneal carcinomatosis, respec-tively, while enhanced fecal IL-33 was detected only in patients with peritoneal carcinomatosis. Positive correlation between IL-33 and IL-17 values in sera and feces, respectively was also observed. We believe that increased local values of IL-33 and IL-17, reflected trough higher fecal concentration, in CRC patients with poor tumor tissue differentiation and with presence of lung/liver metastasis or peritoneal carcinomatosis may be considered as a sign of the tumor’s malignant progression and, consequently, of a poor prognosis for patients

Fractional flow reserve of intermediate lesions on collateral donor coronary arteries after myocardial infarction

Fractional flow reserve (FFR) is the gold standard for the functional assessment of coronary arteries. The aim of this study was to evaluate the relation between angiography, QCA and FFR in borderline lesions on collateral donor coronary arteries. In addition, FFR is compared with the angiographic appearance of collaterals to infarction-related arteries and echocardiographically assessed viability of infarct related the LV wall. In 60 patients with previous IM and occluded IRA, functional assessment of borderline coronary stenosis (30-70% DS) on collaterals donor artery was performed. We have not found statistically significant differences in these parameters between groups with different angiographic appearances of collaterals and different viability of distal myocardium. However, we found higher FFR values in diabetic patients (p=0.018). Higher FFR values in diabetic patients reveal the negative effects of diabetes on collateral growth and myocardial viability

Karakteristike proizvodnje semena povrća u Srbiji

Seed production in the Republic of Serbia is a very important sector of agriculture. This production is taking place in public and private sector. Although seed production in Serbia can meet domestic demands, a certain amount of seed is being imported. Unfortunately, some vegetable producers (except for organic, dynamic or bio production) use a certain amount of vegetable seeds from their own mercantile production. Crops produced from such seed generate low yields due to poor seed quality (varietal impurity, low germination) and health. The study covered four years (2007-2010) and the surfaces under the vegetable crops aimed for seed production: 2007 (956 ha of seed produced in Serbia of which 64.9 ha produced by the Institute for Vegetable crops); 2008 (1152 in Serbia, 137 ha the Institute); 2009 (1219 ha in Serbia, 242.1 ha the Institute); 2010 (976 ha in Serbia, 198.7 the Institute).Proizvodnja semena je veoma bitna sa aspekta održivosti poljoprivrede jedne zemlje. U današnjoj eri sekvencioniranja, mapiranja i nadasve zaštite istraženih sekvenci, ovakve tendencije predstavljaju mogućnost za objektivnu zavisnost od istraživača (vlasnika) koji su odredili pojedine sekvence odgovorne za nasleđivanje pojedinih - traženih osobina novoselekcionisanih sorata. Posebno poglavlje ove problematike predstavljaju i GMO organizmi i njihova prezentacija širokoj proizvodnoj praksi kroz novonastale selekcije. U Republici Srbiji nije dozvoljena upotreba GMO selekcija ali smo svesni da se vrši pritisak na izmenu pozitivnih propisa i dozvolu širenja upotrebe ovih organizama. Semenarstvo Republike Srbije je veoma značajna grana poljoprivredne proizvodnje kojom se u državu slivaju velike količine novca od prodaje semena domaćih selekcija. Povrtarstvo je samo jedan mali segment ove proizvodnje, ali veoma bitan, kako za domaće proizvođače –farmere koji se sve podložniji svetskim trendovima nastupa na organizovanim tržištima a koji zahtevaju određene karakteristike gajenih sorti, tako i za konzumente povrća. Ovi zahtevi savremenog tržišta najčešće su u suprotnosti sa kvalitetom. Današnje semenarstvo Srbije se odvija u državnom i privatnom sektoru. Iako proizvodnja semena može podmiriti domaće potrebe, izvesna količina semena se nabavlja uvozom. Ovaj uvoz je postojao i u vreme zatvorenog tržišta SFRJ, te su smenske kompanije navikle na tržišnu utakmicu. Problem, a iskazala ga je i Evropska Unija svojim zakonima, jeste da jedan deo povrtara, osim za organsku (dinamičku, bio i dr proizvodnju), koriste određene količine semena povrća iz sopstvene merkantilne proizvodnje. Ovakvi usevi ostvaruju niske prinose kako zbog kvaliteta semena (sortna čistoća, klijavost), tako i zbog zdravstvene ispravnosti semena Sortiment povrća koji nastaje u Republici Srbiji stvaran je u agroekološkim uslovima Balkanskog poluostrva te je zato i našlo je tržište širom Balkana, a u poslednje vreme, i Evrope. Cilj ovog rada je da se iskaže učešće u proizvodnji pojedinih povrtarskih vrsta izraženo po površinama i u količinama Instituta za povrtarstvo d.o.o u semenarskoj proizvodnji Srbije. Istraživani ciklus je obuhvatao 4 godine i u njemu je proizvedeno seme povrća na 956 ha 2007 (64.9 ha), 1152 (137) 2008. godine, 1219 ha (242.1ha) 2009. godine i 976 ha (198.7 ha) 2010. godine

Rapd fingerprinting of indigenous lysinibacillus fusiformis isolates from stabilized sludge and oil-polluted soil

The Lysinibacillus group are motile Gram-positive bacilli, with oval or spherical spores, oxidase and catalase positive, not grouped in strict chains, with strictly oxidative metabolism and very similar to Bacillus sphericus group. Two indigenous isolates of Lysinibacillus fusiformis from oil-polluted soil near gas station and stabilized sludge from petrochemical plant in Serbia (FOV – HIP “Petrohemija”, Pancevo) were tested on IAR and HMT and showed high sensitivity to neomycin, cephalexin and bacitracin, and were resistant to trimethoprim. Both isolates were tolerant to 100μg/ml of Zn and Co, 10μg/ml of Mo, but they differed in tolerance to 20μg/ml of Cd and 10μg/ml of Hg. The isolate from stabilized sludge showed moderate emulsification ability of xylol (E24 65.8) and mineral oil (E24 75.6). The isolate from oil-polluted soil showed very strong emulsification ability of xylol (E24 87.2) and moderate of mineral oil (E24 72.7). RAPD fingerprinting showed clear differences between the two Lysinibacillus fusiformis isolates

Morfološke i hemijske analize novih linija pastrnka (Pastinaca sativa L.) Instituta za povrtarstvo

In Serbia, a parsnip is often grown vegetable gardens and the gardens, grown for thickened roots that is used in spice who spravljanu delicious dishes. This paper presents the results of 12 new genotypes breeding - line parsnip Institute of Vegetable Crops Palanka. Their morphological and chemical characteristics were compared with the standard variety Panonski smooth. In the three-year period from 2009 - of 2012 were analyzed by the following morphological characteristics: the average root weight, the average weight of leaf, root collar diameter , the diameter of the root environment and the emergence of secondary roots. Is determined by the chemical analysis of the dry matter , the total minerals , total sugars , and water. The aim was to examine the quality of new lines parsnip than the standard variety Panonski smooth and select the new lines for the recognition of new varieties of parsnip Institute of Vegetable Crops.Pastrnak (Pastinaca sativa L.) pripada familiji Apiaceae, po svojim prehrambenim i lekovitim svojstvima spada u red najvrednijih povrtarskih kultura. U Srbiji pastrnak je često gajeno povrće po okućnicama i baštama,gaji se radi zadebljalog korena koji se koristi ko začin pri spravljanu ukusnih jela. Koristan je za zdravlje jer poboljšava apetit, pospešuje rad bubrega,deluje na smanjenje krvnog pritiska. U radu su prikazani rezultati ispitivanja 12 novo selekconisanih genotipova- linija pastrnka Instituta za povrtarstvo Palanka. Njihove morfološke i hemijske osobine upoređene su sa standardnom sortom Panonski glatki. U trogodišnjem periodu od 2009 – 2012.godine analizirane su sledeće morfološke osobine: prosečna masa korena, prosečna masa lista, prečnik vrata korena, prečnik sredine korena i pojava sekundarnih korenova. Hemijskom analizom određena je suva materija, ukupni minerali, ukupni šećeri i voda. Cilj je bio da se ispita kvalitet novo stvorenih linija pastrnka u odnosu na standarnu sortu Panonski glatki i odaberu nove linije za priznavanje novih sorti pastrnka Instituta za Povrtarstvo