28 research outputs found
Increased serum IL-6 level time-dependently regulates hyperalgesia and spinal mu opioid receptor expression during CFA-induced arthritis
Interleukin (IL)-6 is known to cause pro- and anti-inflammatory effects during different stages of inflammation. Recent therapeutic investigations have focused on treatment of various inflammatory
disorders with anti-cytokine substances. As a result, the aim of this study was to
further elucidate the influence of IL-6 in hyperalgesia and edema during different stages of Complete Freund’s Adjuvant (CFA)-induced arthritis (AA) in male Wistar rats. AA was induced by a single subcutaneous injection of CFA into the rats’ hindpaw. Anti-IL-6 was administered either daily or weekly during the 21 days of study. Spinal mu opioid receptor (mOR) expression was detected by Western blotting. Daily and weekly treatment with an anti-IL-6 antibody significantly decreased paw edema in the AA group compared to the AA control group. Additionally, daily and weekly anti-IL-6 administration significantly reduced
hyperalgesia on day 7 in the AA group compared to the AA control group; however, there were significant increases in hyperalgesia in the antibody-treated group on days 14 and 21 compared to the AA control group. IL-6 antibody-induced increases in hyperalgesia on the 14th and 21st days after CFA injection correlated with a time-dependent, significant reduction
in spinal mOR expression during anti-IL-6 treatment. Our study confirmed the important
time-dependent relationship between serum IL-6 levels and hyperalgesia during AA. These
results suggest that the stages of inflammation in AA must be considered for anti-hyperalgesic and anti-inflammatory interventions via anti-IL-6 antibody treatment
Neutrino Spectroscopy of the Early Phase of Nearby Supernovae
Neutrinos emitted during stellar core collapse up to their trapping phase
carry information about the stage from which the Supernova explosion process
initiates. The dominant emission mechanism is by electron capture on
free protons and f-p shell nuclei and the spectrum of these neutrinos is a
function of the ambient physical conditions within the core as well as the
nuclear equation of state. The number of collapse phase which can be
detected by Super-Kamioka and Sudbury Neutrino Observatory from a Supernova
within 1 kpc, and their generic energy spectra are given.Comment: 9 pages of text and tables plus 2 pages of figures. Accepted for
publication in Phys. Rev. Lett. on 11th Jul., 1997. Please e-mail Comments
etc. to [email protected]
Massive stars as thermonuclear reactors and their explosions following core collapse
Nuclear reactions transform atomic nuclei inside stars. This is the process
of stellar nucleosynthesis. The basic concepts of determining nuclear reaction
rates inside stars are reviewed. How stars manage to burn their fuel so slowly
most of the time are also considered. Stellar thermonuclear reactions involving
protons in hydrostatic burning are discussed first. Then I discuss triple alpha
reactions in the helium burning stage. Carbon and oxygen survive in red giant
stars because of the nuclear structure of oxygen and neon. Further nuclear
burning of carbon, neon, oxygen and silicon in quiescent conditions are
discussed next. In the subsequent core-collapse phase, neutronization due to
electron capture from the top of the Fermi sea in a degenerate core takes
place. The expected signal of neutrinos from a nearby supernova is calculated.
The supernova often explodes inside a dense circumstellar medium, which is
established due to the progenitor star losing its outermost envelope in a
stellar wind or mass transfer in a binary system. The nature of the
circumstellar medium and the ejecta of the supernova and their dynamics are
revealed by observations in the optical, IR, radio, and X-ray bands, and I
discuss some of these observations and their interpretations.Comment: To be published in " Principles and Perspectives in Cosmochemistry"
Lecture Notes on Kodai School on Synthesis of Elements in Stars; ed. by Aruna
Goswami & Eswar Reddy, Springer Verlag, 2009. Contains 21 figure
Effect of heating and cooling combination therapy on patients with chronic low back pain: study protocol for a randomized controlled trial
Role of serum interleukin-6 level on hyperalgesia and spinal mu-opioid receptor expression during the complete Freund's adjuvant-induced chronic inflammation
Background: Interleukin (IL-6) is known to cause pro- and anti-inflammatory effects during different stages of inflammation. The purpose of this study was to elucidate the effects of IL-6 on hyperalgesia, edema and the changes in the spinal mu opioid receptor expression during different stages of complete Freund’s adjuvant (CFA)-induced arthritis (AA) in rats.
Materials and Methods: In this study, AA was induced by a single subcutaneous injection of CFA into rats’ hindpaw. The rats with arthritis were divided into four groups, each consisted of three subgroups (n= 6). Anti-IL-6 was administered either daily or weekly during the 21-day study period. Spinal mu-opioid receptor (mOR) expression was detected by Western blotting.
Results: Daily treatment with an anti-IL-6 antibody significantly decreased the paw edema in the AA group compared to the control one (P=0.001), but daily and weekly anti-IL-6 administrations significantly increased the hyperalgesia in the antibody-treated group on the 14th and 21st days post-treatment (P=0.001, P=0.01, respectively). The administration of IL-6 antibody not only increased hyperalgesia in a time-dependent manner, but also caused a significant reduction in the spinal mOR expression on the 14th and 21st days post-CFA injection (P=0.01, P=0.001, respectively).
Conclusion: Results can indicate the importance of a time-dependent relationship between the serum IL-6 level and hyperalgesia during the AA. Moreover, the results suggest that the stages of inflammation in AA must be considered for anti-hyperalgesic and anti-inflammatory interventions via anti-IL-6 antibody treatment
Effect of methanol extract of Althaea root on contractile function of rat's tracheal smooth muscle
Background: The sudden involuntary muscular contractions of the air ways is a very common disease in human societies. Study on medical plant extracts to affect contractile activity of the tracheal smooth muscle can be useful manner in control of pulmonary obstructive sickness. Objectives: This research was designed to determine the effect of Althaeas' root methanol extract on contractile function of rat tracheal smooth muscle. Methods: In this experimental study, 36 tracheas of a healthy and male of 18 Sprague Dawley rats were dissected in 6 groups. The effect of Althaeas' root methanol extract concentrations (0.2, 0.6, 2.6, 6.6, 14.6, 140.6 μg/ml) and epinephrine (5 μm) in presence and absence of 1μM propranolol under one gram tension based on isometric method was assayed. This assay was recorded in an organ bath containing kerebs-hancelit solution on tracheal smooth muscle contractions by KCl (60 mM) induction. Results: Epinephrine (5 μm) alone and root methanol extract concentrations (0.6-140.6 μg/ml) reduced the tracheal smooth muscle contractions induced by KCl (60 mM) dose-dependently. Propranolol couldn't reduce the antispasmodic effect of root methanol extract concentrations, but it could reduce the antispasmodic effect of Epinephrine on tracheal smooth muscle contractions. Conclusion: Althaeas' root methanol extract inhibited the tracheal smooth muscle of rat contractions dose dependently, but it seems that β-Adrenergic receptors are not engage in this process. Realizing the mechanism of this process can be useful in treatment of pulmonary obstructive disease