Extraction and Characterization of Peppermint (Mentha piperita) Essential Oil and its Assessment as Antioxidant and Antibacterial

ABSTRACT       The aim of this study is to extract and characterize peppermint essential oil and assess it as antioxidant and antibacterial activity. The extraction processes, chemical composition, total phenolic contents (TPC), total flavonoid contents (TFC), antioxidant and antibacterial activity were studied. The oil was extracted by three methods: steam distillation, solvent and soxhelet.  The results indicated that the highest oil extraction was obtained by soxhelet method 1.5±0.12 and 1.2±0.12 ml/100 gm and the lowest result recorded by steam distillation 1.1±0.09 and 0.9±0.14 ml/100gm from fresh and dry samples, respectively. The chemical composition of fresh and dry sample were determined by using gas chromatography and the results showed that there were 26 components of volatile compounds were identified in the essential oil isolated from peppermint. The antibacterial potential of mint essential oils was evaluated by Muller Hinton agar well diffusion method against selected bacteria. The essential oils showed higher activity against Staphylococcus aureus and Salmonella 19±1.41 and 16.5±2.12 mm, respectively, for fresh sample and showed lower activity against Escherichia coli and Klebsilla pneumonia 8.5±0.70 mm and nil, respectively, for dry sample. The TPC of fresh and dry samples were 14.00±0.12 and 8.80±0.09 mg /kg, respectively. TFC of the mint essential oil was determined in comparison with rutin were 8.1±0.09 and 5.0±0.07mg/kg, for fresh and dry sample, respectively. The results showed decrease in peroxide value at 30, 60, 90, 120 and 150 min compared with blank sample without essential oil for wet and dry mint. DPPH inhibition percentages were recorded and inhibition concentrations at 50% activity (IC50) were 0.651±0.09, 0.683±0.6 and 0.161±0.07 mg/mL for wet, dry samples and standard respectively. It can be recommended that further study on sensory analysis of food products containing mint essential oil to evaluate its acceptability and shelf life.

Capability of Trichoderma viride to Produce Cellulolytic and Pectolytic Enzymes

Background: Species of the genus Trichoderma have been used in the food and textile industries to produce cellulases and other enzymes that degrade complex polysaccharide structures. Trichoderma species have been utilized to make cellulases and other enzymes that break down intricate polysaccharide structures in the food and textile industries. The study investigated the nutritional requirements and the production of enzymes by the fungus Trichoderma viride fungus' ability to produce enzymes).Methods: We used a medium supplemented with cellulolytic and pectic substances for enzyme production.Results: The results of the study proved that. Methionine, Glutamic acid, and leucine effectively enhanced mycelial growth. Findings regarding impact of pH level on the development of the fungus T. viride indicated that the maximum growth was at pH 5.0. However, growth decreased dramatically with increasing pH values. We examined the power of the fungus T. viride to produce cellulolytic and pectolytic enzymes in various substrates in the current study.Conclusion: The findings demonstrated that Pectin was the best substrate for pectolytic enzyme synthesis, whereas Carboxymethyl cellulose (CMC) was the best substrate for the cellulolytic enzyme

Quality Evaluation of Honey Obtained from Different Sources

Abstract Honey is a food rich in nutrients essential for hu man life such as sugars, proteins, vitamins and minerals and uses honey as food and medicine. Honey is considered one of the most foods which can be adulterated, therefore this study aimed to assess the quality of different samples of natural and industrial honey and matching the results with the international standards. Chemical and physical methods were used in the study to determine the various chemical and physicochemical characteristics. For determinat ion of sugars, a High Performance Liquid Chro matography (HPLC) was used. The results indicate that there were no significant differences in most of the chemical and physicochemical characteristics of natural honey and industrial honey. The results also indicated that the various honey types contained sucrose (10.7%-3.48%), fructose (14.74% -39.01%), glucose (14.09% -35. 6%). Ho wever, the industrial honey was the richest type in nutrients, so its use in industry is highly reco mmended

Effects of hospital facilities on patient outcomes after cancer surgery: an international, prospective, observational study

Background Early death after cancer surgery is higher in low-income and middle-income countries (LMICs) compared with in high-income countries, yet the impact of facility characteristics on early postoperative outcomes is unknown. The aim of this study was to examine the association between hospital infrastructure, resource availability, and processes on early outcomes after cancer surgery worldwide.Methods A multimethods analysis was performed as part of the GlobalSurg 3 study-a multicentre, international, prospective cohort study of patients who had surgery for breast, colorectal, or gastric cancer. The primary outcomes were 30-day mortality and 30-day major complication rates. Potentially beneficial hospital facilities were identified by variable selection to select those associated with 30-day mortality. Adjusted outcomes were determined using generalised estimating equations to account for patient characteristics and country-income group, with population stratification by hospital.Findings Between April 1, 2018, and April 23, 2019, facility-level data were collected for 9685 patients across 238 hospitals in 66 countries (91 hospitals in 20 high-income countries; 57 hospitals in 19 upper-middle-income countries; and 90 hospitals in 27 low-income to lower-middle-income countries). The availability of five hospital facilities was inversely associated with mortality: ultrasound, CT scanner, critical care unit, opioid analgesia, and oncologist. After adjustment for case-mix and country income group, hospitals with three or fewer of these facilities (62 hospitals, 1294 patients) had higher mortality compared with those with four or five (adjusted odds ratio [OR] 3.85 [95% CI 2.58-5.75]; p<0.0001), with excess mortality predominantly explained by a limited capacity to rescue following the development of major complications (63.0% vs 82.7%; OR 0.35 [0.23-0.53]; p<0.0001). Across LMICs, improvements in hospital facilities would prevent one to three deaths for every 100 patients undergoing surgery for cancer.Interpretation Hospitals with higher levels of infrastructure and resources have better outcomes after cancer surgery, independent of country income. Without urgent strengthening of hospital infrastructure and resources, the reductions in cancer-associated mortality associated with improved access will not be realised

Impact of opioid-free analgesia on pain severity and patient satisfaction after discharge from surgery: multispecialty, prospective cohort study in 25 countries

Background: Balancing opioid stewardship and the need for adequate analgesia following discharge after surgery is challenging. This study aimed to compare the outcomes for patients discharged with opioid versus opioid-free analgesia after common surgical procedures.Methods: This international, multicentre, prospective cohort study collected data from patients undergoing common acute and elective general surgical, urological, gynaecological, and orthopaedic procedures. The primary outcomes were patient-reported time in severe pain measured on a numerical analogue scale from 0 to 100% and patient-reported satisfaction with pain relief during the first week following discharge. Data were collected by in-hospital chart review and patient telephone interview 1 week after discharge.Results: The study recruited 4273 patients from 144 centres in 25 countries; 1311 patients (30.7%) were prescribed opioid analgesia at discharge. Patients reported being in severe pain for 10 (i.q.r. 1-30)% of the first week after discharge and rated satisfaction with analgesia as 90 (i.q.r. 80-100) of 100. After adjustment for confounders, opioid analgesia on discharge was independently associated with increased pain severity (risk ratio 1.52, 95% c.i. 1.31 to 1.76; P < 0.001) and re-presentation to healthcare providers owing to side-effects of medication (OR 2.38, 95% c.i. 1.36 to 4.17; P = 0.004), but not with satisfaction with analgesia (beta coefficient 0.92, 95% c.i. -1.52 to 3.36; P = 0.468) compared with opioid-free analgesia. Although opioid prescribing varied greatly between high-income and low- and middle-income countries, patient-reported outcomes did not.Conclusion: Opioid analgesia prescription on surgical discharge is associated with a higher risk of re-presentation owing to side-effects of medication and increased patient-reported pain, but not with changes in patient-reported satisfaction. Opioid-free discharge analgesia should be adopted routinely

Reducing the environmental impact of surgery on a global scale: systematic review and co-prioritization with healthcare workers in 132 countries

Abstract Background Healthcare cannot achieve net-zero carbon without addressing operating theatres. The aim of this study was to prioritize feasible interventions to reduce the environmental impact of operating theatres. Methods This study adopted a four-phase Delphi consensus co-prioritization methodology. In phase 1, a systematic review of published interventions and global consultation of perioperative healthcare professionals were used to longlist interventions. In phase 2, iterative thematic analysis consolidated comparable interventions into a shortlist. In phase 3, the shortlist was co-prioritized based on patient and clinician views on acceptability, feasibility, and safety. In phase 4, ranked lists of interventions were presented by their relevance to high-income countries and low–middle-income countries. Results In phase 1, 43 interventions were identified, which had low uptake in practice according to 3042 professionals globally. In phase 2, a shortlist of 15 intervention domains was generated. In phase 3, interventions were deemed acceptable for more than 90 per cent of patients except for reducing general anaesthesia (84 per cent) and re-sterilization of ‘single-use’ consumables (86 per cent). In phase 4, the top three shortlisted interventions for high-income countries were: introducing recycling; reducing use of anaesthetic gases; and appropriate clinical waste processing. In phase 4, the top three shortlisted interventions for low–middle-income countries were: introducing reusable surgical devices; reducing use of consumables; and reducing the use of general anaesthesia. Conclusion This is a step toward environmentally sustainable operating environments with actionable interventions applicable to both high– and low–middle–income countries

International Journal of Multidisciplinary and Current Research Effect of solar drying using a natural convective solar drier on bacterial load and chemical composition of bayad (Bagrus bayad) fish flakes

Abstract Solar drying experiments of bayad (Bagrus bayad) fish flakes using a natural convective solar drier were conducted a

International Journal of Multidisciplinary and Current Research Effect of solar drying using a natural convective solar drier on bacterial load and chemical composition of bayad (Bagrus bayad) fish flakes

Abstract Solar drying experiments of bayad (Bagrus bayad) fish flakes using a natural convective solar drier were conducted a

Global variation in postoperative mortality and complications after cancer surgery: a multicentre, prospective cohort study in 82 countries

Background: 80% of individuals with cancer will require a surgical procedure, yet little comparative data exist on early outcomes in low-income and middle-income countries (LMICs). We compared postoperative outcomes in breast, colorectal, and gastric cancer surgery in hospitals worldwide, focusing on the effect of disease stage and complications on postoperative mortality. Methods: This was a multicentre, international prospective cohort study of consecutive adult patients undergoing surgery for primary breast, colorectal, or gastric cancer requiring a skin incision done under general or neuraxial anaesthesia. The primary outcome was death or major complication within 30 days of surgery. Multilevel logistic regression determined relationships within three-level nested models of patients within hospitals and countries. Hospital-level infrastructure effects were explored with three-way mediation analyses. This study was registered with ClinicalTrials.gov, NCT03471494. Findings: Between April 1, 2018, and Jan 31, 2019, we enrolled 15 958 patients from 428 hospitals in 82 countries (high income 9106 patients, 31 countries; upper-middle income 2721 patients, 23 countries; or lower-middle income 4131 patients, 28 countries). Patients in LMICs presented with more advanced disease compared with patients in high-income countries. 30-day mortality was higher for gastric cancer in low-income or lower-middle-income countries (adjusted odds ratio 3·72, 95% CI 1·70–8·16) and for colorectal cancer in low-income or lower-middle-income countries (4·59, 2·39–8·80) and upper-middle-income countries (2·06, 1·11–3·83). No difference in 30-day mortality was seen in breast cancer. The proportion of patients who died after a major complication was greatest in low-income or lower-middle-income countries (6·15, 3·26–11·59) and upper-middle-income countries (3·89, 2·08–7·29). Postoperative death after complications was partly explained by patient factors (60%) and partly by hospital or country (40%). The absence of consistently available postoperative care facilities was associated with seven to 10 more deaths per 100 major complications in LMICs. Cancer stage alone explained little of the early variation in mortality or postoperative complications. Interpretation: Higher levels of mortality after cancer surgery in LMICs was not fully explained by later presentation of disease. The capacity to rescue patients from surgical complications is a tangible opportunity for meaningful intervention. Early death after cancer surgery might be reduced by policies focusing on strengthening perioperative care systems to detect and intervene in common complications. Funding: National Institute for Health Research Global Health Research Unit