Research on the Influence of Music

As for question 1, based on the directed relationship between influencers and followers, we building a network of musicians based on influential relationships. A Music Influence Evaluation Model (MIEM) was also established, and the model formula is shown in the text. We then select the top 200 artists in the “music influence” ranking to build a subnet. The larger the subnet node, the more lines are extended. Indicating that the node represents the musician’s influence is large and extensive. From the graph, we can see that Bob Dylan is influential, but the breadth of influence is not enough; Miles Davis influenced a wide range of music factions.As for question 2, we have developed a Music Similarity Evaluation Model (MSEM) to calculate the contribution parameters of fifteen different music metrics. Using fully connected neural networks combined with triple loss to solve the answer. According to the characteristics of Triple Loss, we can make the similar nodes in the space closer together and the dissimilar nodes further apart. After training, our neural network is able to distinguish artists very well. The results were obtained: artists within genres are far more similar than artists between genres, and a classification image of musicians from different genres was produced.As for question 3, a comparative plot of characteristics revealed that music genres also have their own particular musical characteristics. The comprehensive analysis concludes that the difference between genres is mainly reflected by the six features of valence, tempo, mode, key, acousticness, and instrumentalness, and this result is verified by k-means clustering. By plotting the percentage of influence as well as the change of musical characteristics, it was concluded that the influence of genres changes over time; some musical characteristics in genres also change over time. Finally, the similarity between each faction is calculated and plotted as a heat map, and the genres with high similarity must have interrelated relationships with each other.As for question 4, we have developed a Music Influence T-test Model (MITM). We hypothesized that “influencers” would not influence followers to create music, and a t-test using SPSS rejected the original hypothesis and concluded that “influencers” would influence followers to create music. Additionally, Contagious Evaluation Model(CEM) was also be created. We established the “contagious” index and calculated the Pearson correlation coefficients between “contagious” and 15 musical characteristics, and obtained the results: energy, loudness, and acousticness are more “contagious” than other characteristics. Results: energy, loudness and acousticness are more “contagious” than other features.As for question 5, a time series plot of the variation for each musical characteristic with year was plotted and the analysis yielded the following conclusion: There are characteristics that signify revolutions in musical evolution from these data. For example, the music after 1960s showed changes characterized by higher rhythmicity, faster tempo, and fewer spoken words. Based on these musical evolutionary changes, combined with the “musical influence” we calculated earlier, we select five musical change-makers: The Beatles, Bob Dylan, The Rolling Stones, Miles Davis and Jimi Hendrix.As for question 6, we combined musical influences to identify the most influential musicians in each genre in each era as dynamic influencers to represent the music of the genre in that period. Creating images of their musical characteristics over time and analyzing them in relation to the history of musical development led to the conclusion that an artist’s musical identity changes with technology, social development, and changes in genre representation?As for question 7, a Network Connectivity Evaluation Model(NCEM) was developed to measure which artists in the music network were heavily influenced by external factors during the time period. The first and middle of the 20th century were found to be highly connected online, and this period coincided with a period of social upheaval, with the Cold War, World War II, the Industrial Revolution, and the rapid development of the Internet having a great impact on music, from which many new musical styles were born

The History and Outlook of Animal Drugs Treating Asthma, Chronic Bronchitis, and Haze Episode-induced Respiratory Diseases

Animal drugs have been historically applied in Chinese remedies for more than two thousands. It was reported that Chinese medical animals consisting of 1,590 species took up 12.5% of the total number of all TCM resources. Those animal drugs such as, earthworm, gecko, periostracum cicadae, and scorpios, of commonly used in China, are very remarkable and traditional for the treatment of asthma or chronic bronchitis. This review presents research advance of animal drugs possessing significant implications for the development of novel anti-asthma or chronic bronchitis drugs. The experimental studies and clinical efficacies against asthma and chronic bronchitis of animal drugs were summarized herein. Moreover, the potential utilization of animal drugs on inhibiting haze/fog induced respiratory diseases was also discusse

Ethyl methanesulfonate mutant library construction in Neopyropia yezoensis to provide germplasm resources for next-generation genome-selection breeding

With the development of the laver industry, germplasm depression has become a serious issue, and current cultivars cannot adapt to different aquaculture regions. In order to increasing the genetic diversity and developing more germplasm sources, it is urgent and reasonable to construct a mutant library with more new germplasms. In this research, a mutant library was constructed by ethyl methanesulfonate (EMS)-mutagenized archeospores, and the most optimal treatment procedure was determined by performing different mutagen concentrations (2.25%) and treatment times (30 min). A total of 1860 haploid thalli were produced as the M1 mutant population and further cultured into conchocelis clones for the reservation of germplasm resources. Among these, 667 individual thalli were evaluated for their phenotypic traits, including thallus length, thallus width, length/width, thallus shape, photosynthesis ability, thallus color, thallus margin, and specific growth speed. The mutation frequency of the length/width ratio was 17.39%, Fv/Fm and NPQ were 21.84% and 29.35%, respectively, and SGR was 13.59%. The mutation frequency of thallus color was 0.91%. This work may not only provide a basic practical reference guide for EMS-based mutant library construction for other seaweeds but, more importantly, also serve as a valuable resource for functional genomics research and laver breeding

Comparison of DNA histograms by standard flow cytometry and image cytometry on sections in Barrett's adenocarcinoma

<p>Abstract</p> <p>Background</p> <p>The purpose of this study was to compare DNA histograms obtained by standard flow cytometry (FC) and high fidelity image cytometry on sections (ICS) in normal gastrointestinal mucosa and Barrett's adenocarcinoma (BAC).</p> <p>Methods</p> <p>Archival formalin-fixed paraffin-embedded tissue blocks of 10 normal controls from 10 subjects and 42 BAC tissues from 17 patients were examined. DNA FC was performed using standard techniques and ICS was carried out by Automated Cellular Imaging System (ACIS). DNA ploidy histograms were classified into diploid with peak DNA index (DI) at 0.9–1.1, and aneuploid with peak DI > 1.1. DI values of aneuploid peaks were determined. Additionally, for DNA ICS, heterogeneity index (HI) representing DNA content heterogeneity, and histograms containing cells with DI > G2 were also identified.</p> <p>Results</p> <p>All control samples were diploid by both FC and ICS analyses. In BAC, FC showed diploid peaks in 29%, diploid peaks with additional aneuploid or tetraploid peaks in 57%, and 14% of the samples, respectively. In contrast, ICS showed aneuploid peaks in all the cases with peak DI > 1.25; 37 cases had peak DI between 1.25 and 2.25; and 5 cases had peak DI > 2.25. HI values (mean ± SD) were 11.3 ± 1.1 in controls and 32.4 ± 8.5 in BAC (p < 0.05). Controls had no G2 exceeding cells. However, 19/37 (51%) of the cases with primary peak DI < 2.25 had cells exceeding 9N.</p> <p>Conclusion</p> <p>ICS detects DNA aneuploidy in all BAC samples while FC missed the diagnosis of aneuploidy in 29%. In addition, ICS provides more information on HI and G2 exceeding rates.</p

DNA index determination with Automated Cellular Imaging System (ACIS) in Barrett's esophagus: Comparison with CAS 200

BACKGROUND: For solid tumors, image cytometry has been shown to be more sensitive for diagnosing DNA content abnormalities (aneuploidy) than flow cytometry. Image cytometry has often been performed using the semi-automated CAS 200 system. Recently, an Automated Cellular Imaging System (ACIS) was introduced to determine DNA content (DNA index), but it has not been validated. METHODS: Using the CAS 200 system and ACIS, we compared the DNA index (DI) obtained from the same archived formalin-fixed and paraffin embedded tissue samples from Barrett's esophagus related lesions, including samples with specialized intestinal metaplasia without dysplasia, low-grade dysplasia, high-grade dysplasia and adenocarcinoma. RESULTS: Although there was a very good correlation between the DI values determined by ACIS and CAS 200, the former was 25% more sensitive in detecting aneuploidy. ACIS yielded a mean DI value 18% higher than that obtained by CAS 200 (p < 0.001; paired t test). In addition, the average time required to perform a DNA ploidy analysis was shorter with the ACIS (30–40 min) than with the CAS 200 (40–70 min). Results obtained by ACIS gave excellent inter-and intra-observer variability (coefficient of correlation >0.9 for both, p < 0.0001). CONCLUSION: Compared with the CAS 200, the ACIS is a more sensitive and less time consuming technique for determining DNA ploidy. Results obtained by ACIS are also highly reproducible

Effect of narrow groove MAG welding oscillation parameters on weld formation

In order to solve the problem of narrow groove wall fusion, improving the weld formation, the welding test of different oscillation width and frequency is carried out by adopting the narrow high-speed swing MAG welding torch. On the basis of measuring the macro metallographic to determine the weld cross section size, the curve fitting of the variation trend of the weld section size with the swing width and the swing frequency is carried out. The effect of the high speed swing arc narrow groove welding MAG parameters on weld formation is studied. The results show that increasing the swing width or frequency can reduce the weld penetration and increase lateral wall penetration; when the swinging width makes side spacing greater than -05 mm and less than 1 mm, good weld forming will be gained; when the oscillation frequency increases to more than 5 Hz, weld finger penetration can be eliminated, but when it is above 9 Hz, increasing the swing frequency cannot cause obvious change of the dimension of weld. The effects of these oscillating parameters on weld formation can provide guidance for the selection of welding parameters