Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by -4

Em carried by (pSEC:huIP-10). The diagram shows the nisin-inducible promoter PnisA, the ribosome binding-site of (RBS), the usp45 signal peptide of the gene (SPusp), and the coding region for the mature moiety of IP-10. The open circle represents a rho-independent transcription terminator fused just downstream to the IP-10 gene (not to scale).<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p

Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by -3

Motaxis. T lymphocytes obtained directly from human peripheral blood were stimulated with human IL-2 for 12 days. Chemotaxis assays using Boyden chambers were made with supernatants sterilized by filtration of both recombinant and wild-type . To determine the number of chemoattracted cells, the membranes were stained with hematoxylin and the cells counted by using light microscopy at 1000 × (A). Cells in the lower chamber were incubated with specific anti-CD3+ and counted by FACS analysis (B). Supernatants of wild-type (WT) and PBS were used as negative controls and Zymosan-activated serum (S+) as a positive control.<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p

Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by -2

Of nisin for 1 hour. Culture A was in the presence of nisin all the time; culture B washed and suspended in fresh medium without inducer at hour 1 and 3, and culture C was washed and suspended in fresh medium without inducer only at hour 1. All cultures were grown a total of 6 hours. Protein extracts were analyzed by Western blot at hour 1, 3, and 6. The band in culture B at hour 6 (asterisk) represents the IP-10 "de novo" specifically secreted from hour 3 to 6 in the total absence of nisin demonstrating the ability of to keep expressing and producing IP-10 for at least three hours.<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p

Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by -0

Em carried by (pSEC:huIP-10). The diagram shows the nisin-inducible promoter PnisA, the ribosome binding-site of (RBS), the usp45 signal peptide of the gene (SPusp), and the coding region for the mature moiety of IP-10. The open circle represents a rho-independent transcription terminator fused just downstream to the IP-10 gene (not to scale).<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p

Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by -1

were prepared from cell-free samples and analyzed by Western blotting using anti-IP-10. Mature IP-10 was detected in all the induced (+) cultures in the 10 kDa range as expected. No signal was found for noninduced cultures (-) of recombinant NZpSEC:huIP-10. No immature or incomplete forms of IP-10 were detected. M, protein molecular marker.<p><b>Copyright information:</b></p><p>Taken from "Secretion of biologically active interferon-gamma inducible protein-10 (IP-10) by "</p><p>http://www.microbialcellfactories.com/content/7/1/22</p><p>Microbial Cell Factories 2008;7():22-22.</p><p>Published online 28 Jul 2008</p><p>PMCID:PMC2503953.</p><p></p