Additional file 4 of Immuno-digital invasive cleavage assay for analyzing Alzheimer’s amyloid ß-bound extracellular vesicles

Additional file 4: Supplemental Fig. 4. (A) Size distribution of APP Tg serum-derived EVs analyzed by a nanoparticle analyzer, qNano. (B) Representative image of APP Tg serum-derived EVs by electron microscopy. (C) Western blot analysis of Aß, ganglioside GM1, CD9, and ßIII tubulin in APP Tg serum-derived EVs. (D) Representative images of hippocampal sections immunostained with Aβ. Scale bars, 200 μm. (E) The levels of Aβ1-40 and Aβ1-42 in whole sera and serum-derived EVs of 12-month-old APP Tg mice were measured by conventional ELISA (n = 4 each). n.d., not detected