Design of mechanical metamaterials using a level-set based topology optimization method

Metamaterials are a family of artificially engineered materials consisting of an array of periodically arranged microstructures, offering unusual material properties that may not be easily found in nature. This paper will propose a new topological shape optimization method for the design of mechanical metamaterials with negative Poisson’s ratios, by integrating the numerical homogenization method with a powerful level set method. The homogenization method is used to calculate the effective properties of the microstructure, and the level set method is utilized to implement shape and topology optimization of the microstructure until the desired material properties are obtained. The proposed method can retain the unique features of the level set methods, while avoid unfavourable numerical issues occurred in the conventional level set methods. Several typical numerical examples are used to showcase the effectiveness of the proposed design method

Validation of the Cardiosphere Method to Culture Cardiac Progenitor Cells from Myocardial Tissue

At least four laboratories have shown that endogenous cardiac progenitor cells (CPCs) can be grown directly from adult heart tissue in primary culture, as cardiospheres or their progeny (cardiosphere-derived cells, CDCs). Indeed, CDCs are already being tested in a clinical trial for cardiac regeneration. Nevertheless, the validity of the cardiosphere strategy to generate CPCs has been called into question by reports based on variant methods. In those reports, cardiospheres are argued to be cardiomyogenic only because of retained cardiomyocytes, and stem cell activity has been proposed to reflect hematological contamination. We use a variety of approaches (including genetic lineage tracing) to show that neither artifact is applicable to cardiospheres and CDCs grown using established methods, and we further document the stem cell characteristics (namely, clonogenicity and multilineage potential) of CDCs.CPCs were expanded from human endomyocardial biopsies (n = 160), adult bi-transgenic MerCreMer-Z/EG mice (n = 6), adult C57BL/6 mice (n = 18), adult GFP(+) C57BL/6 transgenic mice (n = 3), Yucatan mini pigs (n = 67), adult SCID beige mice (n = 8), and adult Wistar-Kyoto rats (n = 80). Cellular yield was enhanced by collagenase digestion and process standardization; yield was reduced in altered media and in specific animal strains. Heparinization/retrograde organ perfusion did not alter the ability to generate outgrowth from myocardial sample. The initial outgrowth from myocardial samples was enriched for sub-populations of CPCs (c-Kit(+)), endothelial cells (CD31(+), CD34(+)), and mesenchymal cells (CD90(+)). Lineage tracing using MerCreMer-Z/EG transgenic mice revealed that the presence of cardiomyocytes in the cellular outgrowth is not required for the generation of CPCs. Rat CDCs are shown to be clonogenic, and cloned CDCs exhibit spontaneous multineage potential.This study demonstrates that direct culture and expansion of CPCs from myocardial tissue is simple, straightforward, and reproducible when appropriate techniques are used

IP packet forwarding based on comb extraction scheme

In this paper, we present an efficient IP packet forwarding technique and its architecture. One forwarding table is decomposed into two balanced smaller sub-forwarding tables by a novel splitting rule. Therefore, an IP lookup can be converted into a pair of small sub-lookups. The output of an incoming packet can be determined by comparing the information, attached to the matching sub-pre