Asteroseismic analysis of solar-mass subgiants KIC 6442183 and KIC 11137075 observed by Kepler

Asteroseismology provides a powerful way to constrain stellar parameters. Solar-like oscillations have been observed on subgiant stars with the \emph{Kepler\/} mission. The continuous and high-precision time series enables us to carry out a detailed asteroseismic study for these stars. We carry out data processing of two subgiants of spectral type G: KIC 6442183 and KIC 11137075 observed with the \emph{Kepler} mission, and perform seismic analysis for the two evolved stars. We estimate the values of global asteroseismic parameters: $\Delta\nu=64.9\pm 0.2$ $\mu$Hz and $\nu_{\rm max}=1225 \pm 17$ $\mu$Hz for KIC 6442183, $\Delta\nu=65.5\pm 0.2$ $\mu$Hz and $\nu_{\rm max}=1171 \pm 8$ $\mu$Hz for KIC 11137075, respectively. In addition, we extract the individual mode frequencies of the two stars. We compare stellar models and observations, including mode frequencies and mode inertias. The mode inertias of mixed modes, which are sensitive to the stellar interior, are used to constrain stellar models. We define a quantity $d\nu_{\rm m-p}$ that measures the difference between the mixed modes and the expected pure pressure modes, which is related to the inertia ratio of mixed modes to radial modes. Asteroseismic together with spectroscopic constraints provide the estimations of the stellar parameters: $M = 1.04_{-0.04}^{+0.01} M_{\odot}$, $R = 1.66_{-0.02}^{+0.01} R_{\odot}$ and $t=8.65_{-0.06}^{+1.12}$ Gyr for KIC 6442183, and $M = 1.00_{-0.01}^{+0.01} M_{\odot}$, $R = 1.63_{-0.01}^{+0.01} R_{\odot}$ and $t=10.36_{-0.20}^{+0.01}$ Gyr for KIC 11137075. Either mode inertias or $d\nu_{\rm m-p}$ could be used to constrain stellar models.Comment: 9 pages, 8 figures, 5 tables A&A accepte

The Al-induced proteomes of epidermal and outer cortical cells in root apex of cherry tomato \u27LA 2710\u27

This paper reports a laser capture microdissection-tandem mass tag-quantitative proteomics analysis of Al-sensitive cells in root tips. Cherry tomato (Solanum lycopersicum var. cerasiforme ‘LA2710’) seedlings were treated under 15 μM Al3+ activity for 13 d. Root-tip longitudinal fresh frozen tissue sections of 10 μm thickness were prepared. The Al-sensitive root zone and cells were determined using histochemical analysis of root-tips and micro-sections. A procedure for collecting the Al-sensitive cells using laser capture microdissection-protein extraction-tandem mass tag-proteomics analysis was developed. Proteomics analysis of 18 μg protein/sample with three biological replicates per treatment condition identified 3879 quantifiable proteins each associated with two or more unique peptides. Quantified proteins constituted a broad range of Kyoto Encyclopedia of Genes and Genomes pathways when searched in the annotated tomato genome. Differentially expressed proteins between the Al-treated and non-Al treated control conditions were identified, including 128 Al-up-regulated and 32 Al-down-regulated proteins. Analysis of functional pathways and protein-protein interaction networks showed that the Al-down-regulated proteins are involved in transcription and translation, and the Al-up-regulated proteins are associated with antioxidant and detoxification and protein quality control processes. The proteomics data are available via ProteomeXchange with identifier PXD010459 under project title ‘LCM-quantitative proteomics analysis of Al-sensitive tomato root cells’. Significance This paper presents an efficient laser capture microdissection-tandem mass tag-quantitative proteomics analysis platform for the analysis of Al sensitive root cells. The analytical procedure has a broad application for proteomics analysis of spatially separated cells from complex tissues. This study has provided a comprehensive proteomics dataset expressed in the epidermal and outer-cortical cells at root-tip transition zone of Al-treated tomato seedlings. The proteomes from the Al-sensitive root cells are valuable resources for understanding and improving Al tolerance in plants

Overexpression of Pear (Pyrus pyrifolia) CAD2 in Tomato Affects Lignin Content

PpCAD2 was originally isolated from the ‘Wangkumbae’ pear (Pyrus pyrifolia Nakai), and it encodes for cinnamyl alcohol dehydrogenase (CAD), which is a key enzyme in the lignin biosynthesis pathway. In order to verify the function of PpCAD2, transgenic tomato (Solanum lycopersicum) ‘Micro-Tom’ plants were generated using over-expression constructs via the agrobacterium-mediated transformation method. The results showed that the PpCAD2 over-expression transgenic tomato plant had a strong growth vigor. Furthermore, these PpCAD2 over-expression transgenic tomato plants contained a higher lignin content and CAD enzymatic activity in the stem, leaf and fruit pericarp tissues, and formed a greater number of vessel elements in the stem and leaf vein, compared to wild type tomato plants. This study clearly indicated that overexpressing PpCAD2 increased the lignin deposition of transgenic tomato plants, and thus validated the function of PpCAD2 in lignin biosynthesis

PpNAC187 Enhances Lignin Synthesis in ‘Whangkeumbae’ Pear (Pyrus pyrifolia) ‘Hard-End’ Fruit

A disorder in pears that is known as ‘hard-end’ fruit affects the appearance, edible quality, and market value of pear fruit. RNA-Seq was carried out on the calyx end of ‘Whangkeumbae’ pear fruit with and without the hard-end symptom to explore the mechanism underlying the formation of hard-end. The results indicated that the genes in the phenylpropanoid pathway affecting lignification were up-regulated in hard-end fruit. An analysis of differentially expressed genes (DEGs) identified three NAC transcription factors, and RT-qPCR analysis of PpNAC138, PpNAC186, and PpNAC187 confirmed that PpNAC187 gene expression was correlated with the hard-end disorder in pear fruit. A transient increase in PpNAC187 was observed in the calyx end of ‘Whangkeumbae’ fruit when they began to exhibit hard-end symptom. Concomitantly, the higher level of PpCCR and PpCOMT transcripts was observed, which are the key genes in lignin biosynthesis. Notably, lignin content in the stem and leaf tissues of transgenic tobacco overexpressing PpNAC187 was significantly higher than in the control plants that were transformed with an empty vector. Furthermore, transgenic tobacco overexpressing PpNAC187 had a larger number of xylem vessel elements. The results of this study confirmed that PpNAC187 functions in inducing lignification in pear fruit during the development of the hard-end disorder. View Full-Tex