The c-Abl expression in uterine epithelium during the mouse estrus cycle

WOS: 000299376400013PubMed ID: 22143492The epithelial cells of the mouse endometrium comprises the recurring physiologic changes such as proliferation and apoptosis induced by the reproductive hormones throughout the estrus cycle. The c-Abl is a protein tyrosine kinase, localized in the different cellular compartments such as nucleus, cytoplasm, mitochondria, and endoplasmic reticulum, interacts with different cellular proteins, including signaling adaptors, kinases, phosphatases, cell-cycle regulators, transcription factors and cytoskeletal proteins. Our hypothesis is that the c-Abl expression in the mouse uterine epithelium shows cyclic changes during the estrus cycle that is involved in regulation of the endometrial epithelial cells. The regulation of c-Abl gene and protein expression in the uterus of intact animals in the different cycle phases was investigated using immunohistochemistry, western blot and semi-quantitative RT-PCR. The immunohistochemistry results revealed that the luminal and the glandular epithelium (LE) and (GE), respectively, showed gradually increase in the expression of c-Abl from the proestrus (P) to the metestrus and followed by a decrease in the diestrus (D). c-Abl immunoreactivity was detected in the both LE and GE cells, especially the LE showed diminished the c-Abl protein expression on the D phase and the minimal value was detected on the P day. The c-Abl protein level in the LE was increased during the M, presenting a high correlation with the hormonal level of this cell type. The result of c-Abl RT-PCR analysis was compatible with pattern of c-Abl protein expression. In conclusion, the stage-specific protein pattern of the mammalian c-Abl tyrosine kinase presented a good correlation with the mouse estrus cycle and may have a regulative function during the uterine remodeling.Akdeniz University-Antalya/Turkey [2004.02.0122.006]This study was supported by the Research Fund of Akdeniz University-Antalya/Turkey (project #2004.02.0122.006)

p38 MAPK signal activity is critical for GLUT1 and GLUT4 expressions, maintaining pluripotency and survival of early mouse embryos

29th Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE) -- JUL 07-10, 2013 -- London, ENGLANDWOS: 000320467700398…European Soc Human Reprod & Embryol (ESHRE

Superovulation influences epab and pabpc1 gene expressions in mouse oocytes and early preimplantation embryos

29th Annual Meeting of the European-Society-of-Human-Reproduction-and-Embryology (ESHRE) -- JUL 07-10, 2013 -- London, ENGLANDWOS: 000320467700824…European Soc Human Reprod & Embryol (ESHRE