The impact of free-air CO_2 enrichment (FACE) and N supply on growth, yield and quality of rice crops with large panicle

Because CO_2 is needed for plant photosynthesis, the increase in atmospheric [CO_2] has the potential to enhance the growth and development of plant. However, the resultant effects on growth, yield and quality of field-grown rice remain unclear, especially under differing nitrogen (N) availability and/or using cultivars with large panicles. To investigate these, a Free-Air CO_2 Enrichment (FACE) experiment was performed at Wuxi, Jiangsu, China, in 2001-03. A japonica cultivar with large panicle was exposed to two [CO_2] (ambient, ambient+200μmol mol^) at three levels of N supply (15, 25, 35gNm^). FACE accelerates phenology significantly, with 3-5 days earlier in heading and 6-9 days earlier in maturity across 3 years. FACE significantly increased the grain yield by 12.8%, which was mainly due to substantially increased panicle number per square meter (+19%) as result of significant increases in tillering occurrence speed. However the spikelet number per panicle was greatly reduced (-8%), which was due mainly to the significant increase in degenerated spikelets per panicle (+52%) while differentiated spikelets per panicle showed no change. Overall DM accumulation at harvest was stimulated somewhat more (+16%) by FACE, compared to grain yield, by an average of 13% by FACE, thus resulting in 3% reduction in harvest index. FACE caused significant reduction in shoot N concentration (-7%) and significant increase in P concentration (+14%) at grain maturity, resulting in significant increase in N use efficiency and significant reduction in P use efficiency. Both shoot N uptake (+9%) and P uptake (+33%) showed significant increase at harvest, which was mainly due to significant enhanced N and P uptake during early growth stage. On a per plant basis, FACE significantly increased cumulative root volume, root dry weight, adventitious root length and adventitious root number at heading, which was mainly associated with significant increases in root growth rate during early growth period, while total surface area, active adsorption area and root oxidation activity per unit root dry weight showed significant reduction. As for grain quality, FACE cause deterioration of processing suitability and appearance quality drastically, the nutritive value of grain was also negatively influenced by FACE due to a reduction in grain protein and Cu concentration. By contrast, FACE resulted in better eating/cooking quality. For most cases, no [CO_2]×N interaction was detected for the growth, yield and quality parameters. Data from this study has important implications for fertilizer (e.g. N, P) management and variety selection in rice production systems under future elevated [CO_2] conditions.Special Revie

Design and Control of the "TransBoat": A Transformable Unmanned Surface Vehicle for Overwater Construction

This paper presents the TransBoat, a novel omnidirectional unmanned surface vehicle (USV) with a magnetbased docking system for overwater construction with wave disturbances. This is the first such USV that can build overwater structures by transporting modules. The TransBoat incorporates two features designed to reject wave disturbances. First, the TransBoat's expandable body structure can actively transform from a mono-hull into a multi-hull for stabilization in turbulent environments by extending its four outrigger hulls. Second, a real-time nonlinear model predictive control (NMPC) scheme is proposed for all shapes of the TransBoat to enhance its maneuverability and resist disturbance to its movement, based on a nonlinear dynamic model. An experimental approach is proposed to identify the parameters of the dynamic model, and a subsequent trajectory tracking test validates the dynamics, NMPC controller and system mobility. Further, docking experiments identify improved performance in the expanded form of the TransBoat compared with the contracted form, including an increased success rate (of ~ 10%) and reduced docking time (of ~ 40 s on average). Finally, a bridge construction test verifies our system design and the NMPC control method

LTe2 induces cell apoptosis in multiple myeloma by suppressing AKT phosphorylation at Thr308 and Ser473

Multiple myeloma (MM) is a highly heterogeneous hematological malignancy originating from B lymphocytes, with a high recurrence rate primarily due to drug resistance. 2-((1H-indol-3-yl)methyl)-3-((3-((1H-indol-3-yl)methyl)-1H-indol-2-yl)methyl)-1H-indole (LTe2), a tetrameric indole oligomer, possesses a wide range of anticancer activities through various mechanisms. Here, we aim to explore the anti-tumor efficiency and potential downstream targets of LTe2 in MM. Its bioactivity was assessed by employing MTT assays, flow cytometry, and the 5TMM3VT mouse model. Additionally, transcriptomic RNA-seq analysis and molecular dynamics (MD) experiments were conducted to elucidate the mechanism underlying LTe2 induced MM cell apoptosis. The results demonstrated that LTe2 significantly inhibited MM cell proliferation both in vitro and in vivo, and revealed that LTe2 exerts its effect by inhibiting the phosphorylation of AKT at the Thr308 and Ser473 sites. In summary, our findings highlight the potential of LTe2 as a novel candidate drug for MM treatment and provided a solid foundation for future clinical trials involving LTe2

Diphenyl Difluoroketone: A Potent Chemotherapy Candidate for Human Hepatocellular Carcinoma

Diphenyl difluoroketone (EF24), a molecule having structural similarity to curcumin, was recently reported to inhibit proliferation of various cancer cells significantly. Here we try to determine the effect and mechanism of EF24 on hepatocellular carcinoma. 2 µM EF24 was found to inhibit the proliferation of PLC/PRF/5, Hep3B, HepG2, SK-HEP-1 and Huh 7 cell lines. However, even 8 µM EF24 treatment did not affect the proliferation of normal liver LO2 cells. Accordingly, 20 mg/kg/d EF24 inhibited the growth of the tumor xenografts conspicuously while causing no apparent change in liver, spleen or body weight. In addition, significant apoptosis and G2/M phase cell cycle arrest were found using flow cytometry. Besides, caspases and PARP activation and features typical of apoptosis including fragmented nuclei with condensed chromatin were also observed. Furthermore, the mechanism was targeted at the reduction of nuclear factor kappa b (NF-κB) pathway and the NF-κB–regulated gene products Bcl-2, COX-2, Cyclin B1. Our study has offered a strategy that EF24 being a therapeutic agent for hepatocellular carcinoma

Hierarchical Q-Learning Based UAV Secure Communication against Multiple UAV Adaptive Eavesdroppers

In this paper, we investigate secure unmanned aerial vehicle (UAV) communication in the presence of multiple UAV adaptive eavesdroppers (AEs), where each AE can conduct eavesdropping or jamming adaptively by learning others’ actions for degrading the secrecy rate more seriously. The one-leader and multi-follower Stackelberg game is adopted to analyze the mutual interference among multiple AEs, and the optimal transmit powers are proven to exist under the existing conditions. Following that, a mixed-strategy Stackelberg Equilibrium based on finite and discretized power set is also derived and a hierarchical Q-learning based power allocation algorithm (HQLA) is proposed to obtain the optimal power allocation strategy of the transmitter. Numerical results show that secrecy performance can be degraded severely by multiple AEs and verify the availability of the optimal power allocation strategy. Finally, the effect of the eavesdropping cost on the AE’s attack mode strategies is also revealed

Impact of Prolonged Mechanical Ventilation on Ferroptosis in Renal Ischemia/Reperfusion Injury in Rats

We here investigated the impact of mechanical ventilation (MV) time on ferroptosis in a rat renal ischemia/reperfusion injury (IRI) model. Thirty-two male adult Sprague Dawley rats were divided into four groups (n=8/group): the sham group, IRI group, IRI+MV-4 h group, and IRI+MV-12 h group. Rats in the IRI group were subjected to 45 min bilateral renal ischemia. Rats in the IRI+MV groups were additionally mechanically ventilated with tracheal intubation after 45 min bilateral renal ischemia. Morphological changes associated with kidney injury and ferroptosis were assessed by hematoxylin and eosin staining and electron microscopy. Levels of the central regulator of ferroptosis, glutathione peroxidase 4 (GPX4), and lipid peroxidation markers 4-hydroxynonenal (4HNE) and superoxide dismutase 2 (SOD2) were determined in the kidney tissue by western blotting. Glutathione (GSH) levels were assessed in the serum and kidney homogenate. Scr levels in the IRI+MV-12 h group were significantly higher than those in the sham, IRI, and IRI+MV-4 h groups (all P IRI group > IRI+MV-4 h group > IRI+MV-12 h group (P<0.05 for all comparisons). By contrast, the 4HNE levels progressively increased in the kidney, with the highest values in the IRI+MV-12 h group (P<0.05, vs. the IRI group and vs. the IRI+MV-4 h group). Further, the GSH levels in the serum and kidney homogenates were significantly reduced in the IRI+MV-12 h group (P<0.01, vs. IRI group and vs. the IRI+MV-4 h group). A significant positive correlation was observed between the serum and kidney GSH levels (r2=0.542, P=0.03). These observations suggested that prolonged MV may exacerbate renal function failure, already initiated by IRI, by ferroptosis. Depletion of GSH may contribute to this effect, which requires further investigation

LncRNA NEAT1 suppresses cellular senescence in hepatocellular carcinoma via KIF11‐dependent repression of CDKN2A

Abstract Background Hepatocellular carcinoma (HCC) is the third leading cause of cancer‐related deaths worldwide. Therapeutic options for advanced HCC are limited, which is due to a lack of full understanding of pathogenesis. Cellular senescence is a state of cell cycle arrest, which plays important roles in the pathogenesis of HCC. Mechanisms underlying hepatocellular senescence are not fully understood. LncRNA NEAT1 acts as an oncogene and contributes to the development of HCC. Whether NEAT1 modulates hepatocellular senescence in HCC is unknown. Methods The role of NEAT1 and KIF11 in cellular senescence and tumor growth in HCC was assessed both in vitro and in vivo. RNA pulldown, mass spectrometry, Chromatin immunoprecipitation (ChIP), luciferase reporter assays, RNA FISH and immunofluorescence (IF) staining were used to explore the detailed molecular mechanism of NEAT1 and KIF11 in cellular senescence of HCC. Results We found that NEAT1 was upregulated in tumor tissues and hepatoma cells, which negatively correlated with a senescence biomarker CDKN2A encoding p16INK4a and p14ARF proteins. NEAT1 was reduced in senescent hepatoma cells induced by doxorubicin (DOXO) or serum starvation. Furthermore, NEAT1 deficiency caused senescence in cultured hepatoma cells, and protected against the progression of HCC in a mouse model. During senescence, NEAT1 translocated into cytosol and interacted with a motor protein KIF11, resulting in KIF11 protein degradation and subsequent increased expression of CDKN2A in cultured hepatoma cells. Furthermore, KIF11 knockdown caused senescence in cultured hepatoma cells. Genetic deletion of Kif11 in hepatocytes inhibited the development of HCC in a mouse model. Conclusions Conclusively, NEAT1 overexpression reduces senescence and promotes tumor progression in HCC tissues and hepatoma cells, whereas NEAT1 deficiency causes senescence and inhibits tumor progression in HCC. This is associated with KIF11‐dependent repression of CDKN2A. These findings lay the foundation to develop potential therapies for HCC by inhibiting NEAT1 and KIF11 or inducing senescence