Quantitative regulation of FLC via coordinated transcriptional initiation and elongation

The basis of quantitative regulation of gene expression is still poorly understood. In Arabidopsis thaliana, quantitative variation in expression of FLOWERING LOCUS C (FLC) influences the timing of flowering. In ambient temperatures, FLC expression is quantitatively modulated by a chromatin silencing mechanism involving alternative polyadenylation of antisense transcripts. Investigation of this mechanism unexpectedly showed that RNA polymerase II (Pol II) occupancy changes at FLC did not reflect RNA fold changes. Mathematical modeling of these transcriptional dynamics predicted a tight coordination of transcriptional initiation and elongation. This prediction was validated by detailed measurements of total and chromatin-bound FLC intronic RNA, a methodology appropriate for analyzing elongation rate changes in a range of organisms. Transcription initiation was found to vary ∼25-fold with elongation rate varying ∼8- to 12-fold. Premature sense transcript termination contributed very little to expression differences. This quantitative variation in transcription was coincident with variation in H3K36me3 and H3K4me2 over the FLC gene body. We propose different chromatin states coordinately influence transcriptional initiation and elongation rates and that this coordination is likely to be a general feature of quantitative gene regulation in a chromatin context

Accurate Guidance Method and App Development for Assigning Parking Spaces Based on Indoor Wi-Fi

Existing parking guidance systems only provide road guidance outside the parking lot but do not provide accurate guidance to specific parking spaces inside the parking lot. By using a Kalman filter, the Grubbs test, and a neural network algorithm to improve the RSSI-based location fingerprint identification technology, an accurate location method based on indoor Wi-Fi is obtained, which implements precise route guidance and a reverse search function for parking spaces. We utilize Beidou positioning to develop a Gaode map for outdoor navigation and use an integrated system of ultrasonic detector/indicators and ground locks to manage parking spaces. Through the secondary development of an Android system and the application of a MySql database, an app for precise parking guidance was developed. The system makes full use of the Internet and parking information, eliminates information asymmetry, improves the utilization ratio of the urban static traffic resources, allocates parking spaces in real-time, breaks information islands, provides parking search and recommendation functions for users, achieves parking information-sharing, and effectively improves parking efficiency and the parking utilization ratio

Suppression of FM-to-AM conversion in third-harmonic generation at the retracing point of a crystal

FM-to-AM conversion can cause many negative effects (e.g., reducing of margin against damage to the optics, and etc.) on performances of third-harmonic conversion system. In this letter, the FM-to-AM conversion effect in third-harmonic generation is investigated both at and away from the retracing point of type-II KDP crystal. Obtained results indicate that the FM-to-AM conversion can be suppressed effectively when the crystal works at the retracing point.Comment: 8 pages, 5 figures, 1 tabl

Discovery and identification of potential biomarkers of papillary thyroid carcinoma

<p>Abstract</p> <p>Background</p> <p>Thyroid carcinoma is the most common endocrine malignancy and a common cancer among the malignancies of head and neck. Noninvasive and convenient biomarkers for diagnosis of papillary thyroid carcinoma (PTC) as early as possible remain an urgent need. The aim of this study was to discover and identify potential protein biomarkers for PTC specifically.</p> <p>Methods</p> <p>Two hundred and twenty four (224) serum samples with 108 PTC and 116 controls were randomly divided into a training set and a blind testing set. Serum proteomic profiles were analyzed using SELDI-TOF-MS. Candidate biomarkers were purified by HPLC, identified by LC-MS/MS and validated using ProteinChip immunoassays.</p> <p>Results</p> <p>A total of 3 peaks (<it>m/z </it>with 9190, 6631 and 8697 Da) were screened out by support vector machine (SVM) to construct the classification model with high discriminatory power in the training set. The sensitivity and specificity of the model were 95.15% and 93.97% respectively in the blind testing set. The candidate biomarker with <it>m/z </it>of 9190 Da was found to be up-regulated in PTC patients, and was identified as haptoglobin alpha-1 chain. Another two candidate biomarkers (6631, 8697 Da) were found down-regulated in PTC and identified as apolipoprotein C-I and apolipoprotein C-III, respectively. In addition, the level of haptoglobin alpha-1 chain (9190 Da) progressively increased with the clinical stage I, II, III and IV, and the expression of apolipoprotein C-I and apolipoprotein C-III (6631, 8697 Da) gradually decreased in higher stages.</p> <p>Conclusion</p> <p>We have identified a set of biomarkers that could discriminate PTC from non-cancer controls. An efficient strategy, including SELDI-TOF-MS analysis, HPLC purification, MALDI-TOF-MS trace and LC-MS/MS identification, has been proved successful.</p

Experimental study and mechanism analysis of high-pressure water jet for mud cake cutting during shield tunneling

When the opening rate of the cutter head of tunnel boring machines is insufficient for the removal of excavated soil in a timely manner, the soil tends to accumulate in front of the cutter head and inside the earth or slurry chamber, leading to mud caking. High-pressure water jetting is an effective method for removing mud cakes. This study explored the influence of high-pressure water jet parameters on the efficiency of mud cake cleaning by using highly weathered argillaceous siltstone as experimental materials. Mud cake compaction equipment and high-pressure water jetting devices were developed. In addition, the impact of jetting parameters such as jet pressure and flow rate on the mud cake cutting performance was investigated. The results indicated that with an increase in the erosion distance, the cutting width of the mud cake first increased and then gradually decreased to zero, while the cutting depth progressively diminished. Under the same jet pressure, when the flow rate increased from 49.1 L/min to 110 L/min, the cutting width of the high-pressure water jet increased. With further increase in the flow rate from 110 L/min to 202.8 L/min, the cutting width decreased and the cutting depth increased. Under the same jet flow, the increase in water pressure resulted in greater cutting width and depth

Improving CO2 photoconversion with ionic liquid and Co single atoms

Photocatalytic CO2 conversion promises an ideal route to store solar energy into chemical bonds. However, sluggish electron kinetics and unfavorable product selectivity remain unresolved challenges. Here, an ionic liquid, 1-ethyl-3-methylimidazolium tetrafluoroborate, and borate-anchored Co single atoms were separately loaded on ultrathin g-C3N4 nanosheets. The optimized nanocomposite photocatalyst produces CO and CH4 from CO2 and water under UV-vis light irradiation, exhibiting a 42-fold photoactivity enhancement compared with g-C3N4 and nearly 100% selectivity towards CO2 reduction. Experimental and theoretical results reveal that the ionic liquid extracts electrons and facilitates CO2 reduction, whereas Co single atoms trap holes and catalyze water oxidation. More importantly, the maximum electron transfer efficiency for CO2 photoreduction, as measured with in-situ μs-transient absorption spectroscopy, is found to be 35.3%, owing to the combined effect of the ionic liquid and Co single atoms. This work offers a feasible strategy for efficiently converting CO2 to valuable chemicals

Design of hazardous materials transportation safety management system under the vehicle-infrastructure connected environment

Purpose – For the purpose of reducing the incidence of hazardous materials transport accident, eliminating the potential threats and ensuring their safety, aiming at the shortcomings in the process of current hazardous materials transportation management, this paper aims to construct the framework of hazardous materials transportation safety management system under the vehicle-infrastructure connected environment. Design/methodology/approach – The system takes the intelligent connected vehicle as the main supporter, integrating GIS, GPS, eye location, GSM, networks and database technology. Findings – By analyzing the transportation characteristics of hazardous materials, this system consists of five subsystems, which are vehicle and driver management subsystem, dangerous sources and hazardous materials management subsystem, route analysis and optimization subsystem, early warning and emergency rescue management subsystem, and basic information query subsystem. Originality/value – Hazardous materials transportation safety management system includes omnibearing real-time monitoring, timely updating of system database, real-time generation and optimization of emergency rescue route. The system can reduce the transportation cost and improve the ability of accident prevention and emergency rescue of hazardous materials