Research on the development of carrier intelligent cloud network under the background of IPv6+

With the increasingly mature 5G technology in our country, the government has comprehensively promoted IPv6 scale deployment, the rapid improvement of network quality of the three operators, and gradually transformed to IPv6+, the carrying network is more fl exible, and the user opening service is more convenient, which has promoted the development of intelligent cloud network of China’s carriers. Operators should actively respond to the challenges of IPv6+ era, based on their own intelligent cloud network development needs, the use of SRv6 technology, promote cloud network integration, carrying a variety of online services; Provide integrated cloud network products and services, build an intelligent operation and maintenance system, and improve user satisfaction; To build IPv6 networking capability of the whole network and build intelligent cloud network; Do a good job in the construction of IPv6 network information security, improve the security defense capability of intelligent cloud network, ensure the smooth operation of network, and inject new vitality into the 2B industry market for operators

Reconfi guration analysis of carrier security system for IPv6

With the continuous development of the Internet, IPv6 planning and deployment has become the current “accelerate the construction of network power, to win the future international competition of new advantages” an urgent requirement, and the current security system reconstruction in the implementation of IPv6 planning and deployment plan has a very important practical promotion signifi cance. However, we should pay attention to one point is that compared with the Internet construction and operation and maintenance, the operator IPv6 network construction and its security operation and maintenance is a certain difference. Therefore, based on the background of the new era, it is very necessary to actively explore the path of IPv6 oriented carrier security system reconstruction planning. Based on this, this paper fi rst describes the application advantages of IPv6, and briefl y analyzes the status quo of the carrier security system, and discusses the reconstruction direction and planning of the IPv6 oriented carrier security system, for reference only

The nonequilibrium evolution near the phase boundary

We study the nonequilibrium evolution near the phase boundary of the 3D Ising model, and find that the average of relaxation time (RT) near the first-order phase transition line (1st-PTL) is significantly larger than that near the critical point (CP). As the system size increases, the average of RT near the 1st-PTL increases at a higher power compared to that near the CP. We further show that RT near the 1st-PTL is not only non-self-averaging, but actually self-diverging: relative variance of RT increases with system size. The presence of coexisting and metastable states results in a substantial increase in randomness near the 1st-PTL, making it difficult to achieve equilibrium.Comment: 6 pages, 3 figure

Growth of Human Colorectal Cancer SW1116 Cells Is Inhibited by Cytokine-Induced Killer Cells

Previous reports have suggested that treatment with cytokine-induced killer (CIK) cells may benefit patients with various types of tumor. The aim of this study was to evaluate the antitumor effects of CIK cells against the colorectal cancer line SW1116 in vitro and in vivo. CIK cells were generated routinely from peripheral blood mononuclear cells of healthy human donors, and the number of CD3+CD56+ cells was expanded more than 1300-fold after 14-day culture. At an effector : target cell ratio of 50 : 1, the percentage lysis of SW1116 cells reached 68% in the presence of CIK cells, Experimental mice injected with SW1116 cells subcutaneously were divided randomly into four groups: untreated, 5-fluorouracil (5-FU)-treated, CIK-consecutive treated (injected once/day) and CIK-interval treated (injected once every 5 days). CIK cells were injected abdominally five times in total. Compared with the untreated group, xenograft growth was inhibited greatly by CIK treatment, to nearly the same extent as with 5-FU treatment. We demonstrated that the necrotic area in the tumor xenograft was markedly larger in the CIK-treated groups than in the other groups. These findings suggest that CIK-based immunotherapy may represent an effective choice for patients with colorectal cancer

Id2 promotes the invasive growth of MCF-7 and SKOV-3 cells by a novel mechanism independent of dimerization to basic helix-loop-helix factors

<p>Abstract</p> <p>Background</p> <p>Inhibitor of differentiation 2 (<it>Id2</it>) is a critical factor for cell proliferation and differentiation in normal vertebrate development. Most of the biological function of Id2 has been ascribed to its helix-loop-helix motif. Overexpression of Id2 is frequently observed in various human tumors, but its role for invasion potential in tumor cells is dispute. We aimed to reveal the role of Id2 in invasion potential in poorly invasive and estrogen receptor α (ERα)-positive MCF-7 and SKOV-3 cancer cells.</p> <p>Methods</p> <p>MCF-7 and SKOV-3 cells were stably transfected with the wild-type, degradation-resistant full-length or helix-loop-helix (HLH)-deleted Id2, respectively. Protein levels of Id2 and its mutants and E-cadherin were determined by western blot analysis and mRNA levels of Id2 and its mutants were determined by RT-PCR. The effects of Id2 and its mutants on cell proliferation were determined by [³H]-thymidine incorporation assay and the 3- [4, 5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide (MTT) dye method. The <it>in vitro </it>invasion potential of cells was evaluated by Transwell assay. Cell motility was assessed by scratch wound assay. The promoter activity of <it>E-cadherin </it>was determined by cotransfection and luciferase assays.</p> <p>Results</p> <p>Ectopic transfection of the wild-type Id2 markedly increased the protein and mRNA expression of <it>Id2 </it>in MCF-7 and SKOV-3 cells; the protein level but not mRNA level was further increased by transfection with the degradation-resistant Id2 form. The ectopic expression of Id2 or its mutants did not alter proliferation of either MCF-7 or SKOV-3 cells. Transfection of the wild-type Id2 significantly induced the invasion potential and migratory capacity of cells, which was further augmented by transfection with the degradation-resistant full-length or HLH-deleted Id2. E-cadherin protein expression and transactivation of the proximal E-cadherin promoter were markedly suppressed by the degradation-resistant full-length or HLH-deleted Id2 but not wild-type Id2. Ectopic expression of E-cadherin in MCF-7 and SKOV-3 cells only partially blunted the invasion potential induced by the degradation-resistant HLH-deleted Id2.</p> <p>Conclusion</p> <p>Overexpression of Id2 in ERα-positive epithelial tumor cells indeed increases the cells' invasive potential through a novel mechanism independent of dimerization to basic helix-loop-helix factors. E-cadherin contributes only in part to Id2-induced cell invasion when Id2 is accumulated to a higher level in some specific cell types.</p

Investigations into the characteristics and influences of nonequilibrium evolution

In order to estimate qualitatively the influence of nonequilibrium evolution in relativistic heavy ion collisions, we use the three dimensional Ising model with Metropolis algorithm to study the evolution from nonequilibrium to equilibrium on the phase boundary. The evolution of order parameter approaches its equilibrium value exponentially, the same as that given by Langevin equation. The average relaxation time is defined which is demonstrated to well represent the relaxation time in dynamical equations. It is shown that the average relaxation time at critical temperature diverges as the zth power of system size. The third and the fourth cumulants of order parameter during the nonequilibrium evolution could be either positive or negative, depending on the observation time, consistent with dynamical models at T > Tc. It is found that the nonequilibrium evolution at T > Tc lasts very short, and the influence is weaker than that at T < Tc. Those qualitative features are instructive to determine experimentally the critical point and the phase boundary of QCD.Comment: 8 pages, 5 figures; add fig.3 and up date the eq.(9-10), and content