Comparative mitogenomic analyses of three scallops (Bivalvia: Pectinidae) reveal high level variation of genomic organization and a diversity of transfer RNA gene sets

<p>Abstract</p> <p>Background</p> <p>It can be seen from the available mollusk mitogenomes that the family Pectinidae exhibits the most variation in genome organization. In this study, comparative mitogenomic analyses were performed for three scallops from the subfamily Chlamydinae (Pectinidae), with the goal of characterizing the degree of variability of mitogenome organization and other characteristics among species from the same subfamily and exploring their possible evolution route.</p> <p>Findings</p> <p>The complete or nearly complete mtDNA sequences of scallop <it>Mimachlamys nobilis </it>(17 935 bp), <it>Mizuhopecten yessoensis </it>(20 964 bp) and <it>Chlamys farreri </it>(17 035 bp) were determined using long PCR amplification and primer walking sequencing strategy. Highly variable size difference of the three genomes resulted primarily from length and number variations of non-coding regions, and the major difference in gene content of the three scallop species are due to varying tRNA gene sets. Only 21, 16, and 17 tRNA genes were detected in the mitogenomes of <it>M. nobilis</it>, <it>M. yessoensis </it>and <it>C. farreri</it>, respectively. Remarkably, no <it>trnS </it>gene could be identified in any of the three scallops. A newly-detected <it>trnA</it>-like sequence within the mitogenome of <it>M. yessoensis </it>seems to exemplify the functional loss of a tRNA gene, and the duplication of <it>trnD </it>in <it>M. yessoensis </it>raises a fundamental question of whether the retention of the tRNA gene copy of 2-tRNAs is easier than that of 4-tRNAs. Analysis of putative evolutionary pathways of gene rearrangement indicates that transposition of neighboring gene blocks may play an important role in the evolution of mitogenomes in scallops. Parsimonious analysis of the genomic variations implies that the mitogenomes of <it>M. yessoensis </it>and <it>C. farreri </it>are likely to derive independently from a common ancestor that was closely related to <it>M. nobilis</it>.</p> <p>Conclusion</p> <p>Comparative mitogenomic analyses among three species from the subfamily Chlamydinae show that the three genomes exhibit a high level of genomic variation and a diversity of tRNA gene sets, characterized by extensive translocation of genes. These features provide useful clues and information for evolutionary analysis of scallop mitogenomes.</p

Association of renin–angiotensin system inhibitors use with short- and long-term mortality in patients with aortic stenosis: A systematic review and meta-analysis

PurposeThe present study aimed to investigate the association of renin–angiotensin system inhibitors (RASi) with short- and long-term mortality in patients with aortic stenosis (AS).MethodsA systematic search was performed in PubMed, Embase, and Cochrane library databases for relevant studies published before March 2022. Studies meeting the inclusion criteria were included to assess the effect of RASi on short-term (≤30 days) and long-term (≥1 year) mortality in patients with AS.ResultsA total of 11 studies were included in the meta-analysis. Our results demonstrated that RASi reduced short-term mortality (OR = 0.76, 95% CI 0.63–0.93, p = 0.008) after aortic valve replacement (AVR). Subgroup analysis revealed that RASi was still associated with lower short-term mortality after transcatheter aortic valve replacement (TAVR); however, the association was relatively weak in patients who underwent surgical aortic valve replacement (SAVR). For long-term mortality, the pooled OR was 1.04 (95% CI 0.88–1.24, p = 0.63) after sensitivity analysis in patients who did not undergo AVR. In addition, our study confirmed that RASi significantly reduced long-term mortality (OR = 0.57, 95% CI 0.44–0.74, p &lt; 0.0001) in patients who underwent AVR. Subgroup analysis showed that both TAVR and SAVR groups treated with RASi had lower long-term mortality.ConclusionRenin–angiotensin system inhibitors did not change long-term mortality in AS patients who did not undergo AVR. However, RASi reduced short- and long-term mortality in patients who underwent AVR

Remnant cholesterol is associated with cardiovascular mortality

Background: Genetic, observational, and clinical intervention studies indicate that circulating levels of remnant cholesterol (RC) are associated with cardiovascular diseases. However, the predictive value of RC for cardiovascular mortality in the general population remains unclear. Methods: Our study population comprised 19,650 adults in the United States from the National Health and Nutrition Examination Survey (NHANES) (1999–2014). RC was calculated from non-high-density lipoprotein cholesterol (non-HDL-C) minus low-density lipoprotein cholesterol (LDL-C) determined by the Sampson formula. Multivariate Cox regression, restricted cubic spline analysis, and subgroup analysis were applied to explore the relationship of RC with cardiovascular mortality. Results: The mean age of the study cohort was 46.4 ± 19.2 years, and 48.7% of participants were male. During a median follow-up of 93 months, 382 (1.9%) cardiovascular deaths occurred. In a fully adjusted Cox regression model, log RC was significantly associated with cardiovascular mortality [hazard ratio (HR) 2.82; 95% confidence interval (CI) 1.17–6.81]. The restricted cubic spline curve indicated that log RC had a linear association with cardiovascular mortality (p for non-linearity = 0.899). People with higher LDL-C (≥130 mg/dL), higher RC [≥25.7/23.7 mg/dL in males/females corresponding to the LDL-C clinical cutoff point (130 mg/dL)] and abnormal HDL-C (<40/50 mg/dL in males/females) levels had a higher risk of cardiovascular mortality (HR 2.18; 95% CI 1.13–4.21 in males and HR 2.19; 95% CI 1.24–3.88 in females) than the reference group (lower LDL-C, lower RC and normal HDL-C levels). Conclusions: Elevated RC levels were associated with cardiovascular mortality independent of traditional risk factors

Apatinib combined with camrelizumab in the treatment of recurrent/metastatic nasopharyngeal carcinoma: a prospective multicenter phase II study

BackgroundPreclinical studies demonstrated that immune checkpoint inhibitors combined with antiangiogenic drugs have a synergistic anti-tumor effect. This present phase II trial aimed to evaluate the efficacy and safety of apatinib combined with camrelizumab in patients with recurrent/metastatic nasopharyngeal carcinoma (RM-NPC).MethodsPatients with RM-NPC were administered with apatinib at 250 mg orally once every day and with camrelizumab at 200 mg via intravenous infusion every 2 weeks until the disease progressed or toxicity became unacceptable. The objective response rate (ORR) was the primary endpoint, assessed using RECIST version 1.1. Progression-free survival (PFS), overall survival (OS), disease control rate (DCR) and safety were the key secondary endpoints. This study was registered with ClinicalTrials.gov, NCT04350190.ResultsThis study enrolled 26 patients with RM-NPC between January 14, 2021 and September 15, 2021. At data cutoff (March 31, 2023), the median duration of follow-up was 16 months (ranging from 1 to 26 months). The ORR was 38.5% (10/26), the disease control rate (DCR) was 61.5% (16/26), and the median PFS was 6 months (IQR 3.0-20.0). The median OS was 14 months (IQR 6.0-21.25). Treatment-related grade 3 or 4 adverse events occurred in seven (26.9%) patients, and comprised anemia (7.7%), stomatitis (3.8%), headache (3.8%), pneumonia (7.7%), and myocarditis (3.8%). There were no serious treatment-related adverse events or treatment-related deaths.ConclusionIn patients with RM-NPC, apatinib plus camrelizumab showed promising antitumor activity and manageable toxicities

X-ray Polarimetry of the accreting pulsar 1A~0535+262 in the supercritical state with PolarLight

The X-ray pulsar 1A 0535+262 exhibited a giant outburst in 2020, offering us a unique opportunity for X-ray polarimetry of an accreting pulsar in the supercritical state. Measurement with PolarLight yielded a non-detection in 3-8 keV; the 99% upper limit of the polarization fraction (PF) is found to be 0.34 averaged over spin phases, or 0.51 based on the rotating vector model. No useful constraint can be placed with phase resolved polarimetry. These upper limits are lower than a previous theoretical prediction of 0.6-0.8, but consistent with those found in other accreting pulsars, like Her X-1, Cen X-3, 4U 1626-67, and GRO J1008-57, which were in the subcritical state, or at least not confidently in the supercritical state, during the polarization measurements. Our results suggest that the relatively low PF seen in accreting pulsars cannot be attributed to the source not being in the supercritical state, but could be a general feature.Comment: accepted for publication in Ap

Intelligent optimal scheduling strategy of IES with considering the multiple flexible loads

As the energy system continues to evolve and advance, a relatively single energy system gradually turns to the comprehensive energy system. With the advancement of technology related to electric vehicle networks progresses, the flexible load in the power system has been significantly improved, and there will be more and more schedulable resources in the power system. On this basis, the paper mainly studies the integrated energy system including the power station of electric driven vehicles and a variety of flexible loads. First, an intelligent energy system model is built, including wind power, ES, cogeneration units, gas boilers, etc. Secondly, according to the flexible load response of electricity, heat and gas, the dynamic response of flexible loads such as electricity, heat and natural gas is incorporated into the optimal scheduling of the integrated energy system, and a model has been created for optimizing the integrated energy system with the goal of minimizing the system operational expenses. Finally, YALMIP is utilized to formulate the problem during modeling, while CPLEX is employed as the solver to find a solution. Through the example calculation, the system operation cost is reduced by 13.04%, the wind power consumption rate is increased by 8.65%, and the peak valley difference is reduced by 24.58%. This approach has been demonstrated to lower the overall operational expenses of the system, as well as assist in smoothing out peak and off-peak electricity demand, and increase the utilization of wind power

Magnetotelluric Evidence for Lithospheric Alteration Beneath the Wuyi‐Yunkai Orogen: Implications for Thermal Structure of South China

Abstract The Wuyi‐Yunkai Orogen experienced a polyphase tectonomagmatism and is a key region for deciphering the alteration and thermal structure of the South China Block lithosphere. Herein, an electrical resistivity model of the lithosphere is presented via the three‐dimensional inversion of broadband (0.003–3600 s) magnetotelluric (MT) data collected along a 380‐km‐long profile comprising 62 MT sites across the Wuyi‐Yunkai Orogen, and the robustness of this model is critically evaluated through a series of sensitivity tests. The resistivity model reveals that the upper crust of the Cathaysia Block and the Wuyi‐Yunkai Orogen is dominated by high‐resistivity sedimentary cover interposed with low‐resistivity features, mainly along fault zones. High‐resistivity bodies and strong conductors in the upper crust are interpreted as magmatic rocks and tectonic mélanges, respectively. Another feature of this resistivity model is the presence of zones featuring enhanced electrical conductivity (<30 Ωm) extending from the lower crust to the upper mantle beneath the Wuyi‐Yunkai Orogen. The conductors in the lower crust are attributed to saline fluids from either the dehydration of the subducting Paleo‐Pacific slab or the regional metamorphism‐induced dehydration of sandy argillaceous rocks. In contrast, the conductors in the upper mantle are attributed to 4%–7% partial melt, which corresponds to the analyses of mantle xenoliths in South China. These conductors in the upper crust and upper mantle supply volatiles and heat to shallow geothermal systems. This work ultimately shows that the lithospheric thinning of South China is controlled mainly by mantle upwelling caused by the retreat of the subducting Paleo‐Pacific slab