Benzylammonium hexanoate

A binary mixture of benzylamine and hexanoic acid has been reacted to form the title salt, C7H10N+·C6H11O2-. This crystal has a 1:1 stoichiometry of acid- and amine-derived species which contrasts with other related species which can have a number of other integer ratios of acid and amine components. The diffraction data indicate complete transfer of a proton from the acid to the amine to give the salt, comprising a cation and anion combination, with the formation of three hydrogen bonds around each ammonium group. This contrasts with other related species.This work was supported by the Department of Chemistry, the BP Institute and the Oppenheimer Trust

Glucagon-like peptide-2 modulates neurally evoked mucosal chloride secretion in guinea pig small intestine in vitro

Glucagon-like peptide-2 (GLP-2) is an important neuroendocrine peptide in intestinal physiology. It influences digestion, absorption, epithelial growth, motility, and blood flow. We studied involvement of GLP-2 in intestinal mucosal secretory behavior. Submucosal-mucosal preparations from guinea pig ileum were mounted in Ussing chambers for measurement of shortcircuit current (Isc) as a surrogate for chloride secretion. GLP-2 action on neuronal release of acetylcholine was determined with ELISA. Enteric neuronal expression of the GLP-2 receptor (GLP-2R) was studied with immunohistochemical methods. Application of GLP-2 (0.1–100 nM) to the serosal or mucosal side of the preparations evoked no change in baseline Isc and did not alter transepithelial ionic conductance. Transmural electrical field stimulation (EFS) evoked characteristic biphasic increases in Isc, with an initially rapid rising phase followed by a sustained phase. Application of GLP-2 reduced the EFS-evoked biphasic responses in a concentration-dependent manner. The GLP-2R antagonist GLP-2-(3-33) significantly reversed suppression of the EFS-evoked responses by GLP-2. Tetrodotoxin, scopolamine, and hexamethonium, but not vasoactive intestinal peptide type 1 receptor (VPAC1) antagonist abolished or reduced to near zero the EFS-evoked responses. GLP-2 suppressed EFS-evoked acetylcholine release as measured by ELISA. Pretreatment with GLP-2- (3-33) offset this action of GLP-2. In the submucosal plexus, GLP-2R immunoreactivity (-IR) was expressed in choline acetyltransferase-IR neurons, somatostatin-IR neurons, neuropeptide Y-IR neurons, and vasoactive intestinal peptide-IR neurons. We conclude that submucosal neurons in the guinea pig ileum express GLP-2R. Activation of GLP-2R decreases neuronally evoked epithelial chloride secretion by suppressing acetylcholine release from secretomotor neurons

Neutron Reflectometry for Studying Corrosion and Corrosion Inhibition

Neutron reflectometry is an extremely powerful technique to monitor chemical and morphological changes at interfaces at the angstrom-level. Its ability to characterise metal, oxide and organic layers simultaneously or separately and in situ makes it an excellent tool for fundamental studies of corrosion and particularly adsorbed corrosion inhibitors. However, apart from a small body of key studies, it has yet to be fully exploited in this area. We present here an outline of the experimental method with particular focus on its application to the study of corrosive systems. This is illustrated with recent examples from the literature addressing corrosion, inhibition and related phenomena

Xeroderma pigmentosum: a case report and review of the literature

Inherited molecular defects in nucleotide excision repair genes cause the autosomal recessive condition xeroderma pigmentosum. Xeroderma pigmentosum is characterized by photo-hyper- sensitivity of sun-exposed tissues, and by a several thousand-fold increase in the risk of developing malignant neoplasms of the skin and of the eyes. Mutations in xeroderma pigmentosum genes that regulate nucleotide excision repair, not only predispose persons with xeroderma pigmentosum to multiple malignancies, but also promote premature cutaneous and ocular ageing, and in some cases promote progressive neurodegenerative changes. This paper describes a case of xeroderma pigmentosum with advanced cutaneous squamous cell carcinoma, actinic cheilitis and ocular lesions in a 19-year old black woman. The extensive ultraviolet radiation-induced skin and eye damage are evidence of neglect of sun-protection and lack of appropriate medical care from childhood

Surface Chemistry of Almandine Garnet

Almandine garnet is used commonly in abrasive blasting processes to prepare metallic surfaces for painting. However, there is evidence that the process leads to significant amounts of abrasive embedded in the “cleaned” surface, and hence the surface chemistry of garnet will affect the binding of any coatings subsequently applied. In addition, in marine environments seawater aerosol droplets are expected to impact the exposed surface prior to coating application, depositing both water and dissolved inorganic ions. In this work, we provide indepth analysis of the chemistry of the almandine garnet surface using angle-resolved X-ray photoelectron spectroscopy (XPS) and note a correlation for several elemental oxides between literature values of the binding energy of the surface oxygen 1s XPS peak and the basicity of the hydroxyl group that forms on the surface. We also consider the adsorption to almandine garnet powder of seawater-relevant inorganic ions (sodium, magnesium, and calcium): Binding constants have been determined using titration measurements, solution-depletion isotherms, and numerical modeling, with calcium observed to bind more strongly than magnesium. The relevance of Langmuir-type fits to constant-pH adsorption isotherms is discussed. By contrast, sodium either binds very weakly or is effectively inert toward the garnet surface under the experimental conditions. The complex adsorption behavior observed emphasizes the necessity of using multiple techniques to characterize mineral surfaces

Turbulent flow at 190 m height above London during 2006-2008: A climatology and the applicability of similarity theory

Flow and turbulence above urban terrain is more complex than above rural terrain, due to the different momentum and heat transfer characteristics that are affected by the presence of buildings (e.g. pressure variations around buildings). The applicability of similarity theory (as developed over rural terrain) is tested using observations of flow from a sonic anemometer located at 190.3 m height in London, U.K. using about 6500 h of data. Turbulence statistics—dimensionless wind speed and temperature, standard deviations and correlation coefficients for momentum and heat transfer—were analysed in three ways. First, turbulence statistics were plotted as a function only of a local stability parameter z/Λ (where Λ is the local Obukhov length and z is the height above ground); the σ_i/u_* values (i = u, v, w) for neutral conditions are 2.3, 1.85 and 1.35 respectively, similar to canonical values. Second, analysis of urban mixed-layer formulations during daytime convective conditions over London was undertaken, showing that atmospheric turbulence at high altitude over large cities might not behave dissimilarly from that over rural terrain. Third, correlation coefficients for heat and momentum were analyzed with respect to local stability. The results give confidence in using the framework of local similarity for turbulence measured over London, and perhaps other cities. However, the following caveats for our data are worth noting: (i) the terrain is reasonably flat, (ii) building heights vary little over a large area, and (iii) the sensor height is above the mean roughness sublayer depth

Colorimetric Measurement of Triglycerides Cannot Provide an Accurate Measure of Stored Fat Content in Drosophila

Drosophila melanogaster has recently emerged as a useful model system in which to study the genetic basis of regulation of fat storage. One of the most frequently used methods for evaluating the levels of stored fat (triglycerides) in flies is a coupled colorimetric assay available as a kit from several manufacturers. This is an aqueous-based enzymatic assay that is normally used for measurement of mammalian serum triglycerides, which are present in soluble lipoprotein complexes. In this short communication, we show that coupled colorimetric assay kits cannot accurately measure stored triglycerides in Drosophila. First, they fail to give accurate readings when tested on insoluble triglyceride mixtures with compositions like that of stored fat, or on fat extracted from flies with organic solvents. This is probably due to an inability of the lipase used in the kits to efficiently cleave off the glycerol head group from fat molecules in insoluble samples. Second, the measured final products of the kits are quinoneimines, which absorb visible light in the same wavelength range as Drosophila eye pigments. Thus, when extracts from crushed flies are assayed, much of the measured signal is actually due to eye pigments. Finally, the lipoprotein lipases used in colorimetric assays also cleave non-fat glycerides. The glycerol backbones liberated from all classes of glycerides are measured through the remaining reactions in the assay. As a consequence, when these assay kits are used to evaluate tissue extracts, the observed signal actually represents the amount of free glycerols together with all types of glycerides. For these reasons, findings obtained through use of coupled colorimetric assays on Drosophila samples must be interpreted with caution. We also show here that using thin-layer chromatography to measure stored triglycerides in flies eliminates all of these problems