Serological and molecular characterization of begomoviruses infecting cassava (Manihot esculenta Crantz) in Africa

[no abstract

Molecular Characterization of a New Begomovirus Infecting Tobacco in Kagoshima,Japan : an Evidence for Interspecific Recombination

A virus infected tobacco sample (KG5Tob) showing leaf curl symptoms was collected in Kagashima, Japan. The complete DNA-A like molecule of KG5Tob was determined and found to comprise 2762 nucleotides with a typical begomovirus genome organization. A comparison of the DNA-A sequences revealed that KG5Tob shared 87% with its closest relatives, Honeysuckle yellow vein mosaic virus (HYVMV) and Tobacco leaf curl Yamaguchi virus (TbLCYV), however, on comparison of the intergenic region (IR) nt sequences, the isolate shared only 66 and 64% of the IR sequences eith the former and the latter, respectively. Phylogenetic analysis of full length DNA-A component clustered KG5Tob in a lone cluster but close to HYVMV and TbLCYV. When the CPs werealogned, similar results were observed and KG5Tob wa placed in a lone cluster. A comparison of the Other ORFs showed that different parts of the genome had different affinities, suggesting that recombination could have played a major role in the evolution of this isolate. Molecular data show that KG5Tob isolate is an isolate of HYVMV, although the isolate was obtained from tobacco

Molecular Characterization of a New Begomovirus Infecting Honeysuckle in Sapporo,Japan

A virus isolated from honeysuckle collected from Sapporo (SP1), Japan, showing symptoms of complete leaf yellowing and stunting was characterized. The complete DNA-A like molecules of SP1 was determined and found to comprise 2771 nucleotides (nts) with a typical begomovirus genome organization. Additionally, SP1 had a DNAβ deletion mutant molecule of 426 nts. A comparison of the complete DNA-A nt sequences revealed that SP1shared 87-88% nt sequence identity with the other three isolates of Japanese Honeysuckle yellow vein mosaic virus (HYVMV) but 91% with HYVMV-[UK1], however, the isolate shared 95-98% of CP aa sequence identity with the Japanese isolates of HYVMV and only 86% with HYVMV-[UK1]. Phylogenetic analysis of full length DNA-A component clustered SP1 with Japanese tobacco begomoviruses but analysis of the aligned CP aa sequences clustered the virus with the Japanese honeysuckle begomoviruses. Molecular data show that P1 is an isolate of HYVMV

Molecular Characterization of a New Begomovirus Infecting Honeysuckle in Kobe,Japan

A virus (HY12) isolated from honeysuckle (Lonicera japonica Thunb.) collected in Kobe,Hyogo prefecture, Japan, showing symptoms of yellow vein mosaic, was characterized. HY12 was transmitted to Nicotina benthamiana by sap inoculation while the other honeysuckle isolates could not. The complete DNA-A and DNA-β like molecules of the isolate were determined and found to comprise 2763 and 1262 nucleotides (nt), respecrively, with a typical begomovirus genome organization. The DNAβ molecule had the conserved ORF (βC1) and satellite conserved regioin (SCR) typical of the Geminiviridae family containing a 5\u27-TAATATTAC-3\u27 motif in the stem loop and the adenine-(A) rich region. Additionally, its entire sequence was interrupted at intervals with sequences revealed that were not viral and whose origin was unknown. A comparison of the DNA-A sequences revealed that HY12 shared 74% with four other honeysuckle begomoviruses but 88-89% with tobacco begomoviruses found in Japan. The isolate shared up to 92% of the IR sequences with the honeysuckle begomoviruses but only 60-88% with those of tobacco. Ohylogenetic analysis of full length DNA-A component as well as its CP clusterd HY12 with the tobacco begomoviruses. HY12 is an isolate of Honeysuckle yellow vein mosaic virus