Forage Fish Populations and Growth of Muskellunge in a South Dakota Power Plant Cooling Reservoir

The standing crop, age, growth, and impingement loss of forage fishes and the growth rate and impingement loss of muskellunge (Esox masquinongy) were studied to evaluate the use of the Big Stone Power Plant cooling reservoir as an area for rearing and holding muskellunge brood stock. There were 18 species of forage fish present in the reservoir. Sampling indicated that there were 4 major forage fish species. Bluegills (Lepomis macrochirus) made up 68.3% of these species; tadpole madtoms (Noturus gyrinus), 25.8%; orangespotted sunfish (L.humilis), 3.6%; and black bullheads (Ictalurus melas), 2.3%. The estimated total standing crop of the 4 forage species was 28.1 kg/ha. The 1974 year-class was the oldest present among the forage fishes. Most forage fish populations were dominated by age-groups I and II. The growth rates of forage fishes present in the reservoir were similar to those of natural waters in the northern United States. Impingement of forage fishes was restricted predominately to young-of-the-year fishes, except during the winter months. An inverse relationship existed between impingement and monthly water temperature for adult bluegills. Higher impingement rates were noted during evening samples, but only tadpole madtoms were impinged significantly (P \u3c .05) more often at night. Muskellunge were most vulnerable to impingement during the first 2 months following their introduction. Age-group II muskellunge captured in February and March attained a mean TL of 753 mm (700 to 785 mm, range) and age-group I muskellunge captured in February and March attained a mean TL of 465 mm (386 to 575 mm:, range). The growth rate of muskellunge in the Big Stone Power Plant cooling reservoir was higher than those reported for populations throughout North America

Palmitoylation of Desmoglein 2 Is a Regulator of Assembly Dynamics and Protein Turnover.

Desmosomes are prominent adhesive junctions present between many epithelial cells as well as cardiomyocytes. The mechanisms controlling desmosome assembly and remodeling in epithelial and cardiac tissue are poorly understood. We recently identified protein palmitoylation as a mechanism regulating desmosome dynamics. In this study, we have focused on the palmitoylation of the desmosomal cadherin desmoglein-2 (Dsg2) and characterized the role that palmitoylation of Dsg2 plays in its localization and stability in cultured cells. We identified two cysteine residues in the juxtamembrane (intracellular anchor) domain of Dsg2 that, when mutated, eliminate its palmitoylation. These cysteine residues are conserved in all four desmoglein family members. Although mutant Dsg2 localizes to endogenous desmosomes, there is a significant delay in its incorporation into junctions, and the mutant is also present in a cytoplasmic pool. Triton X-100 solubility assays demonstrate that mutant Dsg2 is more soluble than wild-type protein. Interestingly, trafficking of the mutant Dsg2 to the cell surface was delayed, and a pool of the non-palmitoylated Dsg2 co-localized with lysosomal markers. Taken together, these data suggest that palmitoylation of Dsg2 regulates protein transport to the plasma membrane. Modulation of the palmitoylation status of desmosomal cadherins can affect desmosome dynamics

Fifty Years of Fisheries Management m an Obstinate Prairie Lake

Little Wall Lake, a shallow 104 ha glacial lake in Hamilton County, Iowa, has been manipulated for about 50 years by fishery managers in an attempt to provide a stable sport fishery. Managers have used dredging, water level manipulation, aquatic vegetation control, mechanical fish removal and fish eradication with toxicants, sport fish stocking, introduction of piscivorous fish, artificial habitat structures, and winter aeration. Attempts to stabilize the fishery have been unsuccessful, and because of overpopulation and stunting of panfishes, total fish eradications were conducted in 1977 and 1989. We review the history of management efforts on the lake, and suggest a series of changes in the habitat and fish community designed to achieve the goal of stabilizing the sport fishery. Proposed management actions include more extensive dredging, more intensive water level manipulations, moderate vegetation control, restrictive harvest regulations on largemouth bass (Micropterus salmoides), and aggressive use and stocking of piscivorous fish (game and non-game species) to prevent overpopulation of panfishes

Enteric Virus Survival in Package Plants and the Upgrading of the Small Treatment Plants Using Ozone

Post-chlorinated effluent collected with a portable viral concentrator from four treatment plants in Jefferson County, Kentucky, yielded infective viral particles from three plants from spring through late fall. The pH, ,chlorine, turbidity, and coliform levels of these effluents indicated that viral persistence was correlated with inefficient processing which produced effluent environments that inhibited disinfection by chlorine. The disinfection potential of ozone was tested on secondary effluent and finished water seeded with poliovirus and Esaheriahia coli. Low doses of ozone inactivated viruses and bacteria in treated water, but not in effluent. The inactivation of bacteria by ozone does not appear to be caused by cell lysis. Inability of poliovirus to form plaques correlated with inhibition of capsid penetration. Electron micrographs revealed that ozone degrades capsids. Ozonation produced low levels of COD and TOC reduction in package plant effluent. Since the reaction rates were not a simple function of COD levels and ozone dose it would be difficult to standardize dose rates. The relative inefficiency of ozone in reduction of biological and non-biological pollutants in effluents, combined with its high cost, does not favor a recommendation for ozonation as a tack-on process to upgrade these plants

Hyaluronidase Hyal1 Increases Tumor Cell Proliferation and Motility through Accelerated Vesicle Trafficking

Background: Hyal1 is a turnover enzyme for hyaluronan that accelerates metastatic cancer by increasing cell motility. Results: Hyal1-overexpressing cells have a higher rate of endocytosis that impacts cargo internalization and recycling. Conclusion: The higher rate of vesicle trafficking increases motility receptor function and nutrient uptake. Significance: This novel mechanism implicates Hyal1 trafficking in multiple signaling events during tumor progression

Designed 2D protein crystals as dynamic molecular gatekeepers for a solid-state device

The sensitivity and responsiveness of living cells to environmental changes are enabled by dynamic protein structures, inspiring efforts to construct artificial supramolecular protein assemblies. However, despite their sophisticated structures, designed protein assemblies have yet to be incorporated into macroscale devices for real-life applications. We report a 2D crystalline protein assembly of C98/E57/E66L-rhamnulose-1-phosphate aldolase (CEERhuA) that selectively blocks or passes molecular species when exposed to a chemical trigger. CEERhuA crystals are engineered via cobalt(II) coordination bonds to undergo a coherent conformational change from a closed state (pore dimensions <1 nm) to an ajar state (pore dimensions ~4 nm) when exposed to an HCN(g) trigger. When layered onto a mesoporous silicon (pSi) photonic crystal optical sensor configured to detect HCN(g), the 2D CEERhuA crystal layer effectively blocks interferents that would otherwise result in a false positive signal. The 2D CEERhuA crystal layer opens in selective response to low-ppm levels of HCN(g), allowing analyte penetration into the pSi sensor layer for detection. These findings illustrate that designed protein assemblies can function as dynamic components of solid-state devices in non-aqueous environments

Diagnosis of exercise-induced left bundle branch block at rest by scintigraphic phase analysis

Accurate diagnosis of diseases of the ventricular conducting system is essential for their appropriate therapy. some conduction abnormalities, such as exercise-induced left bundle branch block (EX-LBBB), are not apparent on resting electrocardiograms. Phase analysis of rest and exercise radionuclide ventriculograms (RVG's) was used to compare four EX-LBBB patients with six normal controls. All patients had normal resting electrocardiograms, ejection fractions, and visually normal wall motion. First harmonic phase images were generated reflecting the timing of ventricular contraction. Dynamic phase displays were reviewed and graded in a blinded fashion by three independent experienced observers. Phase angle histograms of the right and left ventricle were determined for both resting and exercise images. The mean phase angle and standard deviation were also calculated for each ventricle. Visual grading of the resting phase images failed to show a significant difference between normal patients and patients with EX-LBBB. Quantitative analysis, however, revealed a significant difference in mean phase angle differences (LV-RV) in resting studies: 0.8° (±1.9° SEM) in normals versus 9.3° (±2.3° SEM) in EX-LBBB patients ( P <0.03). Exercise accentuated the phase angle differences: 1.8° in normals vs. 31.2° in EX-LBBB patients ( P <0.001). Quantitative phase analysis of resting RVG's permits the diagnosis of cardiac conduction disease that is not apparent on the resting EKG and may result in better monitoring and treatment.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/46821/1/259_2004_Article_BF00261005.pd

embracing diversity in a strengths-based approach to promote health and equity, and avoid epistemic injustice

APP1155125. Publisher Copyright: © 2022 Author(s) (or their employer(s)).Definitions of health literacy have evolved from notions of health-related literacy to a multidimensional concept that incorporates the importance of social and cultural knowledge, practices and contexts. This evolution is evident in the development of instruments that seek to measure health literacy in different ways. Health literacy measurement is important for global health because diverse stakeholders, including the WHO, use these data to inform health practice and policy, and to understand sources of inequity. In this Practice paper, we explore the potential for negative consequences, bias and epistemic injustice to occur when health literacy instruments are used across settings without due regard for the lived experiences of people in various contexts from whom data are collected. A health literacy measurement approach that is emic-sensitive, strengths based and solution oriented is needed to minimise biased data interpretation and use and to avoid epistemic injustice.publishersversionpublishe

Estimation of TETRA radio use in the Airwave Health Monitoring Study of the British police forces.

BACKGROUND: The Airwave Health Monitoring Study aims to investigate the possible long-term health effects of Terrestrial Trunked Radio (TETRA) use among the police forces in Great Britain. Here, we investigate whether objective data from the network operator could be used to correct for misreporting in self-reported data and expand the radio usage availability in our cohort. METHODS: We estimated average monthly usage of personal radio in the 12 months prior to enrolment from a missing value imputation model and evaluated its performance against objective and self-reported data. Factors associated with TETRA radio usage variables were investigated using Chi-square tests and analysis of variance. RESULTS: The imputed data were better correlated with objective than self-reported usage (Spearman correlation coefficient = 0.72 vs. 0. 52 and kappa 0.56 [95% confidence interval 0.55, 0.56] vs. 0.46 [0.45, 0.47]), although the imputation model tended to under-estimate use for higher users. Participants with higher personal radio usage were more likely to be younger, men vs. women and officer vs. staff. The median average monthly usage level for the entire cohort was estimated to be 29.3 min (95% CI: [7.2, 66.6]). CONCLUSION: The availability of objective personal radio records for a large proportion of users allowed us to develop a robust imputation model and hence obtain personal radio usage estimates for ~50,000 participants. This substantially reduced exposure misclassification compared to using self-reported data and will allow us to carry out analyses of TETRA usage for the entire cohort in future work

Hyaluronidase Hyal1 Increases Tumor Cell Proliferation and Motility through Accelerated Vesicle Trafficking

Background: Hyal1 is a turnover enzyme for hyaluronan that accelerates metastatic cancer by increasing cell motility. Results: Hyal1-overexpressing cells have a higher rate of endocytosis that impacts cargo internalization and recycling. Conclusion: The higher rate of vesicle trafficking increases motility receptor function and nutrient uptake. Significance: This novel mechanism implicates Hyal1 trafficking in multiple signaling events during tumor progression